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Your search keyword '"Mulas, Ilaria"' showing total 3 results
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3 results on '"Mulas, Ilaria"'

1. Spatially resolved multiomics of human cardiac niches

Author

Kanemaru, Kazumasa, primary, Cranley, James, additional, Muraro, Daniele, additional, Miranda, Antonio M. A., additional, Ho, Siew Yen, additional, Wilbrey-Clark, Anna, additional, Patrick Pett, Jan, additional, Polanski, Krzysztof, additional, Richardson, Laura, additional, Litvinukova, Monika, additional, Kumasaka, Natsuhiko, additional, Qin, Yue, additional, Jablonska, Zuzanna, additional, Semprich, Claudia I., additional, Mach, Lukas, additional, Dabrowska, Monika, additional, Richoz, Nathan, additional, Bolt, Liam, additional, Mamanova, Lira, additional, Kapuge, Rakeshlal, additional, Barnett, Sam N., additional, Perera, Shani, additional, Talavera-López, Carlos, additional, Mulas, Ilaria, additional, Mahbubani, Krishnaa T., additional, Tuck, Liz, additional, Wang, Lu, additional, Huang, Margaret M., additional, Prete, Martin, additional, Pritchard, Sophie, additional, Dark, John, additional, Saeb-Parsy, Kourosh, additional, Patel, Minal, additional, Clatworthy, Menna R., additional, Hübner, Norbert, additional, Chowdhury, Rasheda A., additional, Noseda, Michela, additional, and Teichmann, Sarah A., additional

Published
2023
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2. A prenatal skin atlas reveals immune regulation of human skin morphogenesis

Author

Gopee, Nusayhah Hudaa, Winheim, Elena, Olabi, Bayanne, Admane, Chloe, Foster, April Rose, Huang, Ni, Botting, Rachel A., Torabi, Fereshteh, Sumanaweera, Dinithi, Le, Anh Phuong, Kim, Jin, Verger, Luca, Stephenson, Emily, Adão, Diana, Ganier, Clarisse, Gim, Kelly Y., Serdy, Sara A., Deakin, CiCi, Goh, Issac, Steele, Lloyd, Annusver, Karl, Miah, Mohi-Uddin, Tun, Win Min, Moghimi, Pejvak, Kwakwa, Kwasi Amoako, Li, Tong, Basurto Lozada, Daniela, Rumney, Ben, Tudor, Catherine L., Roberts, Kenny, Chipampe, Nana-Jane, Sidhpura, Keval, Englebert, Justin, Jardine, Laura, Reynolds, Gary, Rose, Antony, Rowe, Vicky, Pritchard, Sophie, Mulas, Ilaria, Fletcher, James, Popescu, Dorin-Mirel, Poyner, Elizabeth, Dubois, Anna, Guy, Alyson, Filby, Andrew, Lisgo, Steven, Barker, Roger A., Glass, Ian A., Park, Jong-Eun, Vento-Tormo, Roser, Nikolova, Marina Tsvetomilova, He, Peng, Lawrence, John E. G., Moore, Josh, Ballereau, Stephane, Hale, Christine B., Shanmugiah, Vijaya, Horsfall, David, Rajan, Neil, McGrath, John A., O’Toole, Edel A., Treutlein, Barbara, Bayraktar, Omer, Kasper, Maria, Progatzky, Fränze, Mazin, Pavel, Lee, Jiyoon, Gambardella, Laure, Koehler, Karl R., Teichmann, Sarah A., and Haniffa, Muzlifah

Abstract

Human prenatal skin is populated by innate immune cells, including macrophages, but whether they act solely in immunity or have additional functions in morphogenesis is unclear. Here we assembled a comprehensive multi-omics reference atlas of prenatal human skin (7–17 post-conception weeks), combining single-cell and spatial transcriptomics data, to characterize the microanatomical tissue niches of the skin. This atlas revealed that crosstalk between non-immune and immune cells underpins the formation of hair follicles, is implicated in scarless wound healing and is crucial for skin angiogenesis. We systematically compared a hair-bearing skin organoid (SkO) model derived from human embryonic stem cells and induced pluripotent stem cells to prenatal and adult skin1. The SkO model closely recapitulated in vivo skin epidermal and dermal cell types during hair follicle development and expression of genes implicated in the pathogenesis of genetic hair and skin disorders. However, the SkO model lacked immune cells and had markedly reduced endothelial cell heterogeneity and quantity. Our in vivo prenatal skin cell atlas indicated that macrophages and macrophage-derived growth factors have a role in driving endothelial development. Indeed, vascular network remodelling was enhanced following transfer of autologous macrophages derived from induced pluripotent stem cells into SkO cultures. Innate immune cells are therefore key players in skin morphogenesis beyond their conventional role in immunity, a function they achieve through crosstalk with non-immune cells.

Published
2024
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3. A multi-omic atlas of human embryonic skeletal development.

Author

To K, Fei L, Pett JP, Roberts K, Blain R, Polański K, Li T, Yayon N, He P, Xu C, Cranley J, Moy M, Li R, Kanemaru K, Huang N, Megas S, Richardson L, Kapuge R, Perera S, Tuck E, Wilbrey-Clark A, Mulas I, Memi F, Cakir B, Predeus AV, Horsfall D, Murray S, Prete M, Mazin P, He X, Meyer KB, Haniffa M, Barker RA, Bayraktar O, Chédotal A, Buckley CD, and Teichmann SA

Subjects
Humans, Atlases as Topic, Cell Lineage genetics, Chondrocytes cytology, Chondrocytes metabolism, Epigenesis, Genetic, Gene Expression Regulation, Developmental, Gene Regulatory Networks, Joints cytology, Joints embryology, Joints metabolism, Osteoarthritis genetics, Osteoarthritis metabolism, Osteoarthritis pathology, Schwann Cells cytology, Schwann Cells metabolism, Skull embryology, Skull cytology, Transcriptome, Embryonic Development genetics, Multiomics, Osteogenesis genetics
Abstract

Human embryonic bone and joint formation is determined by coordinated differentiation of progenitors in the nascent skeleton. The cell states, epigenetic processes and key regulatory factors that underlie lineage commitment of these cells remain elusive. Here we applied paired transcriptional and epigenetic profiling of approximately 336,000 nucleus droplets and spatial transcriptomics to establish a multi-omic atlas of human embryonic joint and cranium development between 5 and 11 weeks after conception. Using combined modelling of transcriptional and epigenetic data, we characterized regionally distinct limb and cranial osteoprogenitor trajectories across the embryonic skeleton and further described regulatory networks that govern intramembranous and endochondral ossification. Spatial localization of cell clusters in our in situ sequencing data using a new tool, ISS-Patcher, revealed mechanisms of progenitor zonation during bone and joint formation. Through trajectory analysis, we predicted potential non-canonical cellular origins for human chondrocytes from Schwann cells. We also introduce SNP2Cell, a tool to link cell-type-specific regulatory networks to polygenic traits such as osteoarthritis. Using osteolineage trajectories characterized here, we simulated in silico perturbations of genes that cause monogenic craniosynostosis and implicate potential cell states and disease mechanisms. This work forms a detailed and dynamic regulatory atlas of bone and cartilage maturation and advances our fundamental understanding of cell-fate determination in human skeletal development., Competing Interests: Competing interests: C.D.B. is a founder of Mestag Therapeutics. S.A.T. is a scientific advisory board member of ForeSite Labs, OMass Therapeutics, Qiagen, a co-founder and equity holder of TransitionBio and EnsoCell Therapeutics, a non-executive director of 10x Genomics and a part-time employee of GlaxoSmithKline. Sarah also holds equity in TransitionBio and options for equity in Element Biosciences, where she is a former SAB member.  All other authors declare no competing interests., (© 2024. The Author(s).)

Published
2024
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