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Protective high-affinity antibody responses depend on competitive selection of B cells carrying somatically mutated B-cell receptors by follicular helper T (T[sub.FH]) cells in germinal centres. The rapid TB-cell interactions that occur during this process are reminiscent of neural synaptic transmission pathways. Here we show that a proportion of human T[sub.FH] cells contain dense-core granules marked by chromogranin B, which are normally found in neuronal presynaptic terminals storing catecholamines such as dopamine. T[sub.FH] cells produce high amounts of dopamine and release it upon cognate interaction with B cells. Dopamine causes rapid translocation of intracellular ICOSL (inducible T-cell co-stimulator ligand, also known as ICOSLG) to the B-cell surface, which enhances accumulation of CD40L and chromogranin B granules at the human T[sub.FH] cell synapse and increases the synapse area. Mathematical modelling suggests that faster dopamine-induced TB-cell interactions increase total germinal centre output and accelerate it by days. Delivery of neurotransmitters across the TB-cell synapse may be advantageous in the face of infection., Author(s): Ilenia Papa [1]; David Saliba [2]; Maurilio Ponzoni [3]; Sonia Bustamante [4]; Pablo F. Canete [1]; Paula Gonzalez-Figueroa [1]; Hayley A. McNamara [1]; Salvatore Valvo [2]; Michele Grimbaldeston [5, [...]

Protective high-affinity antibody responses depend on competitive selection of B cells carrying somatically mutated B-cell receptors by follicular helper T (TFH) cells in germinal centres. The rapid T-B-cell interactions that occur during this process are reminiscent of neural synaptic transmission pathways. Here we show that a proportion of human TFH cells contain dense-core granules marked by chromogranin B, which are normally found in neuronal presynaptic terminals storing catecholamines such as dopamine. TFH cells produce high amounts of dopamine and release it upon cognate interaction with B cells. Dopamine causes rapid translocation of intracellular ICOSL (inducible T-cell co-stimulator ligand, also known as ICOSLG) to the B-cell surface, which enhances accumulation of CD40L and chromogranin B granules at the human TFH cell synapse and increases the synapse area. Mathematical modelling suggests that faster dopamine-induced T-B-cell interactions increase total germinal centre output and accelerate it by days. Delivery of neurotransmitters across the T-B-cell synapse may be advantageous in the face of infection. [ABSTRACT FROM AUTHOR]