Search

Your search keyword '"J. Hitchcock"' showing total 4 results
Start Over Author "J. Hitchcock" Journal mycological research
4 results on '"J. Hitchcock"'

1. Ectomycorrhizal mycobionts of Pisonia grandis on coral cays in the Capricorn-Bunker group, Great Barrier Reef, Australia

Author

John W.G. Cairney, Catherine J. Hitchcock, and Susan M. Chambers

Subjects
Thelephoraceae, Coral, Plant Science, Biology, Plant Roots, Polymerase Chain Reaction, Genus, Mycorrhizae, DNA, Ribosomal Spacer, Botany, Genetics, Animals, DNA, Fungal, Phylogeny, Soil Microbiology, Ecology, Evolution, Behavior and Systematics, Ecology, Basidiomycota, Fungal genetics, Nyctaginaceae, Anthozoa, biology.organism_classification, Ectomycorrhiza, Queensland, Tomentella, Pisonia grandis, Polymorphism, Restriction Fragment Length, Biotechnology
Abstract

The diversity of ectomycorrhizal mycobionts of Pisonia grandis (Nyctaginaceae) from coral cays in the Capricorn-Bunker group, Great Barrier Reef, Australia, was examined. Only two ectomycorrhiza morphotypes (brown and black) were identified in soil from seven cays and DNA from both morphotypes was subjected to ITS-RFLP and sequence analysis. The brown morphotype was present in soil from all cays but the black morphotype was only observed in soil from three cays. ITS-RFLP analysis showed that the brown and black morphotypes were formed by different fungal taxa, with the RFLP pattern for the black morphotype being consistent with that of the culture previously obtained from black ectomycorrhizal roots on Heron Island. Comparison with the GenBank database revealed that closest matches to both morphotypes were sequences for various Thelephoraceae (Basidiomycota), but the brown and black morphotypes had only 80% sequence similarity to each other. Neighbour-joining analysis of these sequences with sequences for other Thelephoraceae grouped the brown and black morphotypes in a well-supported clade with several Tomentella species, suggesting that both belong to this genus. The data are discussed in relation to ectomycorrhizal fungal diversity and the coral cay habitat.

Published
2005
Full Text
View/download PDF

2. Development of markers for simple sequence repeat-rich regions that discriminate between Pisolithus albus and P. microcarpus

Author

Ian C. Anderson, John W.G. Cairney, Catherine J. Hitchcock, and Susan M. Chambers

Subjects
Genetic Markers, Minisatellite Repeat, Locus (genetics), Plant Science, Minisatellite Repeats, Pisolithus, Polymerase Chain Reaction, law.invention, chemistry.chemical_compound, law, Molecular marker, Botany, Genetics, Allele, DNA, Fungal, Mycological Typing Techniques, Ecology, Evolution, Behavior and Systematics, Polymerase chain reaction, Polymorphism, Genetic, biology, Basidiomycota, UPGMA, Sequence Analysis, DNA, biology.organism_classification, chemistry, Genetic marker, Polymorphism, Restriction Fragment Length, Biotechnology
Abstract

Inter-simple sequence repeat PCR (ISSR-PCR) was used to develop markers for simple sequence repeat-rich (SSR) regions for investigation of genetic relatedness of Pisolithus isolates collected from eastern mainland Australia. Primers were designed to amplify ten SSR-rich regions and these were used to screen 14 Pisolithus isolates. Two amplified loci showed size polymorphisms among the isolates (regarded as polymorphic), two were monomorphic for all isolates, while the remainder amplified alleles for only some isolates. UPGMA analysis of the alleles for each isolate at each locus together with ITS-RFLP analysis, separated the isolates into groups. These two groups appear to correspond to isolates that ITS sequence data have previously separated as P. albus and P. microcarpus.

Published
2003

3. Ectomycorrhizal mycobionts of Pisonia grandis on coral cays in the Capricorn-Bunker group, Great Barrier Reef, Australia.

Author

Susan M. CHAMBERS, Catherine J. HITCHCOCK, and John W. G. CAIRNEY

Subjects
*ECTOMYCORRHIZAS, *NYCTAGINACEAE, *PISONIA, *CORALS, *BASIDIOMYCOTA
Abstract

The diversity of ectomycorrhizal mycobionts of Pisonia grandis (Nyctaginaceae) from coral cays in the Capricorn-Bunker group, Great Barrier Reef, Australia, was examined. Only two ectomycorrhiza morphotypes (brown and black) were identified in soil from seven cays and DNA from both morphotypes was subjected to ITS-RFLP and sequence analysis. The brown morphotype was present in soil from all cays but the black morphotype was only observed in soil from three cays. ITS-RFLP analysis showed that the brown and black morphotypes were formed by different fungal taxa, with the RFLP pattern for the black morphotype being consistent with that of the culture previously obtained from black ectomycorrhizal roots on Heron Island. Comparison with the GenBank database revealed that closest matches to both morphotypes were sequences for various Thelephoraceae (Basidiomycota), but the brown and black morphotypes had only 80% sequence similarity to each other. Neighbour-joining analysis of these sequences with sequences for other Thelephoraceae grouped the brown and black morphotypes in a well-supported clade with several Tomentella species, suggesting that both belong to this genus. The data are discussed in relation to ectomycorrhizal fungal diversity and the coral cay habitat. [ABSTRACT FROM AUTHOR]

Published
2005
Full Text
View/download PDF

4. Development of markers for simple sequence repeat-rich regions that discriminate between Pisolithus albus and P. microcarpus.

Author

Catherine J. HITCHCOCK and Susan M. CHAMBERS

Subjects
*GENETICS, *GENETIC polymorphisms, *ALLELES
Abstract

Inter-simple sequence repeat PCR (ISSR-PCR) was used to develop markers for simple sequence repeat-rich (SSR) regions for investigation of genetic relatedness of Pisolithus isolates collected from eastern mainland Australia. Primers were designed to amplify ten SSR-rich regions and these were used to screen 14 Pisolithus isolates. Two amplified loci showed size polymorphisms among the isolates (regarded as polymorphic), two were monomorphic for all isolates, while the remainder amplified alleles for only some isolates. UPGMA analysis of the alleles for each isolate at each locus together with ITS-RFLP analysis, separated the isolates into two groups. These two groups appear to correspond to isolates that ITS sequence data have previously separated as P. albus and P. microcarpus. [ABSTRACT FROM AUTHOR]

Published
2003
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results