1. CD44 Antibody-Conjugated Gold Nanostars as SERS Probes for Distinguishing Cancer Cells (A549 Cells, H1229 Cells) from Normal Cells (ATII Cells).
- Author
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Zhao, Hang, Cao, Xiaowei, Wang, Man, Tao, Lin, Pan, Xiaoyu, Yuan, Chunwei, and Qian, Weiping
- Subjects
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CD44 antigen , *GOLD nanoparticles , *IMMUNOGLOBULINS , *MOLECULAR probes , *CANCER cells , *CELLULAR signal transduction - Abstract
In this paper, we report a novel antibody conjugated gold nanostars () as surface-enhanced Raman spectroscopy (SERS) probes for distinguishing cancer cells (A549 cells, H1229 cells) from normal cells (ATII cells). In such a probe, bovine serum albumin (BSA) was served as the protective agent and stabilizing agent, 4-mercaptobenzoic acid (4-MBA) was used as the Raman reporter to generate SERS signals as well as the conjugation agent for attaching CD44 antibody (anti-CD44) molecules to , where anti-CD44 could effectively target to CD44 protein over-expressed cells. All the results of the experiments confirmed that more SERS probes have been targeted to cancer cells (A549 cells and H1229 cells) than that of normal cells (ATII cells) under the same condition. The anti-CD44 SERS probes hold a potential application in distinguishing cancer cells from normal cells with high sensitivity and good biocompatibility. BSA-stabilized Au nanostars were synthesized with a seed-mediated growth approach in two steps, in which trisodium citrate dehydrate was used as the reducing agent. In SERS probes, 4-MBA was absorbed on the surface of Au nanostars as both the Raman reporter molecule and the conjugation agent for antibody, which could target to different cells through the combination of featured proteins on the cell membrane. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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