1. Evidence for a parent-of-origin effect on sperm aneuploidy in mice carrying Robertsonian translocations as analyzed by fluorescence in situ hybridization.
- Author
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Baulch JE, Lowe XR, Bishop JB, and Wyrobek AJ
- Subjects
- Animals, Chimera, Female, Heterozygote, Male, Mice, X Chromosome genetics, Aneuploidy, In Situ Hybridization, Fluorescence methods, Spermatids physiology, Translocation, Genetic genetics
- Abstract
Multi-color fluorescence in situ hybridization (FISH) was employed to investigate variations in the frequency of aneuploid spermatids produced by males derived from three separate lines of Robertsonian translocations in mice: Rb(2.8)2Lub, Rb(8.12)22Lub, and Rb(8.14)16Rma, each with one arm involving chromosome 8. The DNA probes used were specific for repetitive sequences on chromosomes 8 and X. Heterozygous males for these Robertsonian translocations produced approximately 1% of spermatids with hyperhaploid for chromosome 8. which was > 80 times higher than the frequency of sperm hyperhaploid for chromosome X within the same animals; consistent elevations in chromosome-8 sperm disomy were observed among lines. In addition, approximately 25% higher fractions of sperm aneuploidy were observed when the Robertsonian translocation was inherited from the father rather than from the mother (p = 0.009). These findings illustrate the sensitivity of the FISH procedure for detecting small differences in the hyperhaploidy in male germ cells and suggest that imprinted factors may influence sperm aneuploidy.
- Published
- 1996
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