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Your search keyword '"Bovolenta, Chiara"' showing total 11 results
Start Over Author "Bovolenta, Chiara" Journal molecular therapy
11 results on '"Bovolenta, Chiara"'

3. 130. Purification of Large Scale mRNA Encoding ZFN Nucleases by dHPLC Technology

Author

Giuliani, Erica, primary, Piovan, Claudia, additional, Bossi, Sergio, additional, Corna, Stefano, additional, Scavullo, Cinzia, additional, Pema, Monika, additional, Di Tomaso, Tiziano, additional, Genovese, Pietro, additional, Lombardo, Angelo, additional, Naldini, Luigi, additional, Bordignon, Claudio, additional, Rizzardi, Gian Paolo, additional, and Bovolenta, Chiara, additional

Published
2016
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4. 37. Towards Clinical Translation of Hematopoietic Stem Cell Gene Editing for the Correction of SCID-X1 Mutations

Author

Genovese, Pietro, primary, Schiroli, Giulia, additional, Conway, Anthony, additional, Ferrari, Samuele, additional, Guschin, Dmitry Y., additional, Di Tomaso, Tiziano, additional, Albano, Luisa, additional, Marin, Virna, additional, Giuliani, Erica, additional, Gregory, Philip D., additional, Holmes, Michael C., additional, Gentner, Bernhard, additional, Bovolenta, Chiara, additional, Cost, Gregory J., additional, Lombardo, Angelo, additional, and Naldini, Luigi, additional

Published
2016
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5. 703. Development of RD-MolPack-trLNGFR/dCK.DM.S74E Stable Packaging Cells

Author

Piovan, Claudia, primary, Giuliani, Erica, additional, Corna, Stefano, additional, Scavullo, Cinzia, additional, Bossi, Sergio, additional, Pema, Monika, additional, Medin, Jeffrey A., additional, McKillop, Monty, additional, Au, Bryan, additional, Bordignon, Claudio, additional, Rizzardi, Gian Paolo, additional, and Bovolenta, Chiara, additional

Published
2016
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7. 540. Vectofusin-1 Significantly Enhances Transduction of Ex-Vivo Activated T Cells With RD114-TR-Pseudotyped Lentiviral Vectors Derived from the RD3-MolPack Stable Packaging Cells

Author

Marin, Virna, primary, Corna, Stefano, additional, Giuliani, Erica, additional, Scavullo, Cinzia, additional, Bossi, Sergio, additional, Bordignon, Claudio, additional, Galy, Anne, additional, Paolo Rizzardi, Gian, additional, Fenard, David, additional, and Bovolenta, Chiara, additional

Published
2015
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8. Transcriptional Enhancers Induce Insertional Gene Deregulation Independently From the Vector Type and Design

Author

Maruggi, Giulietta, primary, Porcellini, Simona, additional, Facchini, Giulia, additional, Perna, Serena K, additional, Cattoglio, Claudia, additional, Sartori, Daniela, additional, Ambrosi, Alessandro, additional, Schambach, Axel, additional, Baum, Christopher, additional, Bonini, Chiara, additional, Bovolenta, Chiara, additional, Mavilio, Fulvio, additional, and Recchia, Alessandra, additional

Published
2009
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10. Lentiviral Vector Gene Therapy Protects XCGD Mice From Acute Staphylococcus aureusPneumonia and Inflammatory Response

Author

Farinelli, Giada, Jofra Hernandez, Raisa, Rossi, Alice, Ranucci, Serena, Sanvito, Francesca, Migliavacca, Maddalena, Brombin, Chiara, Pramov, Aleksandar, Di Serio, Clelia, Bovolenta, Chiara, Gentner, Bernhard, Bragonzi, Alessandra, and Aiuti, Alessandro

Abstract

Chronic granulomatous disease (CGD) is a primary immunodeficiency due to a deficiency in one of the subunits of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex. CGD patients are characterized by an increased susceptibility to bacterial and fungal infections, and to granuloma formation due to the excessive inflammatory responses. Several gene therapy approaches with lentiviral vectors have been proposed but there is a lack of in vivodata on the ability to control infections and inflammation. We set up a mouse model of acute infection that closely mimic the airway infection in CGD patients. It involved an intratracheal injection of a methicillin-sensitive reference strain of S. aureus. Gene therapy, with hematopoietic stem cells transduced with regulated lentiviral vectors, restored the functional activity of NADPH oxidase complex (with 20–98% of dihydrorhodamine positive granulocytes and monocytes) and saved mice from death caused by S. aureus, significantly reducing the bacterial load and lung damage, similarly to WT mice even at low vector copy number. When challenged, gene therapy-treated XCGD mice showed correction of proinflammatory cytokines and chemokine imbalance at levels that were comparable to WT. Examined together, our results support the clinical development of gene therapy protocols using lentiviral vectors for the protection against infections and inflammation.

Published
2016
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