1. Production of viral progeny in insect cells undergoing apoptosis induced by a mutant Anticarsia gemmatalis nucleopolyhedrovirus.
- Author
-
de Castro ME and Ribeiro BM
- Subjects
- Animals, Cell Line, DNA Replication, DNA, Viral analysis, DNA, Viral genetics, Genome, Viral, Insecta cytology, Microscopy, Phase-Contrast, Mutation, Nucleopolyhedroviruses genetics, Nucleopolyhedroviruses metabolism, Pest Control, Biological, Viral Proteins biosynthesis, Apoptosis physiology, Insecta virology, Nucleopolyhedroviruses growth & development, Virus Replication physiology
- Abstract
The Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) is the most successful viral biopesticide in use worldwide. We have demonstrated that despite widespread apoptosis and no protein synthesis at 48 h p.i., UFL-AG-286 cells infected with a mutant of AgMNPV (vApAg), produced significant amounts of budded virus (BVs) and viral DNA late in infection. However, a different susceptible cell line (BTI-Tn5B 1-4) showed no signs of apoptosis and produced 3.5 times more budded virus when infected with vApAg. A comparison of DNA from AgMNPV and vApAg digested with the same restriction enzymes showed differences in the restriction pattern, indicating that the vApAg phenotype might be due to a mutation in a gene or genes responsible for directly or indirectly inhibiting apoptosis in UFL-AG-286 cells.
- Published
- 2001
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