1. The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen.
- Author
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Sander, Veronika, Patke, Shantanu, Lee, Jung Y., Young-Tae Chang, and Davidson, Alan J.
- Subjects
DYES & dyeing ,ZEBRA danio ,FISH digestive organs ,SMALL intestine ,EPITHELIAL cells - Abstract
We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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