1. PCR with sequence-specific primers for typing of diallelic blood groups.
- Author
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Rink G, Scharberg EA, and Bugert P
- Subjects
- Alleles, Base Sequence, Electrophoresis, Agar Gel methods, Genotype, Humans, Blood Group Antigens genetics, DNA Primers genetics, Genotyping Techniques methods, Polymerase Chain Reaction methods, Polymorphism, Single Nucleotide
- Abstract
PCR with sequence-specific primers (PCR-SSP) is a cost-effective and robust method for the analysis of single nucleotide polymorphisms (SNPs). Many blood group antigens and the antithetic antigens are based on a diallelic SNP in the coding region of the corresponding blood group gene. Here, we describe PCR-SSP protocols for genotyping 24 blood group antigens based on 12 diallelic SNPs. We also provide protocols for molecular determination of the rare blood group phenotypes Yk(a-) and Vel-.
- Published
- 2015
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