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Start Over Author "Ruyue HE" Journal methods in molecular biology (clifton, n.j.)
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1. In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion

Author

Yinghui, Liu, Ruyue, He, Min, Zhu, and Haijia, Yu

Subjects
Biological Transport, SNARE Proteins, Membrane Fusion
Abstract

The GLUT4 vesicle fusion is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and a variety of regulatory proteins. For example, synip and tomosyn negatively regulate GLUT4 SNARE-mediated membrane fusion. Here we describe in vitro reconstituted assays to determine the molecular mechanisms of SNAREs, synip, and tomosyn. These methods can also be extended to the studies of other types of membrane fusion events.

Published
2022

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