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Start Over Author "Mevkh AT" Journal molekuliarnaia biologiia
6 results on '"Mevkh AT"'

1. [Kinetic model of a multienzyme system of blood prostanoid synthesis. I. Mechanism of stabilization of thromboxane and prostacyclin levels]

Author

S D, Varfolomeev, A T, Mevkh, and V P, Gachok

Subjects
Blood Platelets, Kinetics, Thromboxane A2, Arachidonic Acid, Multienzyme Complexes, Phospholipases, Prostaglandin-Endoperoxide Synthases, Humans, Arachidonic Acids, Epoprostenol, Models, Biological, Substrate Specificity
Abstract

A kinetic scheme of the prostacyclin-thromboxane system has been evolved on the basis of the authors experimental data and the results described elsewhere. The kinetic behavior of the model has been analysed with the aid of computer technology by varying the following parameters: phospholipase activities, free arachidonic acid exchange rates between platelets and endothelium, PGH-synthetase biosynthesis rates, velocities of arachidonic acid pathways other than the cyclooxygenase ones. It has been demonstrated that the biological system is capable of sustaining prostacyclin and thromboxane concentrations at steady fixed levels within a wide range of kinetic parameters.

Published
1986

2. [Kinetic model of a multienzyme system of blood prostanoid synthesis. II. Dynamic responses to pharmacological agents--inhibitors of prostaglandin H synthetases]

Author

S D, Varfolomeev, A T, Mevkh, and V P, Gachok

Subjects
Blood Platelets, Kinetics, Thromboxane A2, Arachidonic Acid, Multienzyme Complexes, Anti-Inflammatory Agents, Non-Steroidal, Humans, Cyclooxygenase Inhibitors, Arachidonic Acids, Endothelium, Epoprostenol, Models, Biological
Abstract

The dynamic replies of the multienzyme system of blood prostanoid synthesis to the introduction of an irreversible inhibitor of prostaglandin H synthetase (PGH synthetase) have been analysed by using kinetic modelling. The alterations of arachidonic acid and PGH synthetase concentrations in platelets and endothelium and the concentrations of thromboxane and prostacyclin have been demonstrated. Particularities of kinetic behaviour of the system probably providing the therapeutic effect of non-steroidal anti-inflammatory drugs have been shown. Namely, the kinetic wave of free arachidonic acid and prostacyclin concentration with respect to thromboxane concentration appears after introduction of the drugs.

Published
1986

3. [Kinetic model and mechanism of regulation of a multienzyme system of thromboxane synthesis]

Author

S D, Varfolomeev, V P, Gachok, and A T, Mevkh

Subjects
Kinetics, Arachidonic Acid, Multienzyme Complexes, Phospholipases, Thromboxanes, Cyclooxygenase Inhibitors, Arachidonic Acids, In Vitro Techniques, Models, Biological
Abstract

A kinetic model has been suggested for the thromboxane synthesis polyenzyme system, taking into account the inactivation of a limiting enzyme, prostaglandin H-synthetase, in the course of the reaction. The model also includes the effect of phospholipase, adenylate cyclase, as well as the "outflows" of the components from the system, and basic regulatory effects in the system. A mathematical description of the model is given, and numerical solutions of the system of equations obtained for a wide variety of parameters. An analysis of the kinetic responses of the polyenzyme system shows that: 1) the system is highly conserved with respect to thromboxane concentration changes; 2) two cardinally different modes of the system are registered; they correspond to two insignificantly differing thromboxane steady-state levels; 3) transition of the system from one mode to the other is controlled by the phospholipase activity level which is sensitive to a change in the concentration of the regulator (cyclo-AMP or Ca ions) in the system. It is presumed that the two thromboxane steady-state levels are of physiological significance.

Published
1985

4. [Kinetic model of a multienzyme system of blood prostanoid synthesis. I. Mechanism of stabilization of thromboxane and prostacyclin levels].

Author

Varfolomeev SD, Mevkh AT, and Gachok VP

Subjects
Arachidonic Acid, Arachidonic Acids metabolism, Blood Platelets enzymology, Epoprostenol blood, Humans, Kinetics, Models, Biological, Phospholipases blood, Prostaglandin-Endoperoxide Synthases blood, Substrate Specificity, Thromboxane A2 blood, Epoprostenol biosynthesis, Multienzyme Complexes blood, Thromboxane A2 biosynthesis
Abstract

A kinetic scheme of the prostacyclin-thromboxane system has been evolved on the basis of the authors experimental data and the results described elsewhere. The kinetic behavior of the model has been analysed with the aid of computer technology by varying the following parameters: phospholipase activities, free arachidonic acid exchange rates between platelets and endothelium, PGH-synthetase biosynthesis rates, velocities of arachidonic acid pathways other than the cyclooxygenase ones. It has been demonstrated that the biological system is capable of sustaining prostacyclin and thromboxane concentrations at steady fixed levels within a wide range of kinetic parameters.

Published
1986

5. [Kinetic model of a multienzyme system of blood prostanoid synthesis. II. Dynamic responses to pharmacological agents--inhibitors of prostaglandin H synthetases].

Author

Varfolomeev SD, Mevkh AT, and Gachok VP

Subjects
Arachidonic Acid, Arachidonic Acids blood, Blood Platelets enzymology, Endothelium enzymology, Epoprostenol blood, Humans, Kinetics, Models, Biological, Thromboxane A2 blood, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Cyclooxygenase Inhibitors, Epoprostenol biosynthesis, Multienzyme Complexes blood, Thromboxane A2 biosynthesis
Abstract

The dynamic replies of the multienzyme system of blood prostanoid synthesis to the introduction of an irreversible inhibitor of prostaglandin H synthetase (PGH synthetase) have been analysed by using kinetic modelling. The alterations of arachidonic acid and PGH synthetase concentrations in platelets and endothelium and the concentrations of thromboxane and prostacyclin have been demonstrated. Particularities of kinetic behaviour of the system probably providing the therapeutic effect of non-steroidal anti-inflammatory drugs have been shown. Namely, the kinetic wave of free arachidonic acid and prostacyclin concentration with respect to thromboxane concentration appears after introduction of the drugs.

Published
1986

6. [Kinetic model and mechanism of regulation of a multienzyme system of thromboxane synthesis].

Author

Varfolomeev SD, Gachok VP, and Mevkh AT

Subjects
Arachidonic Acid, In Vitro Techniques, Kinetics, Models, Biological, Arachidonic Acids metabolism, Cyclooxygenase Inhibitors, Multienzyme Complexes metabolism, Phospholipases metabolism, Thromboxanes biosynthesis
Abstract

A kinetic model has been suggested for the thromboxane synthesis polyenzyme system, taking into account the inactivation of a limiting enzyme, prostaglandin H-synthetase, in the course of the reaction. The model also includes the effect of phospholipase, adenylate cyclase, as well as the "outflows" of the components from the system, and basic regulatory effects in the system. A mathematical description of the model is given, and numerical solutions of the system of equations obtained for a wide variety of parameters. An analysis of the kinetic responses of the polyenzyme system shows that: 1) the system is highly conserved with respect to thromboxane concentration changes; 2) two cardinally different modes of the system are registered; they correspond to two insignificantly differing thromboxane steady-state levels; 3) transition of the system from one mode to the other is controlled by the phospholipase activity level which is sensitive to a change in the concentration of the regulator (cyclo-AMP or Ca ions) in the system. It is presumed that the two thromboxane steady-state levels are of physiological significance.

Published
1985

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