1. Detecting apoptosis in Drosophila tissues and cells
- Author
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Richa Arya, Allison K. Timmons, Kristin White, Eltyeb Abdelwahid, and Tatevik Sarkissian
- Subjects
TUNEL assay ,biology ,Acridine orange ,Apoptotic DNA fragmentation ,Apoptosis ,Mitochondrion ,Molecular biology ,Article ,Permeability ,General Biochemistry, Genetics and Molecular Biology ,Mitochondria ,Cell biology ,chemistry.chemical_compound ,chemistry ,Caspases ,biology.protein ,Animals ,Caspase 10 ,Drosophila ,Mitochondrial fission ,Molecular Biology ,Caspase ,Developmental Biology - Abstract
In this chapter we discuss methods that can be used to study apoptotic cell death in the Drosophila embryo, ovary, as well as in cultured cell lines. These methods assay various aspects of the cell death process, from mitochondrial changes to caspase activation and DNA cleavage. The assays are useful for examining apoptosis in normal development and in response to developmental perturbations and external stresses. These techniques include Acridine Orange staining, TUNEL, cleaved caspase staining, caspase activity assays and assays for mitochondrial fission and permeabilization.
- Published
- 2014
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