1. Evaluation of microRNA-10b prognostic significance in a prospective cohort of breast cancer patients
- Author
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Vito Michele Fazio, Massimiliano Copetti, Paola Parrella, Andrea Onetti-Muda, Teresa Balsamo, Evaristo Maiello, Maria Luana Poeta, Fabio Pellegrini, Michelina Coco, Andrea Fontana, Marina Castelvetere, Raffaela Barbano, Giuseppe Perrone, Barbara Pasculli, Vanna Maria Valori, Roberto Murgo, Daniela Righi, and Maria Morritti
- Subjects
Oncology ,Adult ,medicine.medical_specialty ,Cancer Research ,Breast Neoplasms ,RNA integrity number ,Metastasis ,Breast cancer ,Predictive Value of Tests ,Internal medicine ,medicine ,Biomarkers, Tumor ,Humans ,Prospective Studies ,Prospective cohort study ,Survival analysis ,Aged ,Neoplasm Staging ,Proportional Hazards Models ,Homeodomain Proteins ,microRNA ,Proportional hazards model ,business.industry ,Research ,RT-qPCR ,Middle Aged ,medicine.disease ,Prognosis ,Survival Analysis ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Predictive value of tests ,Lymphatic Metastasis ,Nottingham Prognostic Index ,Molecular Medicine ,Female ,Neoplasm Grading ,business ,Signal Transduction ,Transcription Factors - Abstract
Background MicroRNA-10b (miR-10b) has a prominent role in regulating tumor invasion and metastasis by targeting the HOXD10 transcriptional repressor and has been found up-regulated in several tumor types. Methods We evaluated the expression of miR-10b in paired tumor and normal specimens obtained from a prospective cohort of breast cancer patients with at least 36 months follow-up enrolled according to the REMARK guidelines (n = 150). RNA quality was measured and only samples with RNA Integrity Number (RIN) ≥7.0 were analyzed. Results The relative expression of miR-10b in tumor as compared to its normal counterpart (RER) was determined by RT-qPCR. miR-10b RERs were higher in the subgroup of patients with synchronous metastases (n = 11, Median 0.25; IQR 0.11-1.02) as compared with patients without metastases (n = 90, Median 0.09; IQR 0.04-0.29) (p = 0.028). In the subgroup of patients without synchronous metastases (n = 90), higher miR-10b RERs were associated with increased risk of disease progression and death in both univariable (HR 1.16, p = 0.021 and HR 1.20, p = 0.015 respectively for 0.10 unitary increase of miR-10b RERs levels) and multivariable (HR1.30, p
- Published
- 2014