5 results on '"Maffia, M"'
Search Results
2. Comparative proteomic profiling of Hodgkin lymphoma cell lines
- Author
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Claudio Agostinelli, S. De Matteis, Marco Marchisio, Michele Maffia, Pasquale Simeone, Sebastiano Miscia, Silvia Carloni, Paola Lanuti, Daniele Vergara, Roberta Napolitano, Antonio Rizzello, Vergara, D., Simeone, P., De Matteis, S., Carloni, S., Lanuti, P., Marchisio, M., Miscia, S., Rizzello, A., Napolitano, R., Agostinelli, C., Maffia, M., Vergara, Daniele, Simeone, P, De Matteis, S, Carloni, S, Lanuti, P, Marchisio, M, Miscia, S, Rizzello, Antonia, Napolitano, R, Agostinelli, C, and Maffia, Michele
- Subjects
Proteomics ,0301 basic medicine ,Proteome ,macromolecular substances ,Biology ,Models, Biological ,Flow cytometry ,Pathogenesis ,03 medical and health sciences ,Cell Line, Tumor ,Protein Interaction Mapping ,medicine ,Humans ,Protein Interaction Maps ,Molecular Biology ,medicine.diagnostic_test ,Proteomic Profiling ,Proteomic ,Cell migration ,Flow Cytometry ,Hodgkin Disease ,Molecular biology ,Gene Expression Regulation, Neoplastic ,Blot ,030104 developmental biology ,Cell culture ,Protein Interaction Map ,Human ,Biotechnology - Abstract
Classical Hodgkin lymphoma (cHL) is a malignancy with complex pathogenesis. The hallmark of the disease is the presence of large mononucleated Hodgkin and bi- or multinucleated Reed/Sternberg (H/RS) cells. The origin of HRS cells in cHL is controversial as these cells show the coexpression of markers of several lineages. Using a proteomic approach, we compared the protein expression profile of cHL models of T- and B-cell derivation to find proteins differentially expressed in these cell lines. A total of 67 proteins were found differentially expressed between the two cell lines including metabolic proteins and proteins involved in the regulation of the cytoskeleton and/or cell migration, which were further validated by western blotting. Additionally, the expression of selected B- and T-cell antigens was also assessed by flow cytometry to reveal significant differences in the expression of different surface markers. Bioinformatics analysis was then applied to our dataset to find enriched pathways and networks, and to identify possible key regulators. In the present study, a proteomic approach was used to compare the protein expression profiles of two cHL cell lines. The identified proteins and/or networks, many of which not previously related to cHL, may be important to better define the pathogenesis of the disease, to identify novel diagnostic markers, and to design new therapeutic strategies.
- Published
- 2016
3. Anticancer effects of novel resveratrol analogues on human ovarian cancer cells.
- Author
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Vergara D, De Domenico S, Tinelli A, Stanca E, Del Mercato LL, Giudetti AM, Simeone P, Guazzelli N, Lessi M, Manzini C, Santino A, Bellina F, and Maffia M
- Subjects
- Antineoplastic Agents, Phytogenic chemistry, Cell Line, Tumor, Cell Movement drug effects, Epidermal Growth Factor pharmacology, Epithelial-Mesenchymal Transition drug effects, Female, Humans, Interleukin-6 pharmacology, Resveratrol, Signal Transduction drug effects, Stilbenes chemistry, Antineoplastic Agents, Phytogenic pharmacology, Ovarian Neoplasms metabolism, Stilbenes pharmacology
- Abstract
Resveratrol, a naturally occurring phytoalexin, has long been known to play an important regulatory role in key functions in cell physiology. This multifunctional role of resveratrol is explained by its ability to interact with several targets of various cell pathways. In the recent past, synthetic chemical modifications have been made in an attempt to enhance the biological effects of resveratrol, including its anti-cancer properties. In this study, we investigated the molecular mechanisms of action of novel trans-restricted analogues of resveratrol in which the C-C double bond of the natural derivative has been replaced by diaryl-substituted imidazole analogues. In ovarian cancer models, the results of in vitro screening revealed that the resveratrol analogues exhibited enhanced anti-proliferative properties compared with resveratrol. We found that the resveratrol analogues also significantly inhibited Akt and MAPK signalling and reduced the migration of IL-6 and EGF-treated cells. Finally, in ascite-derived cancer cells, we demonstrated that the resveratrol analogues reduced the expression of epithelial mesenchymal transition (EMT) markers. Collectively, these findings indicate the enhanced anti-cancer properties of the resveratrol analogues.
- Published
- 2017
- Full Text
- View/download PDF
4. Comparative proteome profiling of breast tumor cell lines by gel electrophoresis and mass spectrometry reveals an epithelial mesenchymal transition associated protein signature.
- Author
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Vergara D, Simeone P, del Boccio P, Toto C, Pieragostino D, Tinelli A, Acierno R, Alberti S, Salzet M, Giannelli G, Sacchetta P, and Maffia M
- Subjects
- Antineoplastic Agents pharmacology, Breast Neoplasms genetics, Cell Adhesion, Cell Line, Tumor, Cell Movement, Cell Proliferation, Cell Survival drug effects, Female, Gene Expression, Gene Expression Profiling, Humans, Mesoderm metabolism, Neoplasm Invasiveness, Neoplasm Metastasis, Niacinamide analogs & derivatives, Niacinamide pharmacology, Phenylurea Compounds pharmacology, Piperidines pharmacology, Protein Interaction Maps, Protein Kinases metabolism, Proteome genetics, Proteome metabolism, Proteomics, Quinazolines pharmacology, Sorafenib, Breast Neoplasms metabolism, Epithelial Cells metabolism, Epithelial-Mesenchymal Transition genetics, Mesoderm cytology
- Abstract
The epithelial to mesenchymal transition (EMT) is a cellular program associated with the organ morphogenesis but also with the disease progression. EMT in the cancer field fuels neoplastic progression promoting the resistance to cell death, the resistance to chemotherapy and the acquisition of stem cell properties. Considering the crucial role of EMT in breast cancer metastasis, a better understanding of this process may provide new therapeutic options. Here, by using a proteomic approach we identified a set of proteins differentially expressed between an epithelial and a mesenchymal breast cancer cell line. The protein-protein network of these identified proteins was determined by an in silico analysis highlighting, in the EMT program, the role of proteins involved in cell adhesion, migration, and invasion, together with protein kinases involved in proliferation and survival, with many of these emerging as possible targets of novel biological agents. Finally, the pharmacological inhibition of some of these kinases was able to reverse the mesenchymal phenotype to an epithelial phenotype.
- Published
- 2013
- Full Text
- View/download PDF
5. Resveratrol downregulates Akt/GSK and ERK signalling pathways in OVCAR-3 ovarian cancer cells.
- Author
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Vergara D, Simeone P, Toraldo D, Del Boccio P, Vergaro V, Leporatti S, Pieragostino D, Tinelli A, De Domenico S, Alberti S, Urbani A, Salzet M, Santino A, and Maffia M
- Subjects
- Cell Line, Tumor, Cell Proliferation drug effects, Cyclin D1 genetics, Cyclin D1 metabolism, Down-Regulation, Extracellular Signal-Regulated MAP Kinases genetics, Female, Glycogen Synthase Kinase 3 genetics, Glycogen Synthase Kinase 3 beta, Humans, Microscopy, Confocal, Ovarian Neoplasms metabolism, Phosphatidylinositol 3-Kinases genetics, Phosphatidylinositol 3-Kinases metabolism, Phosphorylation drug effects, Proteomics methods, Proto-Oncogene Proteins c-akt genetics, Resveratrol, Antineoplastic Agents pharmacology, Extracellular Signal-Regulated MAP Kinases metabolism, Glycogen Synthase Kinase 3 metabolism, Proto-Oncogene Proteins c-akt metabolism, Signal Transduction drug effects, Stilbenes pharmacology
- Abstract
Phytochemicals constitute a heterogeneous group of substances with an evident role in human health. Their properties on cancer initiation, promotion and progression are well documented. Particular attention is now devoted to better understand the molecular basis of their anticancer action. In the present work, we studied the effect of resveratrol on the ovarian cancer cell line OVCAR-3 by a proteomic approach. Our findings demonstrate that resveratrol down-regulates the protein cyclin D1 and, in a concentration dependent manner, the phosphorylation levels of protein kinase B (Akt) and glycogen synthase kinase-3β (GSK-3β). The dephosphorylation of these kinases could be responsible for the decreased cyclin D1 levels observed after treatment. We also showed that resveratrol reduces phosphorylation levels of the extracellular signal-regulated kinase (ERK) 1/2. Chemical inhibitors of phosphatidylinositol 3-kinase (PI3K) and ERK both increased the in vitro therapeutic efficacy of resveratrol. Moreover, resveratrol had an inhibitory effect on the AKT phosphorylation in cultured cells derived from the ascites of ovarian cancer patients and in a panel of human cancer cell lines. Thus, resveratrol shows antitumor activity in human ovarian cancer cell lines targeting signalling pathway involved in cell proliferation and drug-resistance.
- Published
- 2012
- Full Text
- View/download PDF
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