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We generated, on titanium surfaces, self-assembled layers of vertically oriented TiO2 nanotubes with defined diameters between 15 and 100 nm and show that adhesion, spreading, growth, and differentiation of mesenchymal stem cells are critically dependent on the tube diameter. A spacing less than 30 nm with a maximum at 15 nm provided an effective length scale for accelerated integrin clustering/focal contact formation and strongly enhances cellular activities compared to smooth TiO2 surfaces. Cell adhesion and spreading were severely impaired on nanotube layers with a tube diameter larger than 50 nm, resulting in dramatically reduced cellular activity and a high extent of programmed cell death. Thus, on a TiO2 nanotube surface, a lateral spacing geometry with openings of 30-50 nm represents a critical borderline for cell fate.

In the present work we show a simple and robust fabrication process of a dense and free-standing membrane consisting of vertically oriented, both-side-open TiO2 nanotubes. This membrane structure allows direct, size-selective, flow-through photocatalytic reactions with a very high efficiency.

We present kinetics data of O2, n/iso-butane, CO2, and CO adsorbed at ultrahigh vacuum conditions on TiO2 nanotube (TiNTs) arrays produced by electrochemical anodization; amorphous and polycrystalline (anatase and mixed anatase/rutile) TiNTs have been studied addressing structure-activity relationships. Oxygen distinctly interacts with the TiNTs, whereas this process is not observed on fully oxidized TiO2 single crystals. Both molecularly and atomically bonded oxygen have been observed. Variations in the binding energies of alkanes were also detected.

We apply high-energy proton ion-implantation to modify TiO2 nanotubes selectively at their tops. In the proton-implanted region, we observe the creation of intrinsic cocatalytic centers for photocatalytic H2-evolution. We find proton implantation to induce specific defects and a characteristic modification of the electronic properties not only in nanotubes but also on anatase single crystal (001) surfaces. Nevertheless, for TiO2 nanotubes a strong synergetic effect between implanted region (catalyst) and implant-free tube segment (absorber) can be obtained.

Here we report that TiO2 nanotube (NT) arrays, converted by a high pressure H2 treatment to anatase-like "black titania", show a high open-circuit photocatalytic hydrogen production rate without the presence of a cocatalyst. Tubes converted to black titania using classic reduction treatments (e.g., atmospheric pressure H2/Ar annealing) do not show this effect. The main difference caused by the high H2 pressure annealing is the resulting room-temperature stable, isolated Ti(3+) defect-structure created in the anatase nanotubes, as evident from electron spin resonance (ESR) investigations. This feature, absent for conventional reduction, seems thus to be responsible for activating intrinsic, cocatalytic centers that enable the observed high open-circuit hydrogen generation.

Self-organized anodic titania nanotube layers were doped with nitrogen successfully using ion implantation. Photoelectrochemical measurements combined with XRD measurements show that the damage created by ion bombardment (that leads to a drastic decrease of the photoconversion efficiency) can be “annealed out” by an adequate heat treatment. This results in a N-doped crystalline anatase nanotube structure with strongly enhanced photocurrent response in both the UV and the visible range.

Critical features of biomimetic materials used for vascular grafts and stents are surface structure and chemical features of the implant material supporting adhesion, proliferation, and differentiation of endothelial cells and smooth muscle cells, the major cell types of blood vessels. Recently, experimental evidence from several laboratories have indicated a strong stimulation of cellular activities on vertically aligned TiO(2) nanotube surfaces in comparison to amorphous TiO(2) surfaces. Conflicting reports exist, however, concerning the nanoscale dimension, and the role of the chemistry and crystallinity of the nanotubes in eliciting cell responses. Here we demonstrate that 15 nm nanotubes provide a substantially stronger stimulation of differentiation of mesenchymal cells to endothelial cells and smooth muscle cells than 70-100 nm nanotubes, while high rates of apoptosis were seen on 100 nm nanotubes. Also endothelial cell adhesion, proliferation, and motility were several-fold higher on 15 nm than on 100 nm nanotubes. Furthermore, our data indicate a clear dominance of the nanoscale geometry on endothelial cell behavior over surface chemistry and crystallinity of the TiO(2) nanotube surface. These findings indicate that fine-tuning of TiO(2) surfaces at nanoscale will be an essential parameter in optimizing endothelial cell and smooth muscle cell responses to vascular implants.