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Your search keyword '"Khan, Amir"' showing total 22 results
Start Over Author "Khan, Amir" Journal molecular therapy
22 results on '"Khan, Amir"'

3. 505. VGX-3100 Drives Regression of HPV16/18 CIN2/3 and Robust Cellular Immune Responses in Blood and Cervical Tissue in a Blinded, Randomized, Placebo-Controlled Phase 2B Study

Author

Morrow, Matthew, primary, Trimble, Connie, additional, Shen, Xuefei, additional, Dallas, Michael, additional, Weiner, David, additional, Boyer, Jean, additional, Yan, Jian, additional, Kraynyak, Kimberly, additional, Sylester, Albert, additional, Giffear, Mary, additional, Marcozzi-Pierce, Kathleen, additional, Shah, Divya, additional, Broderick, Kate, additional, Khan, Amir, additional, Lee, Jessica, additional, Humeau, Laurent, additional, Sardesai, Niranjan, additional, and Bagarazzi, Mark, additional

Published
2016
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4. 479. An Optimized DNA Vaccine Formulation Protects Against Lethal Ebola Makona Virus Challenge in Non-Human Primates and Elicits Robust Immune Responses

Author

Patel, Ami, primary, Reuschel, Emma L., additional, Kraynyak, Kimberly A., additional, Racine, Trina, additional, Park, Daniel H., additional, Keaton, Amelia A., additional, Karuppiah, Muthumani, additional, Shedlock, Devon, additional, Yan, Jian, additional, Khan, Amir, additional, Tierney, Kevin, additional, Sardesai, Niranjan, additional, Kobinger, Gary P., additional, and Weiner, David B., additional

Published
2016
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5. 436. Developing a Synthetic DNA Vaccine for an Emerging Pathogen - Middle East Respiratory Syndrome

Author

Reuschel, Emma L., primary, Muthumani, Karrupiah, additional, Kraynyak, Kim, additional, Tingey, Colleen, additional, Kudchodkar, Sagar B., additional, Khan, Amir S., additional, Sardesai, Niranjan Y., additional, Kim, J. Joseph, additional, Maslow, Joel, additional, Park, Young K., additional, Kobinger, Gary, additional, Falzarano, Darryl, additional, Feldman, Heinrich, additional, and Weiner, David B., additional

Published
2016
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6. 450. A Single Immunization With Optimized DNA Vaccines Protects Against Lethal Ebola Virus Challenge in Mice and Induces Seroconversion in Non-Human Primates Following a DNA Prime–DNA Boost Approach

Author

Patel, Ami, primary, Reuschel, Emma L., additional, Scott, Veronica L., additional, Kraynyak, Kimberly A., additional, Wong, Gary, additional, Villarreal, Daniel O., additional, Karuppiah, Muthumani, additional, Shedlock, Devon, additional, Yan, Jian, additional, Broderick, Kate E., additional, Khan, Amir, additional, Tierney, Kevin, additional, Kobinger, Gary P., additional, Sardesai, Niranjan, additional, and Weiner, David B., additional

Published
2015
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10. Synthetic Consensus HIV-1 DNA Induces Potent Cellular Immune Responses and Synthesis of Granzyme B, Perforin in HIV Infected Individuals

Author

Morrow, Matthew P, primary, Tebas, Pablo, additional, Yan, Jian, additional, Ramirez, Lorenzo, additional, Slager, Anna, additional, Kraynyak, Kim, additional, Diehl, Malissa, additional, Shah, Divya, additional, Khan, Amir, additional, Lee, Jessica, additional, Boyer, Jean, additional, Kim, J Joseph, additional, Sardesai, Niranjan Y, additional, Weiner, David B, additional, and Bagarazzi, Mark L, additional

Published
2015
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13. Comparative Analysis of Immune Responses Induced by Vaccination With SIV Antigens by Recombinant Ad5 Vector or Plasmid DNA in Rhesus Macaques

Author

Hirao, Lauren A, primary, Wu, Ling, additional, Satishchandran, Abhishek, additional, Khan, Amir S, additional, Draghia-Akli, Ruxandra, additional, Finnefrock, Adam C, additional, Bett, Andrew J, additional, Betts, Michael R, additional, Casimiro, Danilo R, additional, Sardesai, Niranjan Y, additional, Kim, J Joseph, additional, Shiver, John W, additional, and Weiner, David B, additional

Published
2010
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20. 190. Improvement of Electroporation Devices and Methods for Use with Plasmid-Based Therapeutics and DNA Vaccine Applications in Small and Large Animals

Author

Khan, Amir S. and Draghia-Akli, Ruxandra

Subjects
*DNA vaccines, *ELECTROPORATION
Abstract

An abstract of the article "Improvement of Electroporation Devices and Methods for Use with Plasmid-Based Therapeutics and DNA Vaccine Applications in Small and Large Animals," by Amir S. Khan and Ruxandra Draghia-Akli is presented.

Published
2005
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21. 598. Effective Humoral and Cellular Smallpox-Immune Responses Using a Combination of Plasmids Delivered by Intramuscular Injection Followed by Constant-Current Electroporation in Rabbits.

Author

S. Khan, Amir, Hirao, Lauren, Weiner, David B., and Draghia-Akli, Ruxandra

Subjects
*SMALLPOX, *INTRAMUSCULAR injections, *ENZYME-linked immunosorbent assay, *LABORATORY rabbits, *IMMUNOGLOBULINS
Abstract

Previous studies have examined smallpox antigen-expressing plasmids in mice and non-human primates with limited efficacy. Recent studies showed that the electroporation technique can be used to dramatically increase plasmid uptake into the target tissues (e.g., skeletal muscle) when compared to simple injection. Consequently, the expression levels for the encoded transgene are orders of magnitude higher, likely improving potency. In a pilot experiment, uptake and distribution of plasmids with and without electroporation have been analyzed in the rabbit. Electroporated muscle showed a better distribution and uptake. We evaluated immune responses to smallpox antigens, after direct intramuscular plasmid injection followed by constant-current electroporation in groups of rabbits (n=3/group). The plasmids were administered either individually or in combination and their relative responses analyzed. Group 1 rabbits were immunized with a combination of eight plasmids expressing varying smallpox antigens (A13L, A14L, A27L, A33L, B5R, D8L, H3L, L1R). Group 2 rabbits were immunized with a combination of four different antigens (A27L, B5R, D8L, L1R). The final group received an immunization with an individual plasmid expressing a single antigen (B5R). All plasmids were administered via a single IM injection of 800 μg (100 μg each antigen and/or empty vector up to 800 μg per injection) into semimembranosus muscle followed by electroporation using the ADViSYS constant-current EKD system, at 0.6 Amps, 3 pulses, 52 ms/pulse, 1 sec between pulses. Rabbits were immunized twice, at Study d 0 and at Study d 64. Sera were collected from rabbits at different time points, including on Day 85 and used to determine antibody responses by protein ELISA. Antibody responses were improved by electroporation in both immunized groups irrespective of the number of antigens delivered. A specific ELISpot assay for TNF is being developed to determine T-cell response rates in rabbits following DNA immunization. Rabbit PBMCs collected 2 weeks after first immunization were stimulated for 24h with 15mer peptides that span the entire length of the A27L gene. Immunization followed by electroporation induced improved antigen specific T-cell responses against A27L. Thus, the combination immunization strategy using smallpox antigens and constant-current electroporation appears important for driving better humoral and cellular-based responses in a relevant animal model.Molecular Therapy (2006) 13, S231–S231; doi: 10.1016/j.ymthe.2006.08.672 [ABSTRACT FROM AUTHOR]

Published
2006
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