Search

In a multicellular organism, normal growth requires control of cell division to generate cells that are similar to or different from their parents. Analysis of this process in plant roots reveals how this mechanism is regulated.What governs which of two types of cell division occurs in plants. [ABSTRACT FROM AUTHOR]

Dynamically polarized membrane proteins define different cell boundaries and have an important role in intercellular communication--a vital feature of multicellular development. Efflux carriers for the signalling molecule auxin from the [...]

Author(s): Yuchen Long [1, 2]; Yvonne Stahl [3]; Stefanie Weidtkamp-Peters [4]; Marten Postma [5]; Wenkun Zhou [1]; Joachim Goedhart [5]; Mara-Isabel Snchez-Prez [6]; Theodorus W. J. Gadella [5]; Rdiger Simon [...]

Factors with a graded distribution can program fields of cells in a dose-dependent manner (1,2), but no evidence has hitherto surfaced for such mechanisms in plants. In the Arabidopsis thaliana [...]

Author(s): Ikram Blilou [1]; Jian Xu [1, 4]; Marjolein Wildwater [1, 4]; Viola Willemsen [1, 4]; Ivan Paponov [2]; Jiří Friml [3]; Renze Heidstra [1]; Mitsuhiro Aida [1]; Klaus Palme [...]

During multicellular development, specification of distinct cell fates is often regulated by the same transcription factors operating differently in distinct cis-regulatory modules, either through different protein complexes, conformational modification of protein complexes, or combinations of both. Direct visualization of different transcription factor complex states guiding specific gene expression programs has been challenging. Here we use in vivo FRET-FLIM (Förster resonance energy transfer measured by fluorescence lifetime microscopy) to reveal spatial partitioning of protein interactions in relation to specification of cell fate. We show that, in Arabidopsis roots, three fully functional fluorescently tagged cell fate regulators establish cell-type-specific interactions at endogenous expression levels and can form higher order complexes. We reveal that cell-type-specific in vivo FRET-FLIM distributions reflect conformational changes of these complexes to differentially regulate target genes and specify distinct cell fates. [ABSTRACT FROM AUTHOR]