1. FHL1 inhibits the growth of tongue squamous cell carcinoma cells via G1/S cell cycle arrest
- Author
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Peiyun Du, Panfeng Lian, Fengjun Xiao, Xin Huang, Zhenyang Gao, Qinong Ye, Hongyuan Wang, Enqun Wang, Long Cheng, Wei Ren, Xin Guan, Lihua Ding, Xiaolin Bi, and Lisheng Wang
- Subjects
Cancer Research ,Small interfering RNA ,Cyclin E ,Cyclin D ,Cell ,Transplantation, Heterologous ,Mice, Nude ,Muscle Proteins ,Biology ,Real-Time Polymerase Chain Reaction ,Biochemistry ,Mice ,Cell Movement ,Cell Line, Tumor ,Genetics ,medicine ,Animals ,Humans ,RNA, Small Interfering ,Promoter Regions, Genetic ,Molecular Biology ,Gene knockdown ,Mice, Inbred BALB C ,Oncogene ,Cell growth ,Intracellular Signaling Peptides and Proteins ,Cell cycle ,DNA Methylation ,LIM Domain Proteins ,Molecular biology ,G1 Phase Cell Cycle Checkpoints ,Tongue Neoplasms ,medicine.anatomical_structure ,Oncology ,S Phase Cell Cycle Checkpoints ,biology.protein ,Cancer research ,Carcinoma, Squamous Cell ,Molecular Medicine ,RNA Interference - Abstract
Four and a half LIM protein 1 (FHL1) has been characterized as a tumor suppressor in various types of tumor. However, the biological function and underlying mechanism of FHL1 in tongue squamous cell carcinoma (TSCC) remain to be elucidated. The present study demonstrated that FHL1 inhibits anchorage‑dependent and ‑independent growth of TSCC cells in vitro and tumor growth in nude mice, as determined by cell proliferation and soft agar assays. Knockdown of FHL1 with FHL1 small interfering RNA (siRNA) promoted tumor growth in nude mice. Mechanistically, flow cytometric analysis showed that knockdown of FHL1 promoted G1/S cell cycle progression. Furthermore, expression of cell cycle‑associated regulators, cyclin D and cyclin E, were detected by western blotting and reverse transcription‑quantitative polymerase chain reaction. Cyclin D and cyclin E were markedly elevated at both the protein and mRNA level in the FHL1 siRNA‑transfected cells. These results suggested that FHL1 has a tumor suppressive role in TSCC and that FHL1 may be a useful target for TSCC gene therapy.
- Published
- 2014