1. Nanoparticles based on disulfide-containing poly(β-amino ester) and zwitterionic fluorocarbon surfactant as a redox-responsive drug carrier for brain tumor treatment
- Author
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Ping Zhou, Yinsong Wang, Hua Yan, Qian Chen, and Ruoyang Feng
- Subjects
0301 basic medicine ,Materials science ,Cell Survival ,Polymers ,Nanoparticle ,Apoptosis ,Bioengineering ,02 engineering and technology ,Cell Line ,Surface-Active Agents ,03 medical and health sciences ,Pulmonary surfactant ,Cell Line, Tumor ,medicine ,Humans ,General Materials Science ,Doxorubicin ,Electrical and Electronic Engineering ,Fluorocarbons ,Antibiotics, Antineoplastic ,Brain Neoplasms ,Cell growth ,Mechanical Engineering ,General Chemistry ,021001 nanoscience & nanotechnology ,In vitro ,030104 developmental biology ,Blood-Brain Barrier ,Mechanics of Materials ,Cell culture ,Delayed-Action Preparations ,Biophysics ,Nanoparticles ,0210 nano-technology ,Drug carrier ,Oxidation-Reduction ,medicine.drug - Abstract
Malignant brain tumors are often characterized by rapid growth, high invasiveness and poor prognosis. Current methods for brain tumor treatment are dramatically limited because of their inability to cross the blood-brain barrier (BBB) and enter the tumor cells. In this study, we prepared redox-responsive nanoparticles based on disulfide-containing poly(β-amino ester) (ssPBAE) and a zwitterionic fluorocarbon surfactant (Intechem-02) that has a carboxybetaine moiety in molecular structure, and preliminarily evaluated their potential as a drug carrier for brain tumor treatment. These nanoparticles, named as ssPBAEI, had a regular spherical shape and a small size below 50 nm with a relative narrow distribution. Doxorubicin (DOX), as a model chemotherapeutic drug, was efficiently encapsulated into ssPBAEI nanoparticles with a loading content of 25.4%. DOX-loaded ssPBAEI nanoparticles (ssPBAEI/DOX) showed significant redox-responsive in vitro release property and successfully carried DOX across a BBB model, monolayer of human brain capillary endothelial hCMEC/D3 cells. In human glioma LN229 cells, ssPBAEI/DOX nanoparticles were efficiently internalized and DOX was successfully released afterwards, thus significantly inhibited cell growth and induced cell apoptosis. In summary, this nanoparticle system based on ssPBAE and Intechem-02 showed a great potential as a drug carrier for brain tumor treatment.
- Published
- 2018
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