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[Display omitted] The synthesis of glycoprobes based on water-soluble polyacrylamide with a custom content of any low molecular weight fluorescent label is described. The synthesis is carried out in a stepwise manner: in the first step, the polymer is strictly dose-modified with a side substituent-linker containing an amino group; in the second step, the amino group is quantitatively acylated with an activated ester of the dye. The probes are designed to identify and study carbohydrate-binding proteins of plant cells. [ABSTRACT FROM AUTHOR]

[Display omitted] An approach to the design of pseudocyclic peptide epitopes is proposed: lipid tails are attached to both C- and N-termini of the peptide through hydrophilic spacers; thanks to two lipid anchors, the conjugate is inserted into the membrane at two sites simultaneously, and the peptide becomes conformation- constrained. Despite the presence of two long lipid regions, the high hydrophilicity of the spacer makes the entire structure water-soluble, which is crucial for integration into living cells. [ABSTRACT FROM AUTHOR]

The synthetic approach to 6''SiaLec and its 6-O-Su derivative comprised α-sialylation of a protected Lec derivative, 2,3-di-OAcGalβ1-3(3-OAc-6-OBn)GlcNAcβ1-Osp (68% yield) as the key stage. Deprotection of the obtained trisaccharide (three stages, 79% overall yield) led to Neu5Acα2-6Galβ1-3GlcNAcβ1-Osp; partial deprotection followed by selective sulfation and total deprotection – to Neu5Acα2-6Galβ1-3(6-O-Su)GlcNAcβ1-Osp (four stages, 72% overall yield). [Copyright &y& Elsevier]