1. Selection of membrane protein targets for crystallization using PFO-PAGE electrophoresis.
- Author
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Cleverley RM, Saleem M, Kean J, Ford RC, Derrick JP, and Prince SM
- Subjects
- ATP-Binding Cassette Transporters chemistry, ATP-Binding Cassette Transporters isolation & purification, Bacterial Proteins chemistry, Cation Transport Proteins chemistry, Cation Transport Proteins isolation & purification, Cell Membrane chemistry, Chromatography, Gel, Crystallization, Detergents metabolism, Membrane Transport Proteins isolation & purification, Protein Structure, Quaternary, Bacterial Proteins isolation & purification, Caprylates, Electrophoresis, Polyacrylamide Gel, Fluorocarbons, Membrane Transport Proteins chemistry
- Abstract
A method to rapidly assess the oligomeric composition of multimeric proteins is notably absent from reported schemes for high throughput production and crystallization of membrane proteins. In this report we have investigated the suitability of PFO-PAGE electrophoresis for this purpose and present examples where it proves highly informative in selecting conditions favouring the functional oligomeric state of the target protein. Features such as the ability to analyze several samples in parallel, including crude membrane extracts, suggest it will be highly adaptable to high throughput analysis of membrane proteins.
- Published
- 2008
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