1. Overexpression of MuHSP70 gene from Macrotyloma uniflorum confers multiple abiotic stress tolerance in transgenic Arabidopsis thaliana
- Author
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Shikha Masand and Sudesh Kumar Yadav
- Subjects
Chlorophyll ,Models, Molecular ,0106 biological sciences ,0301 basic medicine ,Salinity ,Hot Temperature ,Recombinant Fusion Proteins ,Transgene ,Molecular Sequence Data ,Arabidopsis ,Sodium Chloride ,Plant Roots ,01 natural sciences ,Macrotyloma ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Stress, Physiological ,Heat shock protein ,Botany ,Genetics ,Arabidopsis thaliana ,HSP70 Heat-Shock Proteins ,Amino Acid Sequence ,Cloning, Molecular ,Molecular Biology ,Gene ,Plant Proteins ,biology ,Abiotic stress ,Water ,Fabaceae ,Hydrogen Peroxide ,General Medicine ,Plants, Genetically Modified ,biology.organism_classification ,Adaptation, Physiological ,Droughts ,Hsp70 ,Cell biology ,Cold Temperature ,Oxidative Stress ,030104 developmental biology ,Sequence Alignment ,Plant Shoots ,010606 plant biology & botany - Abstract
A 70-KD heat shock protein (HSP70) is one of the most conserved chaperones. It is involved in de novo protein folding and prevents the aggregation of unfolded proteins under lethal environmental factors. The purpose of this study is to characterise a MuHSP70 from horsegram (Macrotyloma uniflorum) and elucidating its role in stress tolerance of plants. A MuHSP70 was cloned and characterised from a natural drought stress tolerant HPK4 variety of horsegram (M. uniflorum). For functional characterization, MuHSP70 was overexpressed in transgenic Arabidopsis. Overexpression of MuHSP70 was found to provide tolerance to the transgenic Arabidopsis against various stresses such as heat, cold, drought, salinity and oxidative stress. MuHSP70 transgenics were observed to maintain the shoot biomass, root length, relative water content, and chlorophyll content during exposure to multi-stresses relative to non-transgenic control. Transgenic lines have further shown the reduced levels of MDA, H2O2, and proteolytic activity. Together, these findings suggest that overexpression of MuHSP70 plays an important role in improving abiotic stress tolerance and could be a crucial candidate gene for exploration in crop improvement program. more...
- Published
- 2015
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