1. Pyrogenic synthetic amorphous silica (NM-203): Genotoxicity in rats following sub-chronic oral exposure
- Author
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Paola Villani, Patrizia Eleuteri, Francesca Pacchierotti, Francesca Maranghi, Roberta Tassinari, Laura Narciso, Sabrina Tait, Gabriele Lori, Cristina Andreoli, Sylvie Huet, Gérard Jarry, Valérie Fessard, Eugenia Cordelli, Agenzia Nazionale per le nuove Tecnologie, l’energia e lo sviluppo economico sostenibile = Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Istituto Superiore di Sanità (ISS), Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and European Project: 310584,EC:FP7:NMP,FP7-NMP-2012-LARGE-6,NANOREG(2013)
- Subjects
Male ,Health, Toxicology and Mutagenesis ,MESH: Mutagenicity Tests ,nanoparticule ,Rats, Sprague-Dawley ,comet assay ,Genetics ,Animals ,toxicité ,rat ,Synthetic amorphous silica ,silice synthétique amorphe ,food additive ,MESH: DNA Damage ,silicon dioxide ,Micronucleus Tests ,nanoparticle ,additif ,genotoxicity ,toxicity ,toxicologie ,test des comètes ,Rats ,dioxine de silicone ,micronucleus test ,sécruité des aliments ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,génotoxicité ,DNA damage ,Female ,test du micronucleus ,MESH: Nanoparticles ,toxicology - Abstract
International audience; The genotoxicity of nano-structured synthetic amorphous silica (SAS), a common food additive, was investigated in vivo in rats. A 90-day oral toxicity study was performed according to OECD test guideline 408 and the genotoxicity of pyrogenic SAS nanomaterial NM-203 was assessed in several organs, using complementary tests. Adult Sprague-Dawley rats of both sexes were treated orally for 90 days with 0, 2, 5, 10, 20, or 50 mg SAS/kg bw per day. Dose levels were selected to approximate expected human dietary exposures to SAS. DNA strand breaks were evaluated by the comet assay in blood, bone marrow, liver, and spleen according to OECD test guideline 489; mutations induced in bone marrow precursors of erythrocytes were assessed by the Pig-a assay and chromosome/ genome damage by the micronucleus assay in blood (OECD test guideline 474) and colon. No treatment-related increases of gene (Pig-a) or chromosome/genome (micronucleus) mutations were detected in the blood. The percentage of micronucleated cells was not increased in the colon of treated rats. Among the organs analyzed by the comet assay, the spleen was the only target showing a weak but biologically relevant genotoxic effect.
- Published
- 2022