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Your search keyword '"Chin‐San Liu"' showing total 4 results
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4 results on '"Chin‐San Liu"'

1. Sinulariolide suppresses LPS‑induced phenotypic and functional maturation of dendritic cells

Author

Wei Yuan Huang, Ting Wen Chung, Yi Rong Li, Shih‑Chao Lin, Chi Chien Lin, Jui-Hsin Su, Hong-Lin Chan, Ching‑Hsiung Lin, Sheng Hao Lin, and Chin‑San Liu

Subjects
0301 basic medicine, CD4-Positive T-Lymphocytes, Lipopolysaccharides, Cancer Research, Lipopolysaccharide, Lymphocyte Activation, 01 natural sciences, Biochemistry, Immune tolerance, chemistry.chemical_compound, Mice, Cells, Cultured, biology, NF-kappa B, hemic and immune systems, Interleukin-12, Cell biology, medicine.anatomical_structure, Phenotype, Oncology, B7-1 Antigen, Molecular Medicine, Female, Diterpenes, Cell Survival, T cell, chemical and pharmacologic phenomena, Bone Marrow Cells, Enzyme-Linked Immunosorbent Assay, Nitric Oxide, 03 medical and health sciences, Genetics, medicine, Animals, CD40 Antigens, Molecular Biology, Cell Proliferation, CD86, CD40, 010405 organic chemistry, Interleukin-6, Dendritic cell, Dendritic Cells, 0104 chemical sciences, Mice, Inbred C57BL, 030104 developmental biology, chemistry, biology.protein, Cancer research, Cytokine secretion, B7-2 Antigen, CD80
Abstract

The dendritic cell (DC) maturation process is essential for the development of T cell responses and immune tolerance. Accordingly, DCs are considered a major target in the development of immunomodulating agents. In the present study, the effect of sinulariolide, an active compound isolated from the cultured soft coral Sinularia flexibilis, on lipopolysaccharide (LPS)‑induced murine bone marrow‑derived DCs was evaluated. The different phenotypes, cytokine secretion and the mix‑lymphocyte reaction of DCs were detected using flow cytometry and ELISA. The experimental results revealed that the phenotypic and functional maturation of DCs stimulated by LPS were markedly reduced by sinulariolide in a concentration‑dependent manner, including the expression of co‑stimulatory molecules (CD40, CD80 and CD86). In addition, sinulariolide reduced the release of tumor necrosis factor‑α, interleukin (IL)‑6, IL‑12 and nitric oxide from the LPS‑activated DCs, decreased their abilities to stimulate allogeneic T cell proliferation, and inhibited LPS‑induced nuclear factor‑κB pathways. These findings offer novel insight into the immunopharmacological function of sinulariolide and its effects on DCs.

Published
2016

2. H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes

Author

Chin‑San Liu, Shih‑Li Su, Wei‑Wen Lin, and Ya‑Hui Chen

Subjects
0301 basic medicine, Cancer Research, Glutamate-Cysteine Ligase, Caveolin 1, Oxidative phosphorylation, Mitochondrion, medicine.disease_cause, Biochemistry, Mitochondrial Membrane Transport Proteins, Oxidative Phosphorylation, Podocyte, Cell Line, GTP Phosphohydrolases, Superoxide dismutase, 03 medical and health sciences, Mice, Bcl-2-associated X protein, Mitochondrial Precursor Protein Import Complex Proteins, Genetics, medicine, Animals, Amino Acid Sequence, Molecular Biology, bcl-2-Associated X Protein, chemistry.chemical_classification, Reactive oxygen species, biology, Podocytes, Superoxide Dismutase, Membrane Proteins, Hydrogen Peroxide, medicine.disease, Catalase, Cell biology, Mitochondria, 030104 developmental biology, medicine.anatomical_structure, Oncology, chemistry, Gene Expression Regulation, Proto-Oncogene Proteins c-bcl-2, cardiovascular system, biology.protein, Molecular Medicine, Optic Atrophy 1, Peptides, Reactive Oxygen Species, Oxidative stress, Signal Transduction
Abstract

Increased intercellular reactive oxygen species (ROS) levels are the major cause of podocyte injury with proteinuria. Caveolin‑1 (CAV‑1) is an essential protein component of caveolae. CAV‑1 participates in signal transduction and endocytic trafficking. Recent research has indicated that CAV‑1 regulates oxidative stress‑induced pathways. The present study used hydrogen peroxide (H2O2) at nontoxic concentrations to elevate the level of ROS in E11 podocytes. Treatment with 500 and 1,000 µM H2O2 for 1 h significantly reduced CAV‑1 expression levels. Simultaneously, the treatment significantly reduced the expression of the antioxidant enzymes glutamine‑cysteine ligase catalytic subunit, superoxide dismutase 2 and catalase. To determine the role of CAV‑1 in mediating oxidative stress, E11 podocytes were administered antenapedia‑CAV‑1 (AP‑CAV‑1) peptide for 48 h. The AP‑CAV‑1 treatment enhanced CAV‑1 expression and inhibited cyclophilin A expression, thus reducing ROS‑induced inflammation. Moreover, CAV‑1 protected against H2O2‑induced oxidative stress responses by enhancing the expression of antioxidant enzymes. Furthermore, CAV‑1 attenuated H2O2‑induced changes oxidative phosphorylation, and the expression of optic atrophy 1 and translocase of the inner membrane 23, as well as preserving mitochondrial function. CAV‑1 treatment significantly suppressed apoptosis, as indicated by a higher B‑cell lymphoma 2/BCL2‑associated X protein ratio. Therefore, enhancing the expression of CAV‑1 may be an important therapeutic consideration in treating podocyte injury.

Published
2016

3. H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes.

Author

YA‑HUI CHEN, CHIN‑SAN LIU, SHIH‑LI SU, and WEI‑WEN LIN

Subjects
*PHYSIOLOGICAL effects of hydrogen peroxide, *CAVEOLINS, *MITOCHONDRIAL physiology, *APOPTOSIS, *ACTIVE oxygen in the body, *CELLULAR signal transduction
Abstract

Increased intercellular reactive oxygen species (ROS) levels are the major cause of podocyte injury with proteinuria. Caveolin‑1 (CAV‑1) is an essential protein component of caveolae. CAV‑1 participates in signal transduction and endocytic trafficking. Recent research has indicated that CAV‑1 regulates oxidative stress‑induced pathways. The present study used hydrogen peroxide (H2O2) at nontoxic concentrations to elevate the level of ROS in E11 podocytes. Treatment with 500 and 1,000 μM H2O2 for 1 h significantly reduced CAV‑1 expression levels. Simultaneously, the treatment significantly reduced the expression of the antioxidant enzymes glutamine‑cysteine ligase catalytic subunit, superoxide dismutase 2 and catalase. To determine the role of CAV‑1 in mediating oxidative stress, E11 podocytes were administered antenapedia‑CAV‑1 (AP‑CAV‑1) peptide for 48 h. The AP‑CAV‑1 treatment enhanced CAV‑1 expression and inhibited cyclophilin A expression, thus reducing ROS‑induced inflammation. Moreover, CAV‑1 protected against H2O2‑induced oxidative stress responses by enhancing the expression of antioxidant enzymes. Furthermore, CAV‑1 attenuated H2O2‑induced changes oxidative phosphorylation, and the expression of optic atrophy 1 and translocase of the inner membrane 23, as well as preserving mitochondrial function. CAV‑1 treatment significantly suppressed apoptosis, as indicated by a higher B‑cell lymphoma 2/BCL2‑associated X protein ratio. Therefore, enhancing the expression of CAV‑1 may be an important therapeutic consideration in treating podocyte injury. [ABSTRACT FROM AUTHOR]

Published
2017
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4. Sinulariolide suppresses LPS‑induced phenotypic and functional maturation of dendritic cells.

Author

TING‑WEN CHUNG, HONG‑LIN CHAN, SHENG‑HAO LIN, CHING‑HSIUNG LIN, YI‑RONG LI, CHIN‑SAN LIU, WEI YUAN HUANG, CHI‑CHIEN LIN, JUI‑HSIN SU, and SHIH‑CHAO LIN

Subjects
DENDRITIC cells, BIOACTIVE compounds, ALCYONACEA, LIPOPOLYSACCHARIDES, IMMUNOREGULATION, PHENOTYPES, IMMUNOPHARMACOLOGY, PHYSIOLOGY
Abstract

The dendritic cell (DC) maturation process is essential for the development of T cell responses and immune tolerance. Accordingly, DCs are considered a major target in the development of immunomodulating agents. In the present study, the effect of sinulariolide, an active compound isolated from the cultured soft coral Sinularia flexibilis, on lipopolysaccharide (LPS)‑induced murine bone marrow‑derived DCs was evaluated. The different phenotypes, cytokine secretion and the mix‑lymphocyte reaction of DCs were detected using flow cytometry and ELISA. The experimental results revealed that the phenotypic and functional maturation of DCs stimulated by LPS were markedly reduced by sinulariolide in a concentration‑dependent manner, including the expression of co‑stimulatory molecules (CD40, CD80 and CD86). In addition, sinulariolide reduced the release of tumor necrosis factor‑α, interleukin (IL)‑6, IL‑12 and nitric oxide from the LPS‑activated DCs, decreased their abilities to stimulate allogeneic T cell proliferation, and inhibited LPS‑induced nuclear factor‑κB pathways. These findings offer novel insight into the immunopharmacological function of sinulariolide and its effects on DCs. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

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