1. Down regulation of melanin concentrating hormone in virally induced obesity
- Author
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Marie-Françoise Belin, Olivier Verlaeten, Arlette Bernard, Seng-Thuon Khuth, Bernadette Griffond, Dominique Fellmann, Pascale Giraudon, Hideo Akaoka, Centre de recherche en neurosciences de Lyon (CRNL), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Melanin-concentrating hormone ,[SDV]Life Sciences [q-bio] ,MESH: Base Sequence ,Biochemistry ,MESH: Down-Regulation ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Endocrinology ,MESH: Melanins ,MESH: Reverse Transcriptase Polymerase Chain Reaction ,MESH: Obesity ,MESH: Animals ,Distemper Virus, Canine ,0303 health sciences ,Differential display ,Hypothalamic Hormones ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,3. Good health ,MESH: Pituitary Hormones ,Hypothalamus ,Acute Disease ,MESH: Acute Disease ,medicine.medical_specialty ,MESH: Distemper ,Molecular Sequence Data ,Down-Regulation ,Virus ,Measles virus ,03 medical and health sciences ,Downregulation and upregulation ,Internal medicine ,medicine ,MESH: Distemper Virus, Canine ,Animals ,Obesity ,RNA, Messenger ,Distemper ,Molecular Biology ,MESH: Mice ,030304 developmental biology ,MESH: RNA, Messenger ,Melanins ,MESH: Molecular Sequence Data ,Base Sequence ,Canine distemper ,medicine.disease ,biology.organism_classification ,MESH: Hypothalamus ,MESH: Hypothalamic Hormones ,Pituitary Hormones ,chemistry ,Immunology ,030217 neurology & neurosurgery ,Homeostasis - Abstract
International audience; Obesity is a complex disease involving genetic components and environmental factors and probably associated with the dysregulation of central homeostasis normally maintained by the hypothalamic neuroendocrine/neurotransmitter network. We previously reported that canine distemper virus (CDV), which is closely related to human measles virus, can target hypothalamic nuclei, and lead to obesity syndrome in the late stages of infection. Here, using differential display PCR, we demonstrate specific down-regulation of melanin-concentrating hormone precursor mRNA (ppMCH) in infected-obese mice. Although ppMCH was down-regulated in all infected mice during the acute stage of infection, this was only seen during the late stage of infection in infected-obese mice. In addition, ppMCH mRNA and protein expression in the lateral hypothalamus was decreased in the absence of neuronal death. These results show the importance of ppMCH in the establishment and maintenance of obesity and the involvement of a virus as an environmental factor.
- Published
- 2001