1. Tissue localization of selenium of parental or dietary origin in rainbow trout (Oncorhynchus mykiss) fry using LA-ICP MS bioimaging
- Author
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Pierre-André Geraert, Philip Antony Jesu Prabhu, Pauline Wischhusen, Stéphanie Fontagné-Dicharry, Benoit Fauconneau, Sadasivam Kaushik, Mickael Briens, Germain Vallverdu, Sandra Mounicou, Maïté Bueno, Carine Arnaudguilhem, Brice Bouyssiere, Nutrition, Métabolisme, Aquaculture (NuMéA), Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Adisseo France SAS, F-92160 Antony, Partenaires INRAE, Institute of Marine Research [Bergen] (IMR), University of Bergen (UiB), Aquitaine Region [AQUITRACES project n°20131206001-13010973], I-site E2S: Energy and Environmental Solutions from the University of Pau and Pays de l’Adour, and ANR-11-RSNR-0002,AMORAD,AMORAD1(2011)
- Subjects
0301 basic medicine ,Male ,food.ingredient ,Offspring ,Biophysics ,chemistry.chemical_element ,Broodstock ,Aquaculture ,010501 environmental sciences ,Biology ,01 natural sciences ,Biochemistry ,Mass Spectrometry ,Biomaterials ,03 medical and health sciences ,Selenium ,Animal science ,food ,Dietary Sodium ,La icp ms ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,Yolk ,Animals ,14. Life underwater ,Selenomethionine ,0105 earth and related environmental sciences ,Metals and Alloys ,Total body ,Animal Feed ,030104 developmental biology ,chemistry ,Chemistry (miscellaneous) ,Oncorhynchus mykiss ,Dietary Supplements ,Rainbow trout ,Female ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition - Abstract
In relation to the decrease of selenium (Se) content in aquafeeds, the impact of level and form of parental and dietary Se supplementation was investigated in rainbow trout fry using laser ablation-inductively coupled plasma mass spectrometry (LA-ICP MS) bioimaging. The offspring of rainbow trout broodstock, fed either a control diet without any Se supplementation (0.3 mg Se/kg diet) or a diet supplemented with Se (0.6 mg Se/kg diet) either as sodium selenite or hydroxy-selenomethionine, were sampled at swim-up fry stage or after 11 weeks of cross-feeding. Total body Se levels were influenced by parental Se nutrition in swim-up fry and by direct Se feeding in 11-week fry with higher levels in the Se-supplemented groups compared with the control and the highest levels in the hydroxy-selenomethionine treatment. The Se retention was lower for dietary sodium selenite. Selenomethionine levels increased when Se was provided as hydroxy-selenomethionine. LA-ICP MS maps revealed yolk in swim-up fry and intestine, liver, and kidney in 11-week fed fry as tissues with high Se abundance. In swim-up fry, muscle Se was the highest abundant when parents were fed hydroxy-selenomethionine. In 11-week fed fry, muscle Se abundance was higher in the head part of fry fed both Se-supplemented diets, but only in the tail part of fry fed hydroxy-selenomethionine. Liver Se abundance was higher in fry fed sodium selenite compared with the control diet supporting the hypothesis that tissue Se distribution can be influenced by parental and dietary Se forms and levels.
- Published
- 2020
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