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Your search keyword '"Yang, Ling"' showing total 21 results
Start Over Author "Yang, Ling" Journal microbial pathogenesis
21 results on '"Yang, Ling"'

19. Serotypes and virulence genes of Pseudomonas aeruginosa isolated from mink and its pathogenicity in mink

Author

Wang Nan, Guo Wan-li, Zhu Jie, Zhang Bo-shun, Jiang Shi-jin, Li Han, Peng Hui, Yang Ling-zhi, Zhu Qian, and Xie Zhi-jing

Subjects
0301 basic medicine, Serotype, Virulence Factors, 030106 microbiology, Virulence, medicine.disease_cause, Microbiology, 03 medical and health sciences, Bacterial Proteins, biology.animal, Drug Resistance, Multiple, Bacterial, medicine, Animals, Pseudomonas Infections, Mink, Cross-resistance, Phylogeny, biology, Pseudomonas aeruginosa, Inoculation, Antimicrobial, Anti-Bacterial Agents, Bacterial Typing Techniques, Multiple drug resistance, 030104 developmental biology, Infectious Diseases
Abstract

In this study, 20 P. aeruginosa strains were isolated from 112 farmed mink exhibiting hemorrhagic pneumonia in mideastern Shandong province, China. Serotype G (18/20) was the dominant serotype among the isolates with prevalence in mink, followed by serotype B (1/20), serotype C (1/20). The 9 virulence-associated genes of P. aeruginosa were tested using PCR. The prevalence of the virulence genes for the isolates were algD 95% (19/20), plcH 85% (17/20), exoY 80% (16/20), aprA 75% (15/20), lasB 70% (14/20), exoS 65% (13/20), exoT 60% (12/20) and toxA 60% (12/20), respectively. The 20 isolates were negative for exoU gene. The isolates exhibited multidrug resistance and cross resistance, using antimicrobial disc susceptibility assays. The animal experiments demonstrated that LD50% of the P.aeruginosa–CS–2 in the intratracheally challenged mink was 2.2 × 107.0 CFU, and 6.8 × 104.0 CFU in the intraperitoneally challenged mink. It implied that both the inoculation doses and the routes of inoculation could have influences on the pathogenicity of P. aeruginosa in mink. Therefore, the evolutionary and epidemiological surveillance of P. aeruginosa in mink should be further strengthened for public health.

Published
2019

21. Metagenomic analysis reveals antibiotic resistance genes in the bovine rumen.

Author

Jing R and Yan Y

Subjects
Angiotensin Receptor Antagonists, Angiotensin-Converting Enzyme Inhibitors, Animals, Anti-Bacterial Agents pharmacology, Cattle, Drug Resistance, Microbial, Genes, Bacterial genetics, Humans, Rumen, Metagenome, Metagenomics
Abstract

Metagenomics and network analysis were used to profile antibiotic resistance genes (ARGs) and their cooccurrence patterns in bovine rumen microbes. A total of 4941 ruminal microbial genomes and 20 metagenome samples were used in this study. In general, 103 ARG subtypes belonging to 20 ARG types in 79 candidate genomes were identified, showing the broad-spectrum profiles of ARGs in the bovine rumen environment. A wide distribution of genes encoding bacitracin resistance was found among the candidate genomes, suggesting the possibility that bovines might be one of the sources of bacitracin resistance genes. Cooccurrence patterns were found within or between the ARG types, and a positive correlation was found between some ARGs and bacteria, which revealed potential dominant hosts of ARGs. The investigation showed that bovine rumen systems are important ARG reservoirs, and our research might provide a theoretical basis for the evaluation of the harmfulness of ARGs and antibiotic-resistant bacteria (ARB) to food safety and human health., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Published
2020
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