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Cytochrome B (Cyt B) gene and control region of mitochondrial DNA are considered important for evaluating phylogenetic association of a species. In this study, we sequenced Cyt B and control region of Schizothorax plagiostomus and constructed phylogenetic association tree of S. plagiostomus with 23 schizothoracine fishes. We found S. plagiostomus to be closely associated with S. labiatus, S. richardsonii, S. progastus, and S. esocinus, with high-bootstraps values. Several conserved sequence blocks were identified within D-loop sequences. These are highly conserved within genus Schizothorax compared to other. This study reports the phylogenetic position of the S. plagiostomus among schizothoracines fishes and organization of D-loop region in S. plagiostomus from Pakistan.

The complete mitogenome of Triplophysa cuneicephala is 16,571 bp in length with the A + T contents of 56.12%, containing 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and a putative control region. All PCGs employed ATG as the start codon, except for COI which utilized GTG as start codon. Four stop codons were observed in the PCGs, including TAA, TAG, TA and T. Phylogenetic analysis based on concatenated PCG sequences revealed that T. cuneicephala is closely related to T. robusta and T. minxianensis. This study would provide useful genetic information for future studies on phylogeny, evolutionary, and taxonomic classification in Triplophysa species.

The complete mitochondrial genome of Triplophysa sellaefer is a circular molecule of 16,571 bp in length with the A + T contents of 56.84%, containing 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and a putative control region. Six gene overlaps and 10 intergenic spacers were observed, with the total length of 26 bp and 34 bp, respectively. The phylogenetic analysis based on the concatenated PCG sequences revealed that T. sellaefer is closely related to T. alticeps. This study would provide useful information on phylogenetic and evolutionary studies in Triplophysa.