1. Analyzing membrane dynamics with live cell fluorescence microscopy with a focus on yeast mitochondria.
- Author
-
Scholz D, Förtsch J, Böckler S, Klecker T, and Westermann B
- Subjects
- Gene Expression Regulation, Fungal, Luminescent Proteins genetics, Luminescent Proteins metabolism, Saccharomyces cerevisiae cytology, Saccharomyces cerevisiae genetics, Microscopy, Fluorescence methods, Mitochondrial Membranes metabolism, Saccharomyces cerevisiae metabolism
- Abstract
With the availability of increasing numbers of fluorescent protein variants and state-of-the-art imaging techniques, live cell microscopy has become a standard procedure in modern cell biology. Fluorescent markers are used to visualize the dynamic processes that take place in living cells, including the behavior of membrane-bound organelles. Here, we provide two examples of how we analyze the membrane dynamics of mitochondria in living yeast cells using wide field and confocal microscopy: (1) Long-term observation of mitochondrial shape changes using mitochondria-targeted fluorescent proteins and (2) monitoring the behavior of individual mitochondria using a mitochondria-targeted version of a photoconvertible fluorescent protein.
- Published
- 2013
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