1. Pharmacological Dose-Effect Profiles of Various Concentrations of Humanised Primary Bile Acid in Encapsulated Cells.
- Author
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Mooranian, Armin, Jones, Melissa, Walker, Daniel, Ionescu, Corina Mihaela, Wagle, Susbin Raj, Kovacevic, Bozica, Chester, Jacqueline, Foster, Thomas, Johnston, Edan, Kuthubutheen, Jafri, Brown, Daniel, Atlas, Marcus D., Mikov, Momir, and Al-Salami, Hani
- Subjects
BILE acids ,CHENODEOXYCHOLIC acid ,CELL survival ,CELL physiology ,CELL analysis - Abstract
Bile acids (BA)s are known surfactants and well-documented to play a major role in food digestion and absorption. Recently, potential endocrinological and formulation-stabilisation effects of BAs have been explored and their pharmacological effects on supporting cell survival and functions have gained wide interest. Hence, this study aimed to explore the hyper-glycaemic dependent dose-effect of the BA chenodeoxycholic acid (CDCA) when encapsulated with pancreatic β-cells, allowing assessment of CDCA's impacts when encapsulated. Four different concentrations of the BA were prepared, and viable cells were encapsulated and incubated for 2 days. Multiple analyses were carried out including confocal imaging, glucose-induced cellular mitochondrial viability indices, insulin production, inflammatory biomarker analyses and cellular bioenergetics measurements. There was a significant dose-effect with different concentrations of the BA, affecting cellular viability and antioxidant activities, cell functions and insulin release, inflammatory biomarkers, and cellular-bioenergetics at different oxidative stress levels. The results demonstrate that, when encapsulated, the BA CDCA exerts positive pharmacological effects at the cellular level, and such effects are concentration dependent. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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