1. Weak but Uniform Enrichment of the Histone Variant macroH2A1 along the Inactive X Chromosome.
- Author
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Mietton, Flore, Sengupta, Aditya K., Molla, Annie, Picchi, Gisele, Barral, Sophie, Heliot, Laurent, Grange, Thierry, Wutz, Anton, and Dimitrov, Stefan
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HISTONES , *CHROMOSOMES , *GREEN fluorescent protein , *MOLECULAR biology , *BIOCHEMISTRY education , *CHROMATIN , *PROTEIN microarrays - Abstract
We studied the enrichment and distribution of the histone variant mH2A1 in the condensed inactive X (Xi) chromosome. By using highly specific antibodies against mH2A1 and stable HEK 293 cell lines expressing either green fluorescent protein (GFP)-mH2A1 or GFP-H2A, we found that the Xi chromosome contains ∼1.5-fold more mH2A1 than the autosomes. To determine the in vivo distribution of mH2A1 along the X chromosome, we used a native chromatin immunoprecipitation-on-chip technique. DNA isolated from mH2A1-immunoprecipitated nucleosomes from either male or female mouse liver were hybridized to tiling microarrays covering 5 kb around most promoters or the entire X chromosome. The data show that mH2A1 is uniformly distributed across the entire Xi chromosome. Interestingly, a stronger mH2A1 enrichment along the pseudoautosomal X chromosome region was observed in both sexes. Our results indicate a potential role for macroH2A in large-scale chromosome structure and genome stability. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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