1. Effect of vitamin levels on biomarkers of exposure and oxidative damage-the EXPAH study
- Author
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Ivan Kalina, Rajinder Singh, Emanuela Taioli, Radim J. Sram, Peter B. Farmer, Seymour Garte, Blanka Binkova, Todor A. Popov, and Camille Ragin
- Subjects
Vitamin ,Adult ,Male ,medicine.medical_specialty ,DNA damage ,Health, Toxicology and Mutagenesis ,medicine.medical_treatment ,medicine.disease_cause ,03 medical and health sciences ,chemistry.chemical_compound ,DNA Adducts ,0302 clinical medicine ,Internal medicine ,Surveys and Questionnaires ,DNA adduct ,Genetics ,medicine ,Deoxyguanosine ,Humans ,Carcinogen ,030304 developmental biology ,Aged ,0303 health sciences ,Chemistry ,Vitamin E ,Vitamins ,Middle Aged ,3. Good health ,Oxidative Stress ,Endocrinology ,Cross-Sectional Studies ,Biochemistry ,030220 oncology & carcinogenesis ,Benzopyrene ,Female ,Oxidative stress ,Biomarkers - Abstract
DNA adducts are markers of carcinogen exposure and of their biological effect; they have been shown to be related to mutagenesis, and therefore they could be a predictive biomarker of human cancer. The objective of this study was to assess if there is a relationship between vitamins A, C, and E, which are known to play a significant role as free radical scavengers and antioxidant agents, and biomarkers of genotoxicity and oxidative stress. Three hundred and fifty-six subjects from Czech Republic, Slovak Republic and Bulgaria, who completed a questionnaire on dietary information and had a measurement of plasma A, C, E vitamins, DNA adduct levels (benzo[a]pyrene (B[a]P) and bulky (DNA-Tot) DNA adducts) and oxidative damage (cyclic pyrimidopurinone N-1,N2 malondialdehyde-2 deoxyguanosine (M1dG) and 8-oxo-7,8-dihydro-2_deoxyguanosine (8-oxodG)) were analyzed. A significant inverse correlation was observed between plasma vitamin levels and both benzo[a]pyrene (B[a]P) and bulky DNA adducts. Vitamin A was also significantly inversely correlated with M1dG, a marker of oxidative damage. The associations were stronger in non-smokers than in smokers. Dietary intake of certain antioxidants such as vitamins is associated with reduced levels of markers of DNA damage (B[a]P and DNA-Tot) and oxidation (M1dG and 8-oxodG) measured in peripheral white blood cells. This could contribute to the protective role of such a dietary pattern on cancer risk. The protective effect of dietary vitamins is less evident in smokers.
- Published
- 2008