1. Zwitterionic surface coating of quantum dots reduces protein adsorption and cellular uptake
- Author
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Neus Feliu, Joonhyuck Park, Dakyeon Lee, Sungjee Kim, Junhwa Lee, Alaa Hassan Said, Sumaira Ashraf, Karsten Kantner, Wolfgang J. Parak, Gerd Ulrich Nienhaus, Raimo Hartmann, Pauline Maffre, and Mathilde A. Bichelberger
- Subjects
Materials science ,media_common.quotation_subject ,Analytical chemistry ,Nanoparticle ,Serum Albumin, Human ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,HeLa ,Adsorption ,Quantum Dots ,medicine ,Humans ,General Materials Science ,Internalization ,media_common ,biology ,technology, industry, and agriculture ,Biological Transport ,equipment and supplies ,021001 nanoscience & nanotechnology ,Human serum albumin ,biology.organism_classification ,0104 chemical sciences ,Surface coating ,Quantum dot ,Biophysics ,0210 nano-technology ,HeLa Cells ,Protein adsorption ,medicine.drug - Abstract
We have studied the effect of the zwitterionic surface coating of quantum dots (QDs) on their interaction with a serum supplemented cell medium and their internalization by human cervical carcinoma (HeLa) cells. Zwitterionic QDs showed negligible adsorption of human serum albumin (HSA) selected as a model serum protein, in contrast to similar but negatively charged QDs. The incorporation of zwitterionic QDs by HeLa cells was found to be lower than for negatively charged QDs and for positively charged QDs, for which the uptake yield was largest. Our results suggest that the suppression of protein adsorption, here accomplished by zwitterionic QD surfaces, offers a strategy that allows for reducing the cellular uptake of nanoparticles.
- Published
- 2016
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