1. Axon-First Neuritogenesis on Vertical Nanowires
- Author
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Kyungtae Kang, Myung-Han Yoon, Yoonkey Nam, Juno Lee, Min Jee Jang, Insung S. Choi, Da Jee Jung, Seong-Min Kim, Young-Tae Chang, Yi Seul Park, Matthew Park, Jin Seok Lee, and Ji Yu Choi
- Subjects
Silicon ,Neurite ,Neurogenesis ,Nanowire ,Cell Culture Techniques ,Bioengineering ,Nanotechnology ,02 engineering and technology ,Hippocampal formation ,010402 general chemistry ,01 natural sciences ,Hippocampus ,In vivo ,Live cell imaging ,medicine ,Neurites ,Animals ,General Materials Science ,Axon ,Cytoskeleton ,Chemistry ,Nanowires ,Mechanical Engineering ,General Chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Actins ,Axons ,0104 chemical sciences ,Rats ,medicine.anatomical_structure ,Cell Tracking ,Biophysics ,Lamellipodium ,0210 nano-technology - Abstract
In this work, we report that high-density, vertically grown silicon nanowires (vg-SiNWs) direct a new in vitro developmental pathway of primary hippocampal neurons. Neurons on vg-SiNWs formed a single, extremely elongated major neurite earlier than minor neurites, which led to accelerated polarization. Additionally, the development of lamellipodia, which generally occurs on 2D culture coverslips, was absent on vg-SiNWs. The results indicate that surface topography is an important factor that influences neuronal development and also provide implications for the role of topography in neuronal development in vivo.
- Published
- 2015