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The structure and immunochemical properties of lipopolysaccharide from Budvicia aquatica 97U124, a representative of a novel species of Enterobacteriaceae, were studied. The O-polysaccharide (OPS) was isolated by mild acid hydrolysis and characterized by monosaccharide analysis and NMR spectroscopy. It was shown that the OPS from B. aquatica 97U124 consisted of repeating units with the structure [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]

The lipopolysaccharide of a new species of Enterobacteriaceae, Rahnella aquatilis 95U003, was isolated and investigated. The structural components of the lipopolysaccharide molecule, lipid A, core oligosaccharide, and O-specific polysaccharide, were isolated by mild acidic hydrolysis. In lipid A, 3-hydroxytetradecanoic (64.3%) and tetradecanoic (22.3%) acids were found to be predominant fatty acids. In fractions 1 and 2 of the core oligosaccharides, galactose (36.6 and 43.6%), mannose (35.5 and 23.5%), and glucose (42.1 and 25.3%) were shown to be the major monosaccharides. The O-specific polysaccharide consisted of regularly repeating hexasaccharide units of the following structure: [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]

The lipopolysaccharide (LPS) from a new Enterobacteriaceae species, Rahnella aquatilis 2-95, was isolated and investigated. The structural components of the LPS molecule, namely, lipid A, core oligosaccharide, and O-specific polysaccharide, were obtained by mild acid hydrolysis. In lipid A, 3-oxytetradecanoic and tetradecanoic acids were found to be the predominant fatty acids. The major monosaccharides of the core oligosaccharide were galactose, arabinose, fucose, rhamnose, and an unidentified component. The O-specific polysaccharide was found to be assembled of a repeated trisaccharide unit of the following structure: [Figure not available: see fulltext.] The R. aquatilis 2-95 LPS is less toxic and more pyrogenic than the LPS from the R. aquatilis 1-95 strain studied earlier. Both acyl and phosphate groups are essential for toxic and pyrogenic activity of R. aquatilis 2-95 LPS. [ABSTRACT FROM AUTHOR]

The composition, structure, and certain biological properties of lipopolysaccharides (LPS) isolated from six strains of bacteria Pseudomonas syringae pv. atrofaciens pathogenic for grain-crops (wheat, rye) are presented. The LPS-protein complexes were isolated by a sparing procedure (extraction from microbial cells with a weak salt solution). They reacted with the homologous O sera and contained one to three antigenic determinants. Against the cells of warm-blooded animals (mice, humans) they exhibited the biological activity typical of endotoxins (stimulation of cytokine production, mitogenetic activity, etc.). The LCD of the biovar type strain was highly toxic to mice sensitized with D-galactosamine. The structural components of LPS macromolecules obtained by mild acidic degradation were characterized: lipid A, core oligosaccharide, and O-specific polysaccharide (OPS). Fatty acids 3-HO-C10:0, C12:0, 2-HO-C12:0, 3-HO-C12:0, C16:0, C16:1, C18:0, and C18:1 were identified in lipid A of all the strains, as well as the components of the hydrophilic part: glucosamine (GlcN), ethanolamine (EtN), phosphate, and phosphoethanolamine (EtN-P). In the core LPS, glucose (Glc), rhamnose (Rha), L-glycero-D-manno-heptose (Hep), GlcN, galactosamine (GalN), 2-keto-3-deoxy-D-mannooctonoic acid (KDO), alanine (Ala), and phosphate were present. The O chain of all the strains consisted of repeated elements containing a linear chain of three to four L-(two strains) or D-Rha (four strains) residues supplemented with a single residue of 3-acetamido-3,6-dideoxy-D-galactose (D-Fuc p3Nac), N-acetyl-D-glucosamine (D-Glc pNAc), D-fucose (D-Fuc f), or D-Rha p (strain-dependent) as a side substituent. In different strains the substitution position for Rha residues in the repeated components of the major rhamnan chain was also different. One strain exhibited a unique type of O-chain heterogeneity. Immunochemical investigation of the LPS antigenic properties revealed the absence of close serological relations between the strains of one pathovar; this finding correlates with the differences in their OPS structure. Resemblance between the investigated strains and other P. syringae strains with similar LPS structures was revealed. The results of LPS analysis indicate the absence of correlation between the OPS structure and the pathovar affiliation of the strains. [ABSTRACT FROM AUTHOR]

The lipopolysaccharide from the freshwater bacterium Rahnella aquatilis 1-95 has been isolated and investigated for the first time. The structural components of the lipopolysaccharide molecule, such as lipid A, core oligosaccharide, and O-specific polysaccharide, were isolated by mild acidic hydrolysis. In lipid A, 3-hydroxytetradecanoic and tetradecanoic acids were found to be the predominant fatty acids. In the core, oligosaccharide, galactose, arabinose, fucose, and an unidentified component were shown to be the major monosaccharides. The O-specific polysaccharide consists of a regularly repeating trisaccharide unit with the following structure: . Both acyl and phosphate groups have been shown to be responsible for the toxic and pyrogenic properties of the lipopolysaccharide of R. aquatilis. [ABSTRACT FROM AUTHOR]