108 results on '"Liu, Yan"'
Search Results
2. Immunomodulatory Effect of Cordyceps militaris Polysaccharide on RAW 264.7 Macrophages by Regulating MAPK Signaling Pathways.
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Liu, Yan, Yang, Jiayi, Guo, Zhijian, Li, Qizhang, Zhang, Lida, Zhao, Lingxia, and Zhou, Xuanwei
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MITOGEN-activated protein kinases , *BIOACTIVE compounds , *HOT water , *ARABINOSE , *DIETARY supplements , *POLYSACCHARIDES - Abstract
Polysaccharide is one of the principal bioactive components found in medicinal mushrooms and has been proven to enhance host immunity. However, the possible mechanism of immunomodulatory activity of Cordyceps militaris polysaccharide is not fully understood. Hot water extraction and alcohol precipitation, DEAE-Sephadex A-25 chromatography, and Sephadex G-100 chromatography were used to isolate polysaccharide from C. militaris. A high-molecular-weight polysaccharide isolated from C. militaris was designated as HCMP, which had an Mw of 6.18 × 105 Da and was composed of arabinose, galactose, glucose, mannose, and xylose in a mole ratio of 2.00:8.01:72.54:15.98:1.02. The polysaccharide content of HCMP was 91.2% ± 0.16. The test in vitro showed that HCMP activated mouse macrophage RAW 264.7 cells by enhancing phagocytosis and NO production, and by regulating mRNA expressions of inflammation-related molecules in RAW 264.7 cells. Western blotting revealed that HCMP induced the phosphorylation of mitogen-activated protein kinases (MAPKs). Moreover, using inhibitors of MAPKs decreased the mRNA levels of inflammation-related molecules induced by HCMP. These data evidenced that the immunomodulatory effect of HCMP on RAW 264.7 macrophages was mediated via the MAPK signaling pathway. These findings suggested that HCMP could be developed as a potent immunomodulatory agent for use in functional foods and dietary supplements. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Iodophor-Catalyzed Disulfenylation of Amino Naphthalenes with Aryl Sulfonyl Hydrazines.
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Yuan, Yutong, He, Jing, Ma, Xiaowei, Han, Sheng, and Liu, Yan
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HYDRAZINES ,NAPHTHALENE ,BIOCHEMICAL substrates ,SULFIDES ,SULFUR ,HYDRAZINE ,SULFONYL compounds - Abstract
An iodophor-catalyzed direct disulfenylation of amino naphthalenes with aryl sulfonyl hydrazines in water was developed. A series of aryl sulfides were obtained in moderate to excellent yields. The advantages of this green protocol were the simple reaction conditions (metal-free, water as the solvent, under air), the odorless and easily available sulfur reagent, the broad substrate scope, and gram-scale synthesis. Moreover, the potential application of aryl sulfides was exemplified by further transformations. [ABSTRACT FROM AUTHOR]
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- 2024
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4. A 4D-Printable Photocurable Resin Derived from Waste Cooking Oil with Enhanced Tensile Strength.
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Liu, Yan, Liu, Meng-Yu, Fan, Xin-Gang, Wang, Peng-Yu, and Chen, Shuo-Ping
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EDIBLE fats & oils , *TENSILE strength , *SHAPE memory polymers , *SOIL degradation , *RECOVERY rooms , *3-D printers - Abstract
In pursuit of enhancing the mechanical properties, especially the tensile strength, of 4D-printable consumables derived from waste cooking oil (WCO), we initiated the production of acrylate-modified WCO, which encompasses epoxy waste oil methacrylate (EWOMA) and epoxy waste oil acrylate (EWOA). Subsequently, a series of WCO-based 4D-printable photocurable resins were obtained by introducing a suitable diacrylate molecule as the second monomer, coupled with a composite photoinitiator system comprising Irgacure 819 and p-dimethylaminobenzaldehyde (DMAB). These materials were amenable to molding using an LCD light-curing 3D printer. Our findings underscored the pivotal role of triethylene glycol dimethacrylate (TEGDMA) among the array of diacrylate molecules in enhancing the mechanical properties of WCO-based 4D-printable resins. Notably, the 4D-printable material, composed of EWOA and TEGDMA in an equal mass ratio, exhibited nice mechanical strength comparable to that of mainstream petroleum-based 4D-printable materials, boasting a tensile strength of 9.17 MPa and an elongation at break of 15.39%. These figures significantly outperformed the mechanical characteristics of pure EWOA or TEGDMA resins. Furthermore, the EWOA-TEGDMA resin demonstrated impressive thermally induced shape memory performance, enabling deformation and recovery at room temperature and retaining its shape at −60 °C. This resin also demonstrated favorable biodegradability, with an 8.34% weight loss after 45 days of soil degradation. As a result, this 4D-printable photocurable resin derived from WCO holds immense potential for the creation of a wide spectrum of high-performance intelligent devices, brackets, mold, folding structures, and personalized products. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Marine-Derived Bisindoles for Potent Selective Cancer Drug Discovery and Development.
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Xu, Mengwei, Bai, Zhaofang, Xie, Baocheng, Peng, Rui, Du, Ziwei, Liu, Yan, Zhang, Guangshuai, Yan, Si, Xiao, Xiaohe, and Qin, Shuanglin
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DRUG discovery ,MARINE natural products ,DRUG development ,ANTINEOPLASTIC agents ,ACUTE myeloid leukemia ,MARINE toxins - Abstract
Marine-derived bisindoles exhibit structural diversity and exert anti-cancer influence through multiple mechanisms. Comprehensive research has shown that the development success rate of drugs derived from marine natural products is four times higher than that of other natural derivatives. Currently, there are 20 marine-derived drugs used in clinical practice, with 11 of them demonstrating anti-tumor effects. This article provides a thorough review of recent advancements in anti-tumor exploration involving 167 natural marine bisindole products and their derivatives. Not only has enzastaurin entered clinical practice, but there is also a successfully marketed marine-derived bisindole compound called midostaurin that is used for the treatment of acute myeloid leukemia. In summary, investigations into the biological activity and clinical progress of marine-derived bisindoles have revealed their remarkable selectivity, minimal toxicity, and efficacy against various cancer cells. Consequently, they exhibit immense potential in the field of anti-tumor drug development, especially in the field of anti-tumor drug resistance. In the future, these compounds may serve as promising leads in the discovery and development of novel cancer therapeutics. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Establishment and Validation of a Transdermal Drug Delivery System for the Anti-Depressant Drug Citalopram Hydrobromide.
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Sun, Yi-yang, Ni, Ya-jing, Wang, Run-jia, Qin, Zi-cheng, Liu, Zhao, Xiao, Li-hui, and Liu, Yan-qiang
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DRUG delivery systems ,TRANSDERMAL medication ,CITALOPRAM ,METHYLCELLULOSE ,IONTOPHORESIS ,POLYACRYLIC acid ,ANTIDEPRESSANTS - Abstract
To enhance the bioavailability and antihypertensive effect of the anti-depressant drug citalopram hydrobromide (CTH) we developed a sustained-release transdermal delivery system containing CTH. A transdermal diffusion meter was first used to determine the optimal formulation of the CTH transdermal drug delivery system (TDDS). Then, based on the determined formulation, a sustained-release patch was prepared; its physical characteristics, including quality, stickiness, and appearance, were evaluated, and its pharmacokinetics and irritation to the skin were evaluated by applying it to rabbits and rats. The optimal formulation of the CTH TDDS was 49.2% hydroxypropyl methyl cellulose K
100M , 32.8% polyvinylpyrrolidone K30, 16% oleic acid-azone, and 2% polyacrylic acid resin II. The system continuously released an effective dose of CTH for 24 h and significantly enhanced its bioavailability, with a higher area under the curve, good stability, and no skin irritation. The developed CTH TDDS possessed a sustained-release effect and good characteristics and pharmacokinetics; therefore, it has the potential for clinical application as an antidepressant. [ABSTRACT FROM AUTHOR]- Published
- 2024
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7. Determination of Volatile Organic Compounds and Endogenous Extracts and Study of Expression Patterns of TPS and BSMT in the Flowers of Seven Lilium Cultivars.
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Zhang, Peng, Ma, Xiaoou, Zhang, Qian, Guo, Ziyu, Hao, Junyi, Zhang, Zhixuan, Sun, Ming, and Liu, Yan
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VOLATILE organic compounds ,LILIES ,GENE expression ,CULTIVARS ,METHYL benzoate ,FLOWERS ,SOLID phase extraction - Abstract
Lily is one of the most important cut flowers in the world, with a rich floral fragrance. To further explore the fragrance emission mechanisms of lily cultivars, headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS) and organic solvent extraction–gas chromatography–mass spectrometry (OSE-GC-MS) were used to unveil the volatile organic compounds (VOCs) and endogenous extracts of seven lily cultivars. Furthermore, real-time quantitative PCR (qRT-PCR) was used to determine the expression levels of two key genes (TPS and BSMT) related to the biosynthesis of monoterpenoids and methyl benzoate. The results show that forty-five VOCs were detected in the petals of seven lily cultivars, and the main compounds were monoterpenoids and phenylpropanoids/benzenoids. Dichloromethane was the best solvent for extracting the endogenous extracts of Lilium 'Viviana' petals and eighteen endogenous extracts were detected using dichloromethane to extract the petals of seven lily cultivars. Each compound's emission ratio (natural logarithm of the ratio of VOC content to endogenous extract content) was calculated, and linear regression analyses between emission ratios and boiling points were conducted. Significant linear negative correlations existed between the emission ratios and boiling points of compounds, and the regression equations' coefficients of determination (R
2 ) were all greater than 0.7. TPS was expressed highly in 'Viviana', 'Pink News', and 'Palazzo', and BSMT was expressed highly in 'Pink News' and 'Palazzo'. Correlation analyses between the gene expression levels and the monoterpenoids and methyl benzoate contents found that the TPS expression levels have strong positive correlations with monoterpenoids content, while no correlations were found between the expression levels of BSMT and the contents of methyl benzoate. This study lays the foundation for research on the release patterns of VOCs in the flowers of Lilium, and the breeding of lilies for their floral fragrance. [ABSTRACT FROM AUTHOR]- Published
- 2023
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8. Insights on Antitumor Activity and Mechanism of Natural Benzophenanthridine Alkaloids.
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Peng, Rui, Xu, Mengwei, Xie, Baocheng, Min, Qing, Hui, Siwen, Du, Ziwei, Liu, Yan, Yu, Wei, Wang, Shi, Chen, Xin, Yang, Guang, Bai, Zhaofang, Xiao, Xiaohe, and Qin, Shuanglin
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ANTINEOPLASTIC agents ,PHENANTHRIDINE ,ISOQUINOLINE alkaloids ,NATURAL products ,DRUG development ,NEW product development ,STEM cells - Abstract
Benzophenanthridine alkaloids are a class of isoquinoline compounds, which are widely found in the plants of papaveraceae, corydalis, and rutaceae. Biological activities and clinical studies have shown that benzophenanthridine alkaloids have inhibitory effects on many cancers. Considering that the anticancer activities and mechanisms of many natural benzophenanthridine alkaloids have been discovered in succession, the purpose of this paper is to review the anticancer effects of benzophenanthridine alkaloids and explore the application potential of these natural products in the development of antitumor drugs. A literature survey was carried out using Scopus, Pubmed, Reaxys, and Google Scholar databases. This review summarizes and analyzes the current status of research on the antitumor activity and antitumor mechanism of natural products of benzophenanthridine from different sources. The research progress of the antitumor activity of natural products of benzophenanthridine from 1983 to 2023 was reviewed. The antitumor activities of 90 natural products of benzophenanthridine and their related analogues were summarized, and the results directly or indirectly showed that natural products of benzophenanthridine had the effects of antidrug-resistant tumor cell lines, antitumor stem cells, and inducing ferroptosis. In conclusion, benzophenanthridine alkaloids have inhibitory effects on a variety of cancers and have the potential to counteract tumor resistance, and they have great application potential in the development of antitumor drugs. [ABSTRACT FROM AUTHOR]
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- 2023
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9. Synthesis of Spirocyclopropane-Containing 4 H -Pyrazolo[1,5- a ]indoles via Alkylative Dearomatization and Intramolecular N -Imination of an Indole– O -(Methylsulfonyl)oxime.
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Huang, Jiann-Jyh, Liao, Hung-Chun, Hsu, Cheng-En, Liu, Yan-Ru, Chang, Yi-Fu, and Chou, Shan-Yen
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INDOLE compounds ,OXIMES ,HYDROXYLAMINE hydrochloride ,INDOLE - Abstract
In this paper, we report the synthesis of spirocyclopropane-containing 4H-pyrazolo[1,5-a]indoles 6a–e via alkylative dearomatization and intramolecular N-imination of indole–O-(methylsulfonyl)oxime 11. Starting materials tryptophol (7) and 2-bromocyclopetanone (8) were reacted in the presence of HBF
4 ·OEt2 , providing 1,2,3,5,6,11-hexahydrocyclopenta[2,3]oxepino[4,5-b]indole (9) in a 63% yield. Compound 9 was reacted with hydroxylamine hydrochloride to afford oxime 10 (65% yield), which was subsequently bis-methanesulfonated to form 11 in a 85% yield. Heating 11 with various alcohols in the presence of N,N-diisopropylethylamine (DIPEA) triggered the alkylative dearomatization and intramolecular N-imination, forming the spirocyclopropane and 4H-pyrazolo[1,5-a]indole structures in the targets 6a–e with 67–84% yields. [ABSTRACT FROM AUTHOR]- Published
- 2023
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10. Xanthones with Potential Anti-Inflammatory and Anti-HIV Effects from the Stems and Leaves of Cratoxylum cochinchinense.
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Zhang, Yong, Guo, Jia-Ming, Zhang, Ming-Ming, Wang, Ran, Liang, Chai-Huan, Zhao, Yi-Meng, Deng, Ya-Yuan, Liu, Yan-Ping, and Fu, Yan-Hui
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XANTHONE ,ANTI-HIV agents ,HIV ,CHEMICAL structure ,ANTI-inflammatory agents ,LIPOPOLYSACCHARIDES ,NITRIC oxide ,MACROPHAGES - Abstract
Four new xanthones, cratocochinones A–D (1–4), together with eight known analogues (5–12), were isolated from the stems and leaves of Cratoxylum cochinchinense. The chemical structures of cratocochinones A–D (1–4) were elucidated by comprehensive spectroscopic analyses and the known compounds were identified by comparisons with the spectral data reported in the literature. All isolated compounds 1–12 were evaluated for their anti-inflammatory activities and anti-HIV-1 activities. Compounds 1–12 showed remarkable inhibitory activities on nitric oxide (NO) production induced by lipopolysaccharide in mouse macrophage RAW 264.7 cells in vitro, with IC
50 values in the range of 0.86 ± 0.05 to 18.36 ± 0.21 µM. Meanwhile, compounds 1–12 exhibited significant anti-HIV-1 activities with EC50 which ranged from 0.22 to 11.23 µM. These findings indicate that the discoveries of these xanthones, isolated from the stems and leaves of C. cochinchinense, showing significant anti-inflammatory and anti-HIV-1 effects could be of great importance to the research and development of new natural anti-inflammatory and anti-HIV agents. [ABSTRACT FROM AUTHOR]- Published
- 2023
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11. Efficient Fe 3 C-CF Cathode Catalyst Based on the Formation/Decomposition of Li 2−x O 2 for Li-O 2 Batteries.
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Yi, Guanyu, Li, Gaoyang, Jiang, Shuhuai, Zhang, Guoliang, Guo, Liang, Zhang, Xiuqi, Zhao, Zhongkui, Zou, Zhongping, Ma, Hailong, Fu, Xiaojiao, Liu, Yan, and Dang, Feng
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ELECTRIC batteries ,LITHIUM-air batteries ,CATHODES ,CARBON fibers ,DENSITY functional theory ,CATALYTIC activity ,ENERGY density - Abstract
Lithium-oxygen batteries have attracted considerable attention in the past several years due to their ultra-high theoretical energy density. However, there are still many serious issues that must be addressed before considering practical applications, including the sluggish oxygen redox kinetics, the limited capacity far from the theoretical value, and the poor cycle stability. This study proposes a surface modification strategy that can enhance the catalytic activity by loading Fe
3 C particles on carbon fibers, and the microstructure of Fe3 C particle-modified carbon fibers is studied by multiple materials characterization methods. Experiments and density functional theory (DFT) calculations show that the discharge products on the Fe3 C carbon fiber (Fe3 C-CF) cathode are mainly Li2−x O2 . Fe3 C-CF exhibits high catalytic ability based on its promotion of the formation/decomposition processes of Li2−x O2 . Consequently, the well-designed electrode catalyst exhibits a large specific capacity of 17,653.1 mAh g−1 and an excellent cyclability of 263 cycles at a current of 200 mA g−1 . [ABSTRACT FROM AUTHOR]- Published
- 2023
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12. Evaluating the Application Potential of a Recombinant Ganoderma Protein as Bioactive Ingredients in Cosmetics.
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Guo, Zhi-Jian, Liu, Yan, Yang, Jia-Yi, Jin, Meng-Yuan, Mao, Pei-Wen, and Zhou, Xuan-Wei
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MELANOGENESIS , *RECOMBINANT proteins , *MICROPHTHALMIA-associated transcription factor , *FUNGAL proteins , *GANODERMA lucidum , *PICHIA pastoris - Abstract
The aim of this study was to evaluate the application potential of a recombinant fungal immunomodulatory protein from Ganoderma lucidum (rFIP-glu). First, a recombinant plasmid pPIC9K::FIP-glu-His was transferred into Pichia pastoris for the production of protein. The protein was then to assess its free radical scavenging abilities and the effect on the viability of both human immortalized keratinocytes (HaCaT cells) and mouse B16-F10 melanoma cells (B16 cells) in vitro, followed by the effect on the melanin synthesis of B16 cells. The results of SDS-PAGE and western blot showed that rFIP-glu was successfully expressed. Furtherly, a bioactivity assay in vitro indicated that the scavenging rate of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals reached 84.5% at 6.0 mg/mL (p ≤ 0.0001) of rFIP-glu, showing strong antioxidant activity. Subsequently, a safety evaluation demonstrated that rFIP-glu promoted the proliferation of HaCaT cells, with the cell viability reaching 124.3% at 48 μg/mL (p ≤ 0.01), regarding the cell viability of B16 cells after exposure to rFIP-glu (48 μg/mL) significantly inhibited, to 80.7% (p ≤ 0.01). Besides, rFIP-glu inhibited the melanin synthesis of B16 cells in a dose-dependent manner from 100–1000 μg/mL, and rFIP-glu at 500 μg/mL (p ≤ 0.01) exhibited the highest intracellular melanin amount reduction of 16.8%. Furthermore, a mechanism analysis showed that rFIP-glu inhibited tyrosinase (TYR) activity by up-regulating the expression of the microphthalmia-associated transcription factor (MITF) and down-regulating the gene expression of TYR and tyrosinase-related protein-1 (TYRP-1), thus inhibiting melanin synthesis. The data implied that rFIP-glu had significant antioxidant activity and whitening potency. It should be used as raw materials for cosmeceutical applications. [ABSTRACT FROM AUTHOR]
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- 2023
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13. A Comprehensive Review of Natural Flavonoids with Anti-SARS-CoV-2 Activity.
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Yang, Jun-Yu, Ma, Yi-Xuan, Liu, Yan, Peng, Xiang-Jun, and Chen, Xiang-Zhao
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FLAVONOIDS ,COVID-19 treatment ,EPIGALLOCATECHIN gallate ,VACCINE effectiveness ,COVID-19 pandemic - Abstract
The COVID-19 pandemic caused by SARS-CoV-2 has majorly impacted public health and economies worldwide. Although several effective vaccines and drugs are now used to prevent and treat COVID-19, natural products, especially flavonoids, showed great therapeutic potential early in the pandemic and thus attracted particular attention. Quercetin, baicalein, baicalin, EGCG (epigallocatechin gallate), and luteolin are among the most studied flavonoids in this field. Flavonoids can directly or indirectly exert antiviral activities, such as the inhibition of virus invasion and the replication and inhibition of viral proteases. In addition, flavonoids can modulate the levels of interferon and proinflammatory factors. We have reviewed the previously reported relevant literature researching the pharmacological anti-SARS-CoV-2 activity of flavonoids where structures, classifications, synthetic pathways, and pharmacological effects are summarized. There is no doubt that flavonoids have great potential in the treatment of COVID-19. However, most of the current research is still in the theoretical stage. More studies are recommended to evaluate the efficacy and safety of flavonoids against SARS-CoV-2. [ABSTRACT FROM AUTHOR]
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- 2023
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14. Updated Progress of the Copper-Catalyzed Borylative Functionalization of Unsaturated Molecules.
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Li, Bingru, Liang, Huayu, Vignesh, Arumugam, Zhou, Xiaoyu, Liu, Yan, and Ke, Zhuofeng
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COPPER catalysts ,MATERIALS science ,BORYLATION ,ORGANIC synthesis ,COPPER ,ASYMMETRIC synthesis ,PHARMACEUTICAL chemistry - Abstract
Borylation has become a powerful method to synthesize organoboranes as versatile building blocks in organic synthesis, medicinal chemistry, and materials science. Copper-promoted borylation reactions are extremely attractive due to the low cost and non-toxicity of the copper catalyst, mild reaction conditions, good functional group tolerance, and convenience in chiral induction. In this review, we mainly updated recent advances (from 2020 to 2022) in the synthetic transformations in C=C/C≡C multiple bonds, and C=E multiple bonds mediated by copper boryl systems. [ABSTRACT FROM AUTHOR]
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- 2023
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15. GLP-1R Signaling and Functional Molecules in Incretin Therapy.
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Wan, Wenwei, Qin, Qikai, Xie, Linshan, Zhang, Hanqing, Wu, Fan, Stevens, Raymond C., and Liu, Yan
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GLUCAGON-like peptide-1 receptor ,TYPE 2 diabetes ,CELL communication ,MOLECULES ,METABOLIC disorders - Abstract
Glucagon-like peptide-1 receptor (GLP-1R) is a critical therapeutic target for type 2 diabetes mellitus (T2DM). The GLP-1R cellular signaling mechanism relevant to insulin secretion and blood glucose regulation has been extensively studied. Numerous drugs targeting GLP-1R have entered clinical treatment. However, novel functional molecules with reduced side effects and enhanced therapeutic efficacy are still in high demand. In this review, we summarize the basis of GLP-1R cellular signaling, and how it is involved in the treatment of T2DM. We review the functional molecules of incretin therapy in various stages of clinical trials. We also outline the current strategies and emerging techniques that are furthering the development of novel therapeutic drugs for T2DM and other metabolic diseases. [ABSTRACT FROM AUTHOR]
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- 2023
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16. Predictions of Milk Fatty Acid Contents by Mid-Infrared Spectroscopy in Chinese Holstein Cows.
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Zhao, Xiuxin, Song, Yuetong, Zhang, Yuanpei, Cai, Gaozhan, Xue, Guanghui, Liu, Yan, Chen, Kewei, Zhang, Fan, Wang, Kun, Zhang, Miao, Gao, Yundong, Sun, Dongxiao, Wang, Xiao, and Li, Jianbin
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MID-infrared spectroscopy ,MULTIPLE scattering (Physics) ,COWS ,MILK ,DAIRY cattle ,INFRARED spectroscopy - Abstract
Genetic improvement of milk fatty acid content traits in dairy cattle is of great significance. However, chromatography-based methods to measure milk fatty acid content have several disadvantages. Thus, quick and accurate predictions of various milk fatty acid contents based on the mid-infrared spectrum (MIRS) from dairy herd improvement (DHI) data are essential and meaningful to expand the amount of phenotypic data available. In this study, 24 kinds of milk fatty acid concentrations were measured from the milk samples of 336 Holstein cows in Shandong Province, China, using the gas chromatography (GC) technique, which simultaneously produced MIRS values for the prediction of fatty acids. After quantification by the GC technique, milk fatty acid contents expressed as g/100 g of milk (milk-basis) and g/100 g of fat (fat-basis) were processed by five spectral pre-processing algorithms: first-order derivative (DER1), second-order derivative (DER2), multiple scattering correction (MSC), standard normal transform (SNV), and Savitzky–Golsy convolution smoothing (SG), and four regression models: random forest regression (RFR), partial least square regression (PLSR), least absolute shrinkage and selection operator regression (LassoR), and ridge regression (RidgeR). Two ranges of wavebands (4000~400 cm
−1 and 3017~2823 cm−1 /1805~1734 cm−1 ) were also used in the above analysis. The prediction accuracy was evaluated using a 10-fold cross validation procedure, with the ratio of the training set and the test set as 3:1, where the determination coefficient (R2 ) and residual predictive deviation (RPD) were used for evaluations. The results showed that 17 out of 31 milk fatty acids were accurately predicted using MIRS, with RPD values higher than 2 and R2 values higher than 0.75. In addition, 16 out of 31 fatty acids were accurately predicted by RFR, indicating that the ensemble learning model potentially resulted in a higher prediction accuracy. Meanwhile, DER1, DER2 and SG pre-processing algorithms led to high prediction accuracy for most fatty acids. In summary, these results imply that the application of MIRS to predict the fatty acid contents of milk is feasible. [ABSTRACT FROM AUTHOR]- Published
- 2023
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17. Z-Scheme CuO x /Ag/TiO 2 Heterojunction as Promising Photoinduced Anticorrosion and Antifouling Integrated Coating in Seawater.
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Guo, Xiaomin, Pan, Guotao, Fang, Lining, Liu, Yan, and Rui, Zebao
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TITANIUM dioxide ,STERILIZATION (Disinfection) ,REACTIVE oxygen species ,ELECTROLYTIC corrosion ,CORROSION prevention ,SOIL corrosion - Abstract
In the marine environment, steel materials usually encounter serious problems with chemical or electrochemical corrosion and fouling by proteins, bacteria, and other marine organisms. In this work, a green bifunctional Z-scheme CuO
x /Ag/P25 heterostructure coating material was designed to achieve the coordination of corrosion prevention and antifouling by matching the redox potential of the reactive oxygen species and the corrosion potential of 304SS. When CuOx /Ag/P25 heterostructure was coupled with the protected metal, the open circuit potential under illumination negatively shifted about 240 mV (vs. Ag/AgCl) and the photoinduced current density reached 16.6 μA cm−2 . At the same time, more reactive oxygen species were produced by the Z-shape structure, and then the photocatalytic sterilization effect was stronger. Combined with the chemical sterilization of Ag and the oxide of Cu, the bacterial survival rate of CuOx /Ag/P25 was low (0.006%) compared with the blank sample. This design provides a strategy for developing green dual-functional coating materials with photoelectrochemical anticorrosion and antifouling properties. [ABSTRACT FROM AUTHOR]- Published
- 2023
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18. Fabrication and Characterization of β-Cyclodextrin/ Mosla Chinensis Essential Oil Inclusion Complexes: Experimental Design and Molecular Modeling.
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Liu, Hong-Ning, Jiang, Xiao-Xia, Naeem, Abid, Chen, Fu-Cai, Wang, Lu, Liu, Yan-Xia, Li, Zhe, and Ming, Liang-Shan
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ESSENTIAL oils ,INCLUSION compounds ,MOLECULAR dynamics ,EXPERIMENTAL design ,MOLECULAR docking ,CYCLODEXTRINS ,CARVACROL - Abstract
Essential oils (EOs) are primarily isolated from medicinal plants and possess various biological properties. However, their low water solubility and volatility substantially limit their application potential. Therefore, the aim of the current study was to improve the solubility and stability of the Mosla Chinensis (M. Chinensis) EO by forming an inclusion complex (IC) with β-cyclodextrin (β-CD). Furthermore, the IC formation process was investigated using experimental techniques and molecular modeling. The major components of M. Chinensis 'Jiangxiangru' EOs were carvacrol, thymol, o-cymene, and terpinene, and its IC with β-CD were prepared using the ultrasonication method. Multivariable optimization was studied using a Plackett-Burman design (step 1, identifying key parameters) followed by a central composite design for optimization of the parameters (step 2, optimizing the key parameters). SEM, FT-IR, TGA, and dissolution experiments were performed to analyze the physicochemical properties of the ICs. In addition, the interaction between EO and β-CD was further investigated using phase solubility, molecular docking, and molecular simulation studies. The results showed that the optimal encapsulation efficiency and loading capacity of EO in the ICs were 86.17% and 8.92%, respectively. Results of physicochemical properties were different after being encapsulated, indicating that the ICs had been successfully fabricated. Additionally, molecular docking and dynamics simulation showed that β-CD could encapsulate the EO component (carvacrol) via noncovalent interactions. In conclusion, a comprehensive methodology was developed for determining key parameters under multivariate conditions by utilizing two-step optimization experiments to obtain ICs of EO with β-CD. Furthermore, molecular modeling was used to study the mechanisms involved in molecular inclusion complexation. [ABSTRACT FROM AUTHOR]
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- 2023
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19. An Effective Chromatography Process for Simultaneous Purification and Separation of Total Lignans and Flavonoids from Valeriana amurensis.
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Zhang, Manli, Yang, Bingyou, Ye, Minhui, Chen, Jianqing, Liu, Yan, and Wang, Changfu
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LIGNANS ,MACROPOROUS polymers ,VALERIANA ,CHROMATOGRAPHIC analysis ,COLUMN chromatography ,FLAVONOIDS ,PHENOLIC acids ,POLYAMIDES ,NEOLIGNANS - Abstract
An effective chromatography process was developed and validated for simultaneous purification and separation of total lignans and flavonoids from Valeriana amurensis. The total lignans and flavonoids in Valeriana amurensis extract were prepurified with macroporous resin column chromatography, and the conditions were optimized as follows: 40 mg/mL Valeriana amurensis extract (2.0 g) solution was loaded onto an AB-8 resin column with a diameter-to-height ratio of 1:7, followed by adsorption for 6 h; then, the column was eluted successively with 5 BV water and 10% and 50% ethanol at a flow rate 2 BV/h. The obtained 50% ethanol fraction was further repurified and separated by polyamide resin column chromatography to obtain the total lignans and flavonoids, respectively. The chromatography conditions were optimized as follows: a 50% ethanol fraction (1.0 g) was mixed with 1.0 g polyamide resin and loaded onto a polyamide resin (60–100 mesh) column with a diameter-to-height ratio of 1:3; then, the column was eluted successively with 6 BV water and 40% and 80% ethanol at a flow rate of 4 BV/h. The total lignans and flavonoids were obtained from water and 80% ethanol fraction, respectively. The content and recovery of standard compounds in total lignans and flavonoids were analyzed with HPLC-PDA, and the feasibility of the process was confirmed. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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20. A Systematic Method for the Identification of Aporphine Alkaloid Constituents in Sabia schumanniana Diels Using UHPLC-Q-Exactive Orbitrap/Mass Spectrometry.
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E, Shuai, Shang, Zi-Chao, Qin, Shi-han, Li, Kai-lin, Liu, Yan-nan, Wu, Ji-Li, Yan, Fang, and Cai, Wei
- Subjects
MASS spectrometry ,ALKALOIDS ,RECORDS management ,LIQUID chromatography ,RF values (Chromatography) - Abstract
Sabia schumanniana Diels (SSD) is a plant whose stems are used in traditional folk medicine for the treatment of lumbago and arthralgia. Previous studies have revealed chemical constituents of SSD, including triterpenoids and aporphine alkaloids. Aporphine alkaloids contain a variety of active components, which might facilitate the effective treatment of lumbago and arthralgia. However, only 5-oxoaporphine (fuseine) has been discovered in SSD to date. In this study, we sought to systematically identify the aporphine alkaloids in SSD. We established a fast and reliable method for the detection and identification of these aporphine alkaloids based on ultra-high-performance liquid chromatography (UHPLC)-Q-Exactive-Orbitrap/mass spectrometry combined with parallel reaction monitoring (PRM). We separated all of the analyzed samples using a Thermo Scientific Hypersil GOLD™ aQ C18 column (100 mm × 2.1 mm, 1.9 μm). Finally, we identified a total of 70 compounds by using data such as retention times and diagnostic ions. No fewer than 69 of these SSD aporphine alkaloids have been reported here for the first time. These findings may assist in future studies concerning this plant and will ultimately contribute to the research and development of new drugs. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
21. Chinese Cordyceps: Bioactive Components, Antitumor Effects and Underlying Mechanism—A Review.
- Author
-
Liu, Yan, Guo, Zhi-Jian, and Zhou, Xuan-Wei
- Subjects
- *
BIOACTIVE compounds , *CORDYCEPS , *MITOGEN-activated protein kinases , *ADENOSINES , *TUMOR microenvironment - Abstract
Chinese Cordyceps is a valuable source of natural products with various therapeutic effects. It is rich in various active components, of which adenosine, cordycepin and polysaccharides have been confirmed with significant immunomodulatory and antitumor functions. However, the underlying antitumor mechanism remains poorly understood. In this review, we summarized and analyzed the chemical characteristics of the main components and their pharmacological effects and mechanism on immunomodulatory and antitumor functions. The analysis revealed that Chinese Cordyceps promotes immune cells' antitumor function by via upregulating immune responses and downregulating immunosuppression in the tumor microenvironment and resetting the immune cells' phenotype. Moreover, Chinese Cordyceps can inhibit the growth and metastasis of tumor cells by death (including apoptosis and autophagy) induction, cell-cycle arrest, and angiogenesis inhibition. Recent evidence has revealed that the signal pathways of mitogen-activated protein kinases (MAPKs), nuclear factor kappaB (NF-κB), cysteine–aspartic proteases (caspases) and serine/threonine kinase Akt were involved in the antitumor mechanisms. In conclusion, Chinese Cordyceps, one type of magic mushroom, can be potentially developed as immunomodulator and anticancer therapeutic agents. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
22. Inositol Derivatives with Anti-Inflammatory Activity from Leaves of Solanum capsicoides Allioni.
- Author
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Liu, Yan, Meng, Xin, Wang, Han, Sun, Yan, Wang, Si-Yi, Jiang, Yi-Kai, Algradi, Adnan Mohammed, Naseem, Anam, Kuang, Hai-Xue, and Yang, Bing-You
- Subjects
- *
ANTI-inflammatory agents , *INOSITOL , *NORMAL-phase chromatography , *SOLANUM , *SILICA gel , *HIGH performance liquid chromatography - Abstract
Eight new inositol derivatives, solsurinositols A–H (1–8), were isolated from the 70% EtOH extract of the leaves of Solanum capsicoides Allioni. Careful isolation by silica gel column chromatography followed by preparative high-performance liquid chromatography (HPLC) allowed us to obtain analytically pure compounds 1–8. They shared the same relative stereochemistry on the ring but have different acyl groups attached to various hydroxyl groups. This was the first time that inositol derivatives have been isolated from this plant. The chemical structures of compounds 1–8 were characterized by extensive 1D nuclear magnetic resonance (NMR) and 2D NMR and mass analyses. Meanwhile, the in vitro anti-inflammatory activity of all compounds was determined using lipopolysaccharide (LPS)-induced BV2 microglia, and among the isolates, compounds 5 (IC50 = 11.21 ± 0.14 µM) and 7 (IC50 = 14.5 ± 1.22 µM) were shown to have potential anti-inflammatory activity. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
23. Interaction between Heavy Water and Single-Strand DNA: A SERS Study.
- Author
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Jiang, Chengshun, Liu, Yan, Wang, Lianghua, and Lu, Feng
- Subjects
- *
DEUTERIUM oxide , *SERS spectroscopy , *BIOMACROMOLECULES , *DNA , *SINGLE-stranded DNA , *QUADRUPLEX nucleic acids - Abstract
The structure and function of biological macromolecules change due to intermolecular deuterium bond formation or deuterium substitution with environmental D2O. In this study, surface-enhanced Raman spectroscopy (SERS) was used to detect interaction sites between D2O and ssDNA and their action mechanisms. SERS peaks of ssDNA changed with increasing D2O proportions, and the site of action mainly involved A and G bases, whose number strengthened the interaction between sequences and D2O and hence the SERS peak intensities. Fixing the number of A and G bases prevented changes in their positions from significantly altering the map. We also identified the interaction between ssDNA sequences that easily formed a G-quadruplex structure and D2O. The amplitude of the SERS peak intensity change reflected the ssDNA structural stability and number of active sites. These findings are highly significant for exploring genetic exchanges and mutations and could be used to determine the stability and structural changes of biological macromolecules. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
24. Discovery of Novel Potential Aphid Repellents: Geranic Acid Esters Containing Substituted Aromatic Rings.
- Author
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Pan, Shixiang, Li, Wenhao, Qin, Yaoguo, Yang, Zhaokai, Liu, Yan, Shi, Zhuo, Qu, Cheng, Luo, Chen, and Yang, Xinling
- Subjects
PEA aphid ,ODORANT-binding proteins ,APHIDS ,ESTERS ,REPELLENTS - Abstract
Aphids are one of the most damaging agricultural pests. For the sake of novel eco-friendly compounds with good activity for aphid control, a series of novel geranic acid esters containing substituted aromatic rings were designed by inverting ester groups of lead compounds. All compounds were characterized by HRMS,
1 H-NMR, and13 C-NMR. In order to identify the effect of inversion ester groups on activity, a bioassay was conducted. The results showed that the repellent activity against Acyrthosiphon pisum (A. pisum) and the binding affinity with the odorant-binding protein 9 from A. pisum (ApisOBP9) of the compounds were increased after inversion of the ester groups. Particularly, 5f showed the best repellent activity (repellency proportion: 55.6%) and binding affinity (1/Ki: 0.49 µM). Meanwhile, the structure–activity relationships revealed that the introduction of meta-substitution of the benzene ring and halogen atoms, such as Cl and Br, facilitated the biological activity. The further molecular docking results demonstrated that hydrogen bonding interactions and hydrophobic interactions were vital for the binding affinity with ApisOBP9. Additionally, all compounds were predicted to be eco-friendly and their volatile physicochemical properties have been enhanced compared to the leads. The present results provide valuable clues for the further rational design of aphids' behavioral control agents. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
25. Crystal Structure and Noncovalent Interactions of Heterocyclic Energetic Molecules.
- Author
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Liu, Yan, Fan, Jiake, Xue, Zhongqing, Lu, Yajing, Zhao, Jinan, and Hui, Wenyan
- Subjects
- *
CRYSTAL structure , *MOLECULAR shapes , *HETEROCYCLIC compounds , *MOLECULES , *HYDROGEN bonding , *INTERMOLECULAR interactions - Abstract
Nitrogen-rich heterocyclic compounds are important heterocyclic substances with extensive future applications for energetic materials due to their outstanding density and excellent physicochemical properties. However, the weak intermolecular interactions of these compounds are not clear, which severely limits their widespread application. Three nitrogen-rich heterocyclic compounds were chosen to detect their molecular geometry, stacking mode and intermolecular interactions by crystal structure, Hirshfeld surface, RDG and ESP. The results show that all atoms in each molecule are coplanar and that the stacking mode of the three crystals is a planar layer style. A large amount of inter- and intramolecular interaction exists in the three crystals. All principal types of intermolecular contacts in the three crystals are N···H interactions and they account for 40.9%, 38.9% and 32.9%, respectively. Hydrogen bonding, vdW interactions and steric effects in Crystal c are stronger than in Crystals a and b. The negative ESPs all concentrate on the nitrogen atoms in the three molecules. This work is expected to benefit the crystal engineering of heterocyclic energetic materials. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
26. One-Pot Route from Halogenated Amides to Piperidines and Pyrrolidines.
- Author
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Song, Qiao, Wang, Sheng, Lei, Xiangui, Liu, Yan, Wen, Xin, and Wang, Zhouyu
- Subjects
AMIDES ,METAL catalysts ,PIPERIDINE ,POISONS ,PYRROLIDINE - Abstract
Piperidine and pyrrolidine derivatives are important nitrogen heterocyclic structures with a wide range of biological activities. However, reported methods for their construction often face problems of requiring the use of expensive metal catalysts, highly toxic reaction reagents or hazardous reaction conditions. Herein, an efficient route from halogenated amides to piperidines and pyrrolidines was disclosed. In this method, amide activation, reduction of nitrile ions, and intramolecular nucleophilic substitution were integrated in a one-pot reaction. The reaction conditions were mild and no metal catalysts were used. The synthesis of a variety of N-substituted and some C-substituted piperidines and pyrrolidines became convenient, and good yields were obtained. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
27. Designed Syntheses of Three {Ni 6 PW 9 }-Based Polyoxometalates, from Isolated Cluster to Cluster-Organic Helical Chain.
- Author
-
Chen, Chong-An, Liu, Yan, and Yang, Guo-Yu
- Subjects
- *
POLYOXOMETALATES , *SPATIAL arrangement , *STERIC hindrance , *CARBOXYLIC acids , *LIGANDS (Biochemistry) - Abstract
Three new hexa-Ni-substituted Keggin-type polyoxometalates (POMs), [Ni6(OH)3- (DACH)3(H2O)6(PW9O34)]·31H2O (1), [Ni(DACH)2][Ni6(OH)3(DACH)3(HMIP)2(H2O)2(PW9O34)]·56 H2O (2), and [Ni(DACH)2][Ni6(OH)3(DACH)2(AP)(H2O)5(PW9O34)]·2H2O (3) (DACH = 1,2-Diami- nocyclohexane, MIP = 5-Methylisophthalate, AP = Adipate) were successfully made in the presence of DACH under hydrothermal conditions. 1 is an isolated hexa-Ni-substituted Keggin unit decorated by DACH. In order to further construct POM cluster-organic frameworks (POMCOFs) on the basis of 1, by analyzing the steric hindrances and orientations of the POM units, the rigid HMIP and flexible AP ligands were successively incorporated, and another anionic monomeric POM 2 and the new 1D POM cluster organic chain (POMCOC) 3 were obtained. HMIP ligand still acts as a decorating group on the Ni6 core of 2 but results in the different spatial arrangement of the {Ni6PW9} units. AP ligands in 3 successfully bridge adjacent isolated POM cluster units to 1D POMCOC with left-hand helices. The AP in 3 is the longest aliphatic carboxylic acid ligand in POMs, and the 1D POM cluster-AP helical chain represents the first 1D POMCOC with a helical feature. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
28. Noncovalent Interactions and Crystal Structure Prediction of Energetic Materials.
- Author
-
Liu, Yan, An, Chongwei, Liu, Ning, Wang, Minchang, Ye, Baoyun, and Liao, Dongjie
- Subjects
- *
CRYSTAL structure , *CRYSTAL models , *MOLECULAR crystals , *INTERMOLECULAR interactions , *ELECTRIC potential - Abstract
The crystal and molecular structures, intermolecular interactions, and energy of CL-20, HATO, and FOX-7 were comparatively predicted based on molecular dynamic (MD) simulations. By comparison, the 2D fingerprint plot, Hirshfeld surface, reduced density gradient isosurface, and electrostatic potential surface were studied to detect the intermolecular interactions. Meanwhile, the effects of vacuum and different solvents on the crystal habit of CL-20, HATO, and FOX-7 were studied by AE and MAE model, respectively. The energy calculation was also analysed based on the equilibrium structures of these crystal models by MD simulations. Our results would provide fundamental insights for the crystal engineering of energetic materials. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
29. Cloning, Expression Analysis and Functional Characterization of Squalene Synthase (SQS) from Tripterygium wilfordii.
- Author
-
Zhang, Bin, Liu, Yan, Chen, Mengmeng, Feng, Juntao, Ma, Zhiqing, Zhang, Xing, and Zhu, Chuanshu
- Subjects
- *
TRITERPENOIDS , *TRIPTERYGIUM wilfordii , *CHINESE medicine , *MEDICINAL plants , *SQUALENE - Abstract
Celastrol is an active triterpenoid compound derived from Tripterygium wilfordii which is well-known as a traditional Chinese medicinal plant. Squalene synthase has a vital role in condensing two molecules of farnesyl diphosphate to form squalene, a key precursor of triterpenoid biosynthesis. In the present study, T. wilfordii squalene synthase (TwSQS) was cloned followed by prokaryotic expression and functional verification. The open reading frame cDNA of TwSQS was 1242 bp encoding 413 amino acids. Bioinformatic and phylogenetic analysis showed that TwSQS had high homology with other plant SQSs. To obtain soluble protein, the truncated TwSQS without the last 28 amino acids of the carboxy terminus was inductively expressed in Escherichia coli Transetta (DE3). The purified protein was detected by SDS-PAGE and Western blot analysis. Squalene was detected in the product of in vitro reactions by gas chromatograph-mass spectrometry, which meant that TwSQS did have catalytic activity. Organ-specific and inducible expression levels of TwSQS were detected by quantitative real-time PCR. The results indicated that TwSQS was highly expressed in roots, followed by the stems and leaves, and was significantly up-regulated upon MeJA treatment. The identification of TwSQS is important for further studies of celastrol biosynthesis in T. wilfordii. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
30. Pd-Catalyzed, Highly Selective C(sp2)-Br Bond Coupling Reactions of o-(or m-, or p-) Chloromethyl Bromobenzene with Arylboronic Acids.
- Author
-
Pei, Ming-ming, Liu, Ping, Liu, Yan, Lv, Xin-ming, Ma, Xiao-wei, and Dai, Bin
- Subjects
HYDROCARBONS ,ACIDS ,PALLADIUM ,LIGANDS (Biochemistry) ,BIPHENYL compounds ,CHLOROMETHYL group - Abstract
Highly selective C(sp
2 )–C(sp2 ) cross-coupling of dihalogenated hydrocarbons comprising C(sp2 )–Br and C(sp3 )–Cl bonds with arylboronic acids is reported. This highly selective coupling reaction of the C(sp2 )–Br bond is successfully achieved using Pd(OAc)2 and PCy3 ·HBF4 as the palladium source and ligand, respectively. A series of chloromethyl-1,1′-biphenyl compounds are obtained in moderate-to-excellent yields. Moreover, this protocol can be extended to the one-pot dual arylation of 1-bromo-4-(chloromethyl)benzene with two arylboronic acids, leading to diverse unsymmetrical 4-benzyl-1,1′-biphenyl derivatives. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
31. Anti-Fatigue Peptides from the Enzymatic Hydrolysates of Cervus elaphus Blood.
- Author
-
Lv, Jun-Jiang, Liu, Yan, Zeng, Xiao-Yan, Yu, Jia, Li, Yan, Du, Xiao-Qin, Wu, Zhong-Bao, Hao, Shi-Lei, and Wang, Bo-Chu
- Subjects
- *
RED deer , *HIGH performance liquid chromatography , *PEPTIDES , *BIOAVAILABILITY , *AMINO acid metabolism , *AMINO acids - Abstract
Red deer (Cervus elaphus) blood is widely used as a health product. Mixed culture fermentation improves the flavor and bioavailability of deer blood (DB), and both DB and its enzymatic hydrolysates exhibit anti-fatigue activities in vivo. To elucidate the bioactive ingredients, enzymatic hydrolysates were fractioned into different peptide groups using reversed phase resin chromatography, and then evaluated using an exhaustive swimming mice model to assess swimming time and biochemical parameters. The structures of the bioactive peptides were elucidated by high performance liquid chromatography with tandem mass detection. Thirty-one compounds were identified as glutamine or branched-chain amino acids containing short peptides, of which Val-Ala-Asn, Val-Val-Ser-Ala, Leu(Ile)-Leu(Ile)-Val-Thr, Pro-His-Pro-Thr-Thr, Glu-Val-Ala-Phe and Val-Leu(Ile)-Asp-Ala-Phe are new peptides. The fractions containing glutamine or valine short peptides, Ala-Gln, Val-Gln, Val-Val-Ser-Ala, Val-Leu(Ile)-Ser improved exercise endurance by increasing hepatic glycogen (HG) storage. The peptides group containing Leu(Ile)-Leu(Ile), Asp-Gln, Phe- Leu(Ile), Val-Val-Tyr-Pro contributed to decreased muscle lactic acid (MLA)accumulation and to an increase in HG. The anti-fatigue activities of DB hydrolysates were attributed to the synergistic effects of different types of peptides. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
32. Discovery of Active Ingredients Targeted TREM2 by SPR Biosensor-UPLC/MS Recognition System, and Investigating the Mechanism of Anti-Neuroinflammatory Activity on the Lignin-Amides from Datura metel Seeds.
- Author
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Wang, Si-Yi, Liu, Yan, Li, Xiao-Mao, Algradi, Adnan Mohammed, Jiang, Hai, Sun, Yan-Ping, Guan, Wei, Pan, Juan, Kuang, Hai-Xue, and Yang, Bing-You
- Subjects
- *
MYELOID cells , *TUMOR necrosis factors , *TIME-of-flight mass spectrometry , *TAU proteins , *TANDEM mass spectrometry , *NITRIC-oxide synthases - Abstract
As a new target protein for Alzheimer's disease (AD), the triggering receptor expressed on myeloid Cells 2 (TREM2) was expressed on the surface of microglia, which was shown to regulate neuroinflammation, be associated with a variety of neuropathologic, and regarded as a potential indicator for monitoring AD. In this study, a novel recognition system based on surface plasmon resonance (SPR) for the TREM2 target spot was established coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-MS), in order to screen the active ingredients targeting TREM2 from Datura metel seeds. The results showed that four lignan-amides were discovered as candidate compounds by SPR biosensor-UPLC/MS recognition analysis. According to the guidance of the active ingredients discovered by the system, the lignin-amides from Datura metel seeds (LDS) were preliminarily identified as containing 27 lignan-amides, which were enriched compositions by the HP-20 of Datura metel seeds. Meanwhile, the anti-inflammatory activity of LDS was evaluated in BV2 microglia induced by LPS. Our experimental results demonstrated that LDS could reduce NO release in LPS-treated BV2 microglia cells and significantly reduce the expression of the proteins of inducible Nitric Oxide Synthase (iNOS), cyclooxygenase 2 (COX-2), microtubule-associated protein tau (Tau), and ionized calcium-binding adapter molecule 1 (IBA-1). Accordingly, LDS might increase the expression of TREM2/DNAX-activating protein of 12 kDa (DAP12) and suppress the Toll-like receptor SX4 (TLR4) pathway and Recombinant NLR Family, Pyrin Domain Containing Protein 3 (NLRP3)/cysteinyl aspartate specific proteinase-1 (Caspase-1) inflammasome expression by LDS in LPS-induced BV2 microglial cells. Then, the inhibitory release of inflammatory factors Interleukin 1 beta (IL-1β), Interleukin 6 (IL-6), and Tumor necrosis factor-alpha (TNFα) inflammatory cytokines were detected to inhibit neuroinflammatory responses. The present results propose that LDS has potential as an anti-neuroinflammatory agent against microglia-mediated neuroinflammatory disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
33. Immunomodulatory Effects of Green Tea Polyphenols †.
- Author
-
Wang, Shuzhen, Li, Zhiliang, Ma, Yuting, Liu, Yan, Lin, Chi-Chen, Li, Shiming, Zhan, Jianfeng, and Ho, Chi-Tang
- Subjects
EPIGALLOCATECHIN gallate ,GREEN tea ,POLYPHENOLS ,BIOACTIVE compounds ,AUTOIMMUNE diseases ,IMMUNOLOGIC diseases ,TEA - Abstract
Green tea and its bioactive components, especially polyphenols, possess many health-promoting and disease-preventing benefits, especially anti-inflammatory, antioxidant, anticancer, and metabolic modulation effects with multi-target modes of action. However, the effect of tea polyphenols on immune function has not been well studied. Moreover, the underlying cellular and molecular mechanisms mediating immunoregulation are not well understood. This review summarizes the recent studies on the immune-potentiating effects and corresponding mechanisms of tea polyphenols, especially the main components of (–)-epigallocatechin-3-gallate (EGCG) and (–)-epicatechin-3-gallate (ECG). In addition, the benefits towards immune-related diseases, such as autoimmune diseases, cutaneous-related immune diseases, and obesity-related immune diseases, have been discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
34. Solvothermal Synthesis of Multiple Dihydropyrimidinones at a Time as Inhibitors of Eg5.
- Author
-
Jiang, Xiao-Qiang, Chen, Shi-Quan, Liu, Yan-Fei, Pan, Xin-Guang, Chen, Dan, Wang, Shi-Fan, and Dembinski, Roman
- Subjects
MOLECULAR motor proteins ,CELL lines ,LACTIC acid ,HELA cells ,ADENOSINE triphosphatase - Abstract
Solvothermal synthesis of multiple dihydropyrimidinones at a time has been developed in inexpensive and green bio-based solvent lactic acid without any additional catalysts or additives. By this method, thirty new dihydropyrimidinone derivatives were synthesized in two batches and characterized. All of the compounds were screened by Eg5 motor protein ATPase assay, and the positive compounds were tested against the Caco-2 cell line, HeLa cell line, L929 cell line and T24 cell line in vitro. Among them, compound C9 exhibited the best inhibitory activity against motor protein ATPase with an IC
50 value of 30.25 μM and significant cytotoxic activity in the micromolar range against the cells above. The Lineweaver–Burk plot revealed that compound C9 was a mixed-type Eg5 inhibitor. A molecular modeling study using the Discovery Studio program was performed, where compound C9 exhibited good binding interaction with Eg5 motor protein ATPase, and this was consistent with the attained experimental results. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
35. Carrier-Free Immobilization of α-Galactosidase as Nano-Biocatalysts for Synthesizing Prebiotic α-Galacto-Oligosaccharides.
- Author
-
Liu, Yan, Yang, Jingyi, Wang, Ke, Duan, Feiyu, Lu, Lili, and Delattre, Cédric
- Abstract
α-Galacto-oligosaccharides (α-GOSs) have great functions as prebiotics and therapeutics. This work established the method of batch synthesis of α-GOSs by immobilized α-galactosidase for the first time, laying a foundation for industrial applications in the future. The α-galactosidase from Aspergillus niger L63 was immobilized as cross-linked enzyme aggregates (CLEAs) nano-biocatalyst through enzyme precipitating and cross-linking steps without using carriers. Among the tested agents, the ammonium sulfate showed high precipitation efficacy and induced regular structures of α-galactosidase CLEAs (Aga-CLEAs) that had been analyzed by scanning electron microscopy and Fourier-transform infrared spectroscopy. Through optimization by response surface methodology, the ammonium sulfate-induced Aga-CLEAs achieved a high activity recovery of around 90% at 0.55 U/mL of enzymes and 36.43 mM glutaraldehyde with cross-linking for 1.71 h. Aga-CLEAs showed increased thermal stability and organic solvent tolerance. The storage ability was also improved since it maintained 74.5% activity after storing at 4 °C for three months, significantly higher than that of the free enzyme (21.6%). Moreover, Aga-CLEAs exhibited excellent reusability in the α-GOSs synthesis from galactose, retaining above 66% of enzyme activity after 10 batch reactions, with product yields all above 30%. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
36. Unraveling the Roles of Vascular Proteins Using Proteomics.
- Author
-
Liu, Yan, Lin, Tianbao, Valencia, Maria Valderrama, Zhang, Cankui, Lv, Zhiqiang, and Uversky, Vladimir N.
- Subjects
- *
PROTEOMICS , *PROTEINS , *PLANT cells & tissues , *PLANT development , *PLANT defenses , *ABIOTIC stress - Abstract
Vascular bundles play important roles in transporting nutrients, growth signals, amino acids, and proteins between aerial and underground tissues. In order to understand these sophisticated processes, a comprehensive analysis of the roles of the components located in the vascular tissues is required. A great deal of data has been obtained from proteomic analyses of vascular tissues in plants, which mainly aim to identify the proteins moving through the vascular tissues. Here, different aspects of the phloem and xylem proteins are reviewed, including their collection methods, and their main biological roles in growth, and biotic and abiotic stress responses. The study of vascular proteomics shows great potential to contribute to our understanding of the biological mechanisms related to development and defense in plants. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
37. Costunolide Plays an Anti-Neuroinflammation Role in Lipopolysaccharide-Induced BV2 Microglial Activation by Targeting Cyclin-Dependent Kinase 2.
- Author
-
Liu, Yan-Chen, Feng, Na, Li, Wei-Wei, Tu, Peng-Fei, Chen, Jian-Ping, Han, Jing-Yan, and Zeng, Ke-Wu
- Subjects
- *
CYCLIN-dependent kinases , *SURFACE plasmon resonance , *INFLAMMATORY mediators , *PROTEIN structure , *MICROGLIA , *INFLAMMATION , *NITRIC oxide - Abstract
Hyperactivation of microglia in the brain is closely related to neuroinflammation and leads to neuronal dysfunction. Costunolide (CTL) is a natural sesquiterpene lactone with wide pharmacological activities including anti-inflammation and antioxidation. In this study, we found that CTL significantly inhibited the production of inflammatory mediators including nitric oxide, IL-6, TNF-α, and PGE2 in lipopolysaccharide (LPS)-stimulated BV2 microglia. Moreover, CTL effectively attenuated IKKβ/NF-κB signaling pathway activation. To identify direct cellular target of CTL, we performed high-throughput reverse virtual screening assay using scPDB protein structure library, and found cyclin-dependent kinase 2 (CDK2) was the most specific binding protein for CTL. We further confirmed the binding ability of CTL with CDK2 using cellular thermal shift assay (CETSA) and drug affinity responsive target stability (DARTS) assays. Surface plasmon resonance analysis also supported that CTL specifically bound to CDK2 with a dissociation constant at micromole level. Furthermore, knocking down CDK2 obviously reversed the anti-inflammation effect of CTL via AKT/IKKβ/NF-κB signaling pathway on BV-2 cells. Collectively, these results indicate that CTL inhibits microglia-mediated neuroinflammation through directly targeting CDK2, and provide insights into the role of CDK2 as a promising anti-neuroinflammation therapeutic target. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
38. A Heterometallic Three-Dimensional Metal−Organic Framework Bearing an Unprecedented One-Dimensional Branched-Chain Secondary Building Unit.
- Author
-
Chen, Jing, Chao, Meng-Yao, Liu, Yan, Xu, Bo-Wei, Zhang, Wen-Hua, Young, David J., and Gotić, Marijan
- Subjects
METAL-organic frameworks ,METAL ions ,CALCIUM ions ,LIGANDS (Biochemistry) ,CHEMICAL elements - Abstract
A heterometallic metal−organic framework (MOF) of [Cd
6 Ca4 (BTB)6 (HCOO)2 (DEF)2 (H2 O)12 ]∙DEF∙xSol (1, H3 BTB = benzene-1,3,5-tribenzoic acid; DEF = N,N′-diethylformamide; xSol. = undefined solvates within the pore) was prepared by solvothermal reaction of Cd(NO3 )2 ·4H2 O, CaO and H3 BTB in a mixed solvent of DEF/H2 O/HNO3 . The compatibility of these two divalent cations from different blocks of the periodic table results in a solid-state structure consisting of an unusual combination of a discrete V-shaped heptanuclear cluster of [Cd2 Ca]2 Ca′ and an infinite one-dimensional (1D) chain of [Cd2 CaCa′]n that are orthogonally linked via a corner-shared Ca2+ ion (denoted as Ca′), giving rise to an unprecedented branched-chain secondary building unit (SBU). These SBUs propagate via tridentate BTB to yield a three-dimensional (3D) structure featuring a corner-truncated P41 helix in MOF 1. This outcome highlights the unique topologies possible via the combination of carefully chosen s- and d-block metal ions with polydentate ligands. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF
39. UPLC-MS/MS Identification and Quantification of Withanolides from Six Parts of the Medicinal Plant Datura Metel L.
- Author
-
Yang, Shi Hui, Liu, Yan, Wang, Qi, Sun, Yan Ping, Guan, Wei, Liu, Yuan, Yang, Bing You, and Kuang, Hai Xue
- Subjects
- *
WITHANOLIDES , *MEDICINAL plants , *QUALITY control , *IDENTIFICATION , *QUANTITATIVE research - Abstract
Withanolides from six parts (flower, leaf, stem, root, seed, and peel) of Datura metel L. (D metel L.) obtained from ten production areas in China were identified and quantified by UPLC-MS/MS. A total of 85 withanolides were characterized for the first time using the UPLC-Q-TOF-MS/MS system. Additionally, a simultaneous, rapid and accurate measurement method was developed for the determination of 22 bioactive withanolides from ten production areas with the UPLC-Q-TRAP-MS/MS system. The results show the total withanolide content is highest in the leaves (155640.0 ng/g) and lowest in the roots (14839.8 ng/g). Compared with other production areas, the total content of plants from Dujiangyan was the highest at 82013.9 ng/g (value range of ten areas: 82013.9–42278.5 ng/g). The results also show significant differences in the distribution of withanolides in the different plant parts, as well as across different production areas. This is a breakthrough report providing a simultaneous qualitative and quantitative analysis of 22 withanolides in D. metel L. It could be the basis for the more rational use of various parts of D. metel L., and the expansion of medicinal resources. This work also lays a solid foundation for research on the quality control of D. metel L. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
40. Amino Acid-Linked Low Molecular Weight Polyethylenimine for Improved Gene Delivery and Biocompatibility.
- Author
-
Wu, Xiao-Ru, Zhang, Ji, Zhang, Ju-Hui, Xiao, Ya-Ping, He, Xi, Liu, Yan-Hong, and Yu, Xiao-Qi
- Subjects
MOLECULAR weights ,POLYETHYLENEIMINE ,HISTIDINE ,GEL permeation chromatography ,GENES ,BIOCOMPATIBILITY ,GENE therapy - Abstract
The construction of efficient and low toxic non-viral gene delivery vectors is of great significance for gene therapy. Herein, two novel polycations were constructed via Michael addition from low molecular weight polyethylenimine (PEI) 600 Da and amino acid-containing linkages. Lysine and histidine were introduced for the purpose of improved DNA binding and pH buffering capacity, respectively. The ester bonds afforded the polymer biodegradability, which was confirmed by the gel permeation chromatography (GPC) measurement. The polymers could well condense DNA into nanoparticles and protect DNA from degradation by nuclease. Compared with PEI 25 kDa, these polymers showed higher transfection efficiency, lower toxicity, and better serum tolerance. Study of this mechanism revealed that the polyplexes enter the cells mainly through caveolae-mediated endocytosis pathway; this, together with their biodegradability, facilitates the internalization of polyplexes and the release of DNA. The results reveal that the amino acid-linked low molecular weight PEI polymers could serve as promising candidates for non-viral gene delivery. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
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41. The Underlying Mechanisms of Curcumin Inhibition of Hyperglycemia and Hyperlipidemia in Rats Fed a High-Fat Diet Combined With STZ Treatment.
- Author
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Xia, Zhen-Hong, Chen, Wen-Bo, Shi, Li, Jiang, Xue, Li, Ke, Wang, Yu-Xiang, and Liu, Yan-Qiang
- Subjects
HIGH-fat diet ,HYPERGLYCEMIA ,ASPARTATE aminotransferase ,CURCUMIN ,HYPERLIPIDEMIA ,TURMERIC ,BLOOD sugar - Abstract
Curcumin is the main secondary metabolite of Curcuma longa and other Curcuma spp, and has been reported to have some potential in preventing and treating some physiological disorders. This study investigated the effect of curcumin in inhibiting high-fat diet and streptozotocin (STZ)-induced hyperglycemia and hyperlipidemia in rats. Twenty-six male Sprague-Dawley (SD) rats (170–190 g) were randomly divided into a standard food pellet diet group (Control group), a high-fat diet and streptozotocin group (HF + STZ group), and a high-fat diet combined with curcumin and STZ group (HF + Cur + STZ group). Compared with the HF + STZ group, the HF + Cur + STZ group exhibited significantly reduced fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (AST), and aspartate transaminase (ALT) levels, as well as liver coefficients. In the livers of these rats, the expression of malondialdehyde (MDA) and Bax was downregulated, whereas that of superoxide dismutase (SOD) and Bcl-2 was upregulated. Moreover, the liver histology of these rats was improved and resembled that of the control rats. These results suggest that curcumin prevents high-fat diet and STZ-induced hyperglycemia and hyperlipidemia, mainly via anti-oxidant and anti-apoptotic mechanisms in the liver. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
42. Inhibitory Effect of Osthole from Cnidium monnieri on Tobacco Mosaic Virus (TMV) Infection in Nicotiana glutinosa.
- Author
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Chen, Ya-Han, Guo, Dong-Sheng, Lu, Mei-Huan, Yue, Jian-Ying, Liu, Yan, Shang, Chun-Ming, An, De-Rong, Zhao, Ming-Min, and Capasso, Raffaele
- Subjects
TOBACCO mosaic virus ,BACOPA monnieri ,NICOTIANA ,BIOLOGICAL reagents ,INFECTION ,PHYSIOLOGICAL control systems - Abstract
The coumarin compound of osthole was extracted from Cnidium monnieri and identified by LC-MS and
1 H- and13 C-NMR. Osthole was tested for anti-virus activity against tobacco mosaic virus (TMV) using the half-leaf method. The results showed that stronger antiviral activity on TMV infection appeared in Nicotiana glutinosa than that of eugenol and ningnanmycin, with inhibitory, protective, and curative effects of 72.57%, 70.26%, and 61.97%, respectively. Through observation of the TMV particles, we found that osthole could directly affect the viral particles. Correspondingly, the level of coat protein detected by Western blot was significantly reduced when the concentrations of osthole increased in tested plants compared to that of the control. These results suggest that osthole has anti-TMV activity and may be used as a biological reagent to control the plant virus in the half-leaf method. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF
43. Multi-Evaluating Strategy for Siji-kangbingdu Mixture: Chemical Profiling, Fingerprint Characterization, and Quantitative Analysis.
- Author
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Yao, Zhuoru, Yu, Jingao, Tang, Zhishu, Liu, Hongbo, Ruan, Kaihua, Song, Zhongxing, Liu, Yanru, Yan, Kun, Liu, Yan, Tang, Yuping, and Ma, Huqiang
- Subjects
STANDARDIZATION ,RESPIRATORY infections ,QUANTITATIVE research ,PHENOL content of food ,CHINESE medicine ,CHLOROGENIC acid - Abstract
Siji-kangbingdu mixture is an anti-inflammatory, anti-bacterial, and anti-viral herbal mixture which is frequently used by doctors to treat upper respiratory infections. It's important to establish an efficient and economical quality-control method to ensure the quality consistency and efficacy stability of Siji-kangbingdu mixture. In this study, an integrated multi-evaluation method was established, sequentially involving UPLC-TripleTOF-MS analysis, UPLC fingerprint analysis, and the quantitative analysis of multi-components using the single-marker (QAMS) method. With one chromatographic condition, a total of 71 compounds were identified by MS and MS/MS information, with a mass error of less than 5 ppm; 49 peaks detected in 254 nm were selected to establish the fingerprint similarity model, and 7 chemical compounds were simultaneously determined, namely, chlorogenic acid, liquiritin, rutin, isochlorogenic acid A, forsythin, forsythoside A, and glycyrrhizic acid, with forsythoside A as the reference standard. There was no significant difference in the content of the seven compounds between the QAMS method and the external standard method (ESM). The established multi-evaluation method will largely promote the quality control and standardization process of Siji-kangbingdu mixture. It also provides a reference workflow for the overall evaluation of TCM patent medicines, from chemical profiling to fingerprint and quantitative analysis. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
44. Protective Responses Induced by Chiral 3-Dichloroacetyl Oxazolidine Safeners in Maize (Zea mays L.) and the Detoxification Mechanism.
- Author
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Gao, Shuang, Liu, Yan-Yan, Jiang, Jing-Yu, Fu, Ying, Zhao, Li-Xia, Li, Chun-Yan, and Ye, Fei
- Subjects
- *
ACETOLACTATE synthase , *OPTICAL isomers , *HERBICIDES , *PHYTOTOXICITY , *MOLECULAR docking , *CORN - Abstract
Herbicide safeners selectively protect crops from herbicide injury while maintaining the herbicidal effect on the target weed. To some extent, the detoxification of herbicides is related to the effect of herbicide safeners on the level and activity of herbicide target enzymes. In this work, the expression of the detoxifying enzyme glutathione S-transferase (GST) and antioxidant enzyme activities in maize seedlings were studied in the presence of three potential herbicide safeners: 3-dichloroacetyl oxazolidine and its two optical isomers. Further, the protective effect of chiral herbicide safeners on detoxifying chlorsulfuron in maize was evaluated. All safeners increased the expression levels of herbicide detoxifying enzymes, including GST, catalase (CAT), and peroxidase (POD) to reduce sulfonylurea herbicide phytotoxicity in maize seedlings. Our results indicate that the R-isomer of 3-(dichloroacetyl)-2,2,5-trimethyl-1,3-oxazolidine can induce glutathione (GSH) production, GST activity, and the ability of GST to react with the substrate 1-chloro-2,4-dinitrobenzene (CDNB) in maize, meaning that the R-isomer can protect maize from damage by chlorsulfuron. Information about antioxidative enzyme activity was obtained to determine the role of chiral safeners in overcoming the oxidative stress in maize attributed to herbicides. The interaction of safeners and active target sites of acetolactate synthase (ALS) was demonstrated by molecular docking modeling, which indicated that both isomers could form a good interaction with ALS. Our findings suggest that the detoxification mechanism of chiral safeners might involve the induction of the activity of herbicide detoxifying enzymes as well as the completion of the target active site between the safener and chlorsulfuron. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
45. Eosin Removal by Cetyl Trimethylammonium-Cloisites: Influence of the Surfactant Solution Type and Regeneration Properties.
- Author
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Kooli, Fethi, Rakass, Souad, Liu, Yan, Abboudi, Mostafa, Oudghiri Hassani, Hicham, Muhammad Ibrahim, Sheikh, Al Wadaani, Fahd, and Al-Faze, Rawan
- Subjects
SURFACE active agents ,SOLUTION (Chemistry) ,ORGANOCLAY ,CLAY minerals ,CLAY ,EOSIN - Abstract
The effect of the counteranion of hexadecyltrimethylammonium salts on the physico-chemical properties of organoclays was investigated, using a selected natural clay mineral with a cation exchange capacity of 95 meq/100 g. The uptake amount of C
16 cations was dependent on the hexadecyltrimethylammonium (C16 ) salt solution used, the organoclay prepared from C16 Br salt solution exhibited a value of 1. 05 mmole/g higher than those prepared from C16 Cl and C16 OH salt solutions. The basal spacing of these organoclays was in the range of 1.81 nm to 2.10 nm, indicating a similar orientation of the intercalated surfactants, and could indicated that the excess amount of surfactants, above the cation exchange capacity of 0.95 meq/g could be adsorbed on the external surface of the clay mineral sheets. These organoclays were found to be stable in neutral, acidic, and basic media. The thermal stability of these organoclays was carried out using thermogravimetric analysis and in-situ X-ray diffraction (XRD) techniques. The decomposition of the surfactant occurred at a maximum temperature of 240 °C, accompanied with a decrease of the basal spacing value close to 1.42 nm. The application of these organoclays was investigated to remove an acidic dye, eosin. The removal amount was related to the initial used concentrations, the amount of the surfactants contents, and to the preheated temperatures of the organoclays. The removal was found to be endothermic process with a maximum amount of 55 mg of eosin/g of organoclay. The value decreased to 25 mg/g, when the intercalated surfactants were decomposed. The reuse of these organoclays was limited to four regeneration recycles with a reduction of 20 to 30%. However, noticeable reduction between 35% to 50% of the initial efficiency, was achieved after the fifth cycle, depending of the used organoclays. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
46. Datura Metel L. Ameliorates Imiquimod-Induced Psoriasis-Like Dermatitis and Inhibits Inflammatory Cytokines Production through TLR7/8–MyD88–NF-κB–NLRP3 Inflammasome Pathway.
- Author
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Yang, Bing-You, Cheng, Yan-Gang, Liu, Yan, Liu, Yuan, Tan, Jin-Yan, Guan, Wei, Guo, Shuang, and Kuang, Hai-Xue
- Subjects
MYELOID differentiation factor 88 ,CD54 antigen ,ENZYME-linked immunosorbent assay ,SKIN inflammation ,CHINESE medicine ,CYTOKINES - Abstract
Background: Psoriasis is a chronic, immune-mediated inflammatory skin disease, and the inflammatory response plays an important role in its development and progression. Datura metel L. is a traditional Chinese medicine that exhibited a significant therapeutic effect on psoriasis in our previous study due to its remarkable anti-inflammatory effect. Meanwhile, the mechanism underlying its effects on psoriasis is still unclear. Methods: An imiquimod-induced psoriasis-like dermatitis mouse model was constructed to evaluate the protective effect of the effective part of Datura metel L. (EPD), which was verified by evaluations of the Psoriasis Area and Severity Index (PASI) score. Hematoxylin and eosin (H&E) staining, immunohistochemical examination, enzyme-linked immunosorbent assay (ELISA), and Western blot were used to measure the inflammatory cytokines and the protein expression associated with the Toll-like receptor 7– myeloid differentiation primary response gene 88–nuclear Factor-κB–nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (TLR7/8–MyD88–NF-κB–NLRP3) inflammasome pathway. Results: EPD significantly decreased the PASI, reduced epidermal thickness, and decreased the proliferation and differentiation of epidermal cells in psoriasis-like dermatitis C57BL/6 mice induced by imiquimod (IMQ). Furthermore, EPD reduced the infiltration of CD3+ cells to psoriatic lesions, as well as ameliorated the elevations of intercellular adhesion molecule 1 (ICAM-1) and inhibited the production of imiquimod-induced inflammatory cytokines, including IL-1β, IL-2, IL-6, IL-10, IL-12, IL-17, IL-22, IL-23, tumor necrosis factor-α (TNF-α), monocyte chemotactic protein 1 (MCP-1), and interferon-γ (IFN-γ). Besides, EPD decreased the imiquimod-induced expression levels of TLR7, TLR8, TRAF6, MyD88, p-IKKα, p-IKBα, p-NF-κB, NLRP3, apoptosis-associated speck-like protein contained a caspase recruitment domain (ASC), cysteinyl aspartate specific proteinase 1 (caspase-1), and IL-1β. Conclusion: This study demonstrated that EPD exhibited a protective effect on an imiquimod-induced psoriasis mice model by inhibiting the inflammatory response, which might be ascribed to the inhibition of the TLR7/8–MyD88–NF-κb–NLRP3 inflammasome pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
47. The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells.
- Author
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Lin, Li, Liu, Yan-Chun, Liu, Zhao-Ying, Epifano, Francesco, and Fiorito, Serena
- Abstract
Two optical isomers, +/− gelsemine (1, 2), together with one known compound were isolated from the whole plant of G. elegans. The structures of the separated constituents were elucidated on 1D and 2D (1H-1H COSY, HMBC, HSQC) NMR spectroscopy and high-resolution mass spectrometry (HRMS). The isolated alkaloids were tested in vitro for cytotoxic potential against PC12 cells by the MTT assay. As a result, (+) gelsemine (compound 1) exhibited cytotoxic activity against PC12 cells with an IC50 value of 31.59 μM, while (−) gelsemine (compound 2) was not cytotoxic. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
48. Proteomics Research on the Protective Effect of Mangiferin on H9C2 Cell Injury Induced by H2O2.
- Author
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Guan, Wei, Liu, Yan, Liu, Yuan, Wang, Qi, Ye, Hong-Liang, Cheng, Yan-Gang, Kuang, Hai-Xue, Jiang, Xi-Cheng, and Yang, Bing-You
- Abstract
Cardiovascular disease is one of the leading causes of morbidity and mortality worldwide. Mangiferin is a natural glucosylxanthone with antioxidant and anti-inflammatory properties, which has been confirmed to protect cardiac cells from myocardial infarction and myocardial ischemia reperfusion injury (MIRI); however, the underlying mechanism is still unclear. As oxidative stress is a major pathogenesis of MIRI, an H9C2 cell injury induced by hydrogen peroxide (H2O2) was established to simulate MIRI in vitro. Herein, the protective effect of mangiferin against MIRI was evaluated and the isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics was applied to explore the underlying molecular mechanism. In this research, mangiferin markedly ameliorated the oxidative imbalance by increasing the antioxidative capacity of the H9C2 cell. Moreover, proteomics analysis revealed that mangiferin pretreatment brought twenty differently-expressed proteins back to normal, most of which were related to glucose and fatty acid metabolism. Glycolysis, citrate cycle, and fatty acid degradation pathways were highlighted by Kyoto Encyclopedia of Gene and Genomes (KEGG) analysis. Western blot validation of six cardiac metabolism-related proteins were consistent with the proteomics analysis. Taken together, mangiferin protected the cardiomyocytes from MIRI by enhancing the antioxidant capacity and increasing the activities of glycolysis, citrate cycle, and fatty acid degradation pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
49. Ultrasonic Processing Induced Activity and Structural Changes of Polyphenol Oxidase in Orange (Citrus sinensis Osbeck).
- Author
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Zhu, Lijuan, Zhu, Linhu, Murtaza, Ayesha, Liu, Yan, Liu, Siyu, Li, Junjie, Iqbal, Aamir, Xu, Xiaoyun, Pan, Siyi, Hu, Wanfeng, and Serra, Stefano
- Abstract
Apart from non-enzymatic browning, polyphenol oxidase (PPO) also plays a role in the browning reaction of orange (Citrus sinensis Osbeck) juice, and needs to be inactivated during the processing. In this study, the protein with high PPO activity was purified from orange (Citrus sinensis Osbeck) and inactivated by ultrasonic processing. Fluorescence spectroscopy, circular dichroism (CD) and Dynamic light scattering (DLS) were used to investigate the ultrasonic effect on PPO activity and structural changes on purified PPO. DLS analysis illustrated that ultrasonic processing leads to initial dissociation and final aggregation of the protein. Fluorescence spectroscopy analysis showed the decrease in fluorescence intensity leading to the exposure of Trp residues to the polar environment, thereby causing the disruption of the tertiary structure after ultrasonic processing. Loss of α-helix conformation leading to the reorganization of secondary structure was triggered after the ultrasonic processing, according to CD analysis. Ultrasonic processing could induce aggregation and modification in the tertiary and secondary structure of a protein containing high PPO activity in orange (Citrus sinensis Osbeck), thereby causing inactivation of the enzyme. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
50. Sample Digestion and Combined Preconcentration Methods for the Determination of Ultra-Low Gold Levels in Rocks.
- Author
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Liu, Yan-hong, Wan, Bo, Xue, Ding-shuai, and Neng, Nuno
- Subjects
- *
GOLD nanoparticles , *INDUCTIVELY coupled plasma mass spectrometry , *NANOPARTICLES , *ADSORPTION (Chemistry) , *MASS spectrometry - Abstract
The gold abundance in basic rocks, which normally varies between 0.5 and 5 ppb, has served as a very important indicator in many geoscience studies, including those focused on the planetary differentiation, redistribution of elements during the crustal process, and ore genesis. However, because gold is a monoisotopic element that exhibits a nugget effect, it is very difficult to quantify its ultra-low levels in rocks, which significantly limits our understanding of the origin of gold and its circulation between the Earth crust, mantle, and core. In this work, we summarize various sample digestion and combined preconcentration methods for the determination of gold amounts in rocks. They include fire assay, fire assay combined with Te coprecipitation and instrumental neutron activation analysis (INAA) or laser ablation inductively coupled plasma mass spectrometry, fusion combined with Te coprecipitation and anion exchange resins, dry chlorination, wet acid digestion combined with precipitation, ion exchange resins, solvent extraction, polyurethane foam, extraction chromatography, novel solid adsorbents, and direct determination by INAA. In addition, the faced challenges and future perspectives in this field are discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
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