1. Identification of differentially expressed microRNAs in acute Kawasaki disease
- Author
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Yue yue Ding, Ye Chen, Ming Guo Xu, Lei Cao, Yan Ren, Qiu qin Xu, Ling Sun, and Haitao Lv
- Subjects
0301 basic medicine ,Male ,Cancer Research ,Microarray ,Biology ,Mucocutaneous Lymph Node Syndrome ,Biochemistry ,Pathogenesis ,03 medical and health sciences ,microRNA ,Genetics ,Humans ,Gene Regulatory Networks ,RNA, Messenger ,KEGG ,Molecular Biology ,Gene ,Oncogene ,Kawasaki disease ,target gene ,Microarray analysis techniques ,Gene Expression Profiling ,Computational Biology ,Infant ,Articles ,Molecular biology ,microRNA-223-3p ,microRNAs ,body regions ,030104 developmental biology ,Gene Ontology ,Oncology ,Gene Expression Regulation ,Case-Control Studies ,Child, Preschool ,Gene Ontology Term Enrichment ,embryonic structures ,Acute Disease ,Cancer research ,Molecular Medicine ,Female ,Transcriptome ,microarray ,Signal Transduction - Abstract
The present study used microarray analysis to screen the plasma expression of microRNAs (miRNAs) in patients with acute Kawasaki disease (KD) and aimed to explore the pathogenesis of KD. Plasma was collected from children with acute KD (n=6) and from healthy control children (n=6). Total RNA was extracted and differential miRNA expression between the two groups was determined. Differentially expressed miRNAs were validated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in an independent cohort (n=8). Target genes of the differentially expressed miRNAs were predicted and analyzed for gene ontology term enrichment and Kyoto Encyclopedia of Genes and Genomes pathways. miRNA microarray analysis revealed that seven miRNAs (miRs) were significantly upregulated (hsa-let-7b-5p, hsa-miR-223-3p, hsa-miR-4485, hsa-miR-4644, hsa-miR-4800-5p, hsa-miR-6510-5p and hsa-miR-765) and three were significantly downregulated (hsa-miR-33b-3p, hsa-miR-4443 and hsa-miR-4515) in acute KD compared with the healthy controls. hsa-miR-223-3p expression levels detected by RT-qPCR were consistent with the microarray results. A total of 62 target genes of hsa-miR-223-3p were predicted. In total, 10 differentially expressed miRNAs were identified in acute KD, of which hsa-miR-223-3p was verified by RT-qPCR.
- Published
- 2017