1. Mechanism of interferon-γ down-regulation of the interleukin 4-induced CD23/FcϵRII expression in human B cells: Post-transcriptional modulation by interferon-γ
- Author
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Suk Ran Yoon, Kwang Ho Pyun, and Choong Eun Lee
- Subjects
B-Lymphocytes ,Messenger RNA ,Receptors, IgE ,medicine.medical_treatment ,Immunology ,CD23 ,Down-Regulation ,Biology ,Molecular biology ,Interferon-gamma ,Cytokine ,Gene Expression Regulation ,Gene expression ,medicine ,Protein biosynthesis ,Humans ,Interleukin 19 ,Interferon gamma ,Interleukin-4 ,RNA, Messenger ,RNA Processing, Post-Transcriptional ,Molecular Biology ,Interleukin 4 ,medicine.drug - Abstract
It has been reported that the interleukin 4 (IL-4) specific induction of cell surface CD23 (Fc epsilon RII) is down-regulated by interferon-gamma (IFN-gamma) in monocytes and B cells. However, the molecular level at which the inhibition occurs seems to vary depending on the cell types. In normal human B cells, IFN-gamma inhibits the IL-4 induced de novo synthesis of CD23 at the level of gene expression. Analysis of inhibition kinetics suggested a rapid signal transmission by IFN-gamma. Yet the inhibitory action of IFN-gamma on CD23 mRNA accumulation appeared as a secondary response requiring a new protein synthesis. Through nuclear run-on transcription and mRNA stability studies, we further demonstrate that the IL-4 induced CD23 gene expression is down-regulated by IFN-gamma mainly at post-transcriptional levels by decreasing mRNA stability.
- Published
- 1993
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