1. Methods to Evaluate AMPK Regulation of Macrophage Cholesterol Homeostasis.
- Author
-
LeBlond ND and Fullerton MD
- Subjects
- Animals, Atherosclerosis pathology, Cell Line, Culture Media, Conditioned, Lipid Metabolism drug effects, Primary Cell Culture, AMP-Activated Protein Kinases metabolism, Cholesterol metabolism, Enzyme Activation drug effects, Enzyme Activators pharmacology, Macrophages metabolism
- Abstract
Macrophages are a driving force in the development and progression of atherosclerosis, a chronic condition that can lead to cardiovascular disease. In this chapter we describe methods that monitor macrophage cholesterol homeostasis such as cholesterol synthesis, uptake, and efflux, all with the use of AMPK activators and potential genetic models that could help shed light on the role of this metabolic regulator in atherosclerosis and other chronic diseases.
- Published
- 2018
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