1. EGFR-Targeted Nanobody Functionalized Polymeric Micelles Loaded with mTHPC for Selective Photodynamic Therapy
- Author
-
Liu, Yanna, Scrivano, Luca, Peterson, Julia Denise, Fens, Marcel H A M, Beltrán Hernández, Irati, Mesquita, Bárbara, Sastre Torano, Javier, Hennink, Wim E, van Nostrum, Cornelus F, Oliveira, Sabrina, Pharmaceutics, Chemical Biology and Drug Discovery, Celbiologie, Afd Pharmaceutics, Sub Membrane Biochemistry & Biophysics, Afd Chemical Biology and Drug Discovery, and Sub Cell Biology
- Subjects
Polymers ,medicine.medical_treatment ,Pharmaceutical Science ,Photodynamic therapy ,02 engineering and technology ,030226 pharmacology & pharmacy ,Micelle ,Polyethylene Glycols ,HeLa ,Mice ,chemistry.chemical_compound ,Drug Delivery Systems ,0302 clinical medicine ,Drug Discovery ,Photosensitizer ,Micelles ,Mice, Inbred BALB C ,Photosensitizing Agents ,biology ,Chemistry ,021001 nanoscience & nanotechnology ,ErbB Receptors ,Nanomedicine ,Mesoporphyrins ,photodynamic therapy ,Molecular Medicine ,Ethylene Glycols ,Female ,0210 nano-technology ,A431 cells ,Polyesters ,EGFR ,Mice, Nude ,Article ,03 medical and health sciences ,In vivo ,Cell Line, Tumor ,medicine ,Animals ,Humans ,targeting ,polymeric micelles ,selectivity ,Single-Domain Antibodies ,biology.organism_classification ,nanobody ,Photochemotherapy ,Cancer cell ,Chlorin ,Biophysics ,Nanoparticles ,HeLa Cells - Abstract
meta-Tetra(hydroxyphenyl)chlorin (mTHPC) is one of the most potent second-generation photosensitizers, clinically used for photodynamic therapy (PDT) of head and neck squamous cell carcinomas. However, improvements are still required concerning its present formulation (i.e., Foscan, a solution of mTHPC in ethanol/propylene glycol (40:60 w/w)), as mTHPC has the tendency to aggregate in aqueous media, e.g., biological fluids, and it has limited tumor specificity. In the present study, polymeric micelles with three different diameters (17, 24, and 45 nm) based on benzyl-poly(ε-caprolactone)-b-poly(ethylene glycol) (PCLn-PEG; n = 9, 15, or 23) were prepared with mTHPC loadings ranging from 0.5 to 10 wt % using a film-hydration method as advanced nanoformulations for this photosensitizer. To favor the uptake of the micelles by cancer cells that overexpress the epidermal growth factor receptor (EGFR), the micelles were decorated with an EGFR-targeted nanobody (named EGa1) through maleimide-thiol chemistry. The enhanced binding of the EGFR-targeted micelles at 4 °C to EGFR-overexpressing A431 cells, compared to low-EGFR-expressing HeLa cells, confirmed the specificity of the micelles. In addition, an enhanced uptake of mTHPC-loaded micelles by A431 cells was observed when these were decorated with the EGa1 nanobody, compared to nontargeted micelles. Both binding and uptake of targeted micelles were blocked by an excess of free EGa1 nanobody, demonstrating that these processes occur through EGFR. In line with this, mTHPC loaded in EGa1-conjugated PCL23-PEG (EGa1-P23) micelles demonstrated 4 times higher photocytotoxicity on A431 cells, compared to micelles lacking the nanobody. Importantly, EGa1-P23 micelles also showed selective PDT against A431 cells compared to the low-EGFR-expressing HeLa cells. Finally, an in vivo pharmacokinetic study shows that after intravenous injection, mTHPC incorporated in the P23 micelles displayed prolonged blood circulation kinetics, compared to free mTHPC, independently of the presence of EGa1. Thus, these results make these micelles a promising nanomedicine formulation for selective therapy.
- Published
- 2020
- Full Text
- View/download PDF