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Start Over Author "D Bisch" Journal molecular microbiology
2 results on '"D Bisch"'

1. Identification and analysis of genes from Streptomyces pristinaespiralis encoding enzymes involved in the biosynthesis of the 4-dimethylamino-L-phenylalanine precursor of pristinamycin I

Author

V, Blanc, P, Gil, N, Bamas-Jacques, S, Lorenzon, M, Zagorec, J, Schleuniger, D, Bisch, F, Blanche, L, Debussche, J, Crouzet, and D, Thibaut

Subjects
Molecular Structure, Sequence Homology, Amino Acid, Genes, Bacterial, Phenylalanine, Molecular Sequence Data, Sequence Analysis, DNA, Cloning, Molecular, Sequence Alignment, Virginiamycin, Streptomyces
Abstract

Four pap genes (papA, papB, papC, papM) were found by sequencing near to snbA, a Streptomyces pristinaespiralis gene which was previously shown to encode one of the pristinamycin I (PI) synthetases. Analysis of the homologies observed from the deduced amino acid sequences suggested that these four genes could be involved in the biosynthesis of the PI precursor 4-dimethylamino-L-phenylalanine (DMPAPA). This was first verified when disruption of papA in S. pristinaespiralis led to a PI- phenotype, which was reversed by the addition of DMPAPA into the culture medium. Further confirmation was obtained when papM was overexpressed in Escherichia coli and the corresponding protein purified to homogeneity. It catalysed the two successive N-methylation steps of 4-amino-L-phenylalanine leading to DMPAPA via 4-methylamino-L-phenylalanine. These results allowed us to assign a function to each of the four pap genes and to propose a biosynthetic pathway for DMPAPA.

Published
1997

2. Identification and analysis of genes from Streptomyces pristinaespiralis encoding enzymes involved in the biosynthesis of the 4-dimethylamino-L-phenylalanine precursor of pristinamycin I.

Author

Blanc V, Gil P, Bamas-Jacques N, Lorenzon S, Zagorec M, Schleuniger J, Bisch D, Blanche F, Debussche L, Crouzet J, and Thibaut D

Subjects
Cloning, Molecular, Genes, Bacterial physiology, Molecular Sequence Data, Molecular Structure, Phenylalanine biosynthesis, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Genes, Bacterial genetics, Phenylalanine analogs & derivatives, Streptomyces enzymology, Virginiamycin biosynthesis, Virginiamycin chemistry
Abstract

Four pap genes (papA, papB, papC, papM) were found by sequencing near to snbA, a Streptomyces pristinaespiralis gene which was previously shown to encode one of the pristinamycin I (PI) synthetases. Analysis of the homologies observed from the deduced amino acid sequences suggested that these four genes could be involved in the biosynthesis of the PI precursor 4-dimethylamino-L-phenylalanine (DMPAPA). This was first verified when disruption of papA in S. pristinaespiralis led to a PI- phenotype, which was reversed by the addition of DMPAPA into the culture medium. Further confirmation was obtained when papM was overexpressed in Escherichia coli and the corresponding protein purified to homogeneity. It catalysed the two successive N-methylation steps of 4-amino-L-phenylalanine leading to DMPAPA via 4-methylamino-L-phenylalanine. These results allowed us to assign a function to each of the four pap genes and to propose a biosynthetic pathway for DMPAPA.

Published
1997
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