1. Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139
- Author
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Makoto Ohnishi, Eiji Arakawa, Dong Tu Nguyen, Masatomo Morita, Hidemasa Izumiya, Hoai Thu Nguyen, and Tuan Cuong Ngo
- Subjects
Loop-mediated isothermal amplification ,medicine.disease_cause ,Rapid detection ,Vibrio cholerae O139 ,Microbiology ,03 medical and health sciences ,Bacterial Proteins ,Cholera ,Limit of Detection ,medicine ,Humans ,Molecular Biology ,Gene ,030304 developmental biology ,0303 health sciences ,biology ,030306 microbiology ,Vibrio cholerae O1 ,Cell Biology ,medicine.disease ,biology.organism_classification ,Molecular Diagnostic Techniques ,Vibrio cholerae ,Nucleic Acid Amplification Techniques ,Bacteria ,Environmental Monitoring - Abstract
A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5–67 copies per reaction in 1 h. The assay will aid rapid detection of the cholera bacterium.
- Published
- 2019