Search

Your search keyword '"Romeo LR"' showing total 1 results
Start Over Author "Romeo LR" Journal nanotoxicology

Refine your results

1 results on '"Romeo LR"'

1. Mechanisms of toxicity induced by SiO2 nanoparticles of in vitro human alveolar barrier: effects on cytokine production, oxidative stress induction, surfactant proteins A mRNA expression and nanoparticles uptake.

Author

Farcal LR, Uboldi C, Mehn D, Giudetti G, Nativo P, Ponti J, Gilliland D, Rossi F, and Bal-Price A

Subjects
Cell Line, Cell Survival, Electric Impedance, Endothelial Cells drug effects, Endothelial Cells metabolism, Epithelial Cells drug effects, Epithelial Cells metabolism, Humans, Lipopolysaccharides, Macrophages physiology, Monocytes physiology, Nanoparticles chemistry, Pulmonary Surfactant-Associated Protein A genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Reactive Oxygen Species, Silicon Dioxide chemistry, Cytokines metabolism, Nanoparticles toxicity, Oxidative Stress drug effects, Pulmonary Alveoli drug effects, Pulmonary Surfactant-Associated Protein A metabolism, Silicon Dioxide toxicity
Abstract

An in vitro human alveolar barrier established by the coculture of epithelial human cell line NCI-H441 with endothelial human cell line ISO-HAS1 was used to evaluate the effects of amorphous silicon dioxide nanoparticles (SiNPs), in the presence or absence of THP-1 cells (monocytes). SiNPs exposure induced production of proinflammatory cytokine and oxidative stress. A high release of TNF-α and IL-8 by epithelial/endothelial cells, potentiated in the presence of THP-1 cells could contribute to the observed downregulation of surfactant proteins A mRNA expression resulting in the damage of the alveolar barrier. The obtained results suggested that in vitro approach can be used to study pulmonary toxicity as long as the applied in vitro model mimics closely the complexity of in vivo situation.

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results