1. Effects of Hemagglutination Activity in the Serum of a Deep-Sea Vent Endemic Crab, Shinkaia Crosnieri, on Non-Symbiotic and Symbiotic Bacteria
- Author
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Fujiyoshi, So, Tateno, Hiroaki, Watsuji, Tomoo, Yamaguchi, Hideyuki, Fukushima, Daisuke, Mino, Sayaka, Sugimura, Makoto, Sawabe, Tomoo, Takai, Ken, Sawayama, Shigeki, and Nakagawa, Satoshi
- Subjects
Serum ,Erythrocytes ,Bacteria ,Self/non-self recognition ,Hemagglutination ,Articles ,Deep-sea hydrothermal field ,Bacterial Physiological Phenomena ,Host-microbe interaction ,Hydrothermal Vents ,Decapoda ,Animals ,Hemagglutinin/lectin ,Horses ,Rabbits ,Symbiosis - Abstract
In deep-sea hydrothermal environments, most invertebrates associate with dense populations of symbiotic microorganisms in order to obtain nutrition. The molecular interactions between deep-sea animals and environmental microbes, including their symbionts, have not yet been elucidated in detail. Hemagglutinins/lectins, which are carbohydrate-binding proteins, have recently been reported to play important roles in a wide array of biological processes, including the recognition and control of non-self materials. We herein assessed hemagglutination activity in the serum of a deep-sea vent endemic crab, Shinkaia crosnieri, which harbors chemosynthetic epibionts on its plumose setae. Horse and rabbit erythrocytes were agglutinated using this serum (opt. pH 7.5 and opt. temperature 15°C). Agglutinating activity was inhibited by eight kinds of sugars and several divalent cations, did not require any divalent metal ions, and remained detectable even after heating the serum at 100°C for 30 min. By using fluorescently labeled serum, we demonstrated that deep-sea crab serum components bound to the epibionts even in the presence of sugars. This study represents the first immunological assessment of a deep-sea vent endemic crab and demonstrated the possibility of a non-lectin-mediated symbiont-host interaction.
- Published
- 2015