1. Trace determination of metal ion inhibitors of the glucose-glucose oxidase system
- Author
-
E. Clifford Toren and F. Joseph Burger
- Subjects
Silver ,Inorganic chemistry ,chemistry.chemical_element ,Maximum error ,Horseradish peroxidase ,Analytical Chemistry ,Metal ,Glucose Oxidase ,chemistry.chemical_compound ,Methods ,Glucose oxidase ,Hydrogen peroxide ,chemistry.chemical_classification ,biology ,Mercury ,Peroxides ,Mercury (element) ,Glucose ,Enzyme ,Lead ,Peroxidases ,chemistry ,Spectrophotometry ,visual_art ,Gluconic acid ,biology.protein ,visual_art.visual_art_medium ,Nuclear chemistry - Abstract
The inhibition of the glucose oxidase enzyme by three heavy metal inhibitors, Ag(I), Hg(II), and Pb(II) is reported. The method for the microdetermination of these metals is based on the decreased rate of the enzyme-catalyzed, aerobic oxidation of glucose to gluconic acid and hydrogen peroxide. In the presence of horseradish peroxidase, the rapid oxidation ofo-dianisidine by hydrogen peroxide can be followed spectrophotometrically at 440 nm. Silver(I) has been determined in the range of 0.005 to 0.2μg/ml with an absolute error of less than 14 ng. Mercury(II) has been determined in the range of 0.1 to 0.4μg/ml with a maximum error of 34 ng. Below a concentration of 260μg/ml Pb(II), no appreciable inhibition was observed. Results indicate the utility of enzymatic methods to the trace determination of metal ion inhibitors.
- Published
- 1968