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A family of glucose transporters (GLUT) mediates the cellular uptake of glucose at the plasma membrane by facilitated diffusion. We investigated the presence of isoforms GLUT1-4 of class I subfamilies in different types of cells in the mouse, rat and human testis by indirect immunofluorescence technique. Immunocytochemical analyses demonstrated that GLUT1 was expressed in the rat testis, GLUT2 in the mouse and rat testis, GLUT3 in the mouse, rat and human testis and GLUT4 was not presented in the testis at all. A very intensive positive immunoreaction for GLUT3 was found in Sertoli cells, peritubular myoid cells, macrophage-like interstitial cells, testicular endothelial cells and early spermatocytes. GLUT3 positive cells were not found in the luminal part of Sertoli cells, spermatids or Leydig cells. The present results suggest that glucose uptake in different testicular cells is mediated by GLUT1, GLUT2 and GLUT3 and the GLUT3 was the prominent glucose transporter type in the testicular cells.

Objective: Leukocyte migration plays an important role in inflammation. The aim of our study was to detect the activated macrophage count and to define the apoptotic cell count in the rat's liver and lungs during different stages of sepsis and to clarify whether the activity of macrophages is associated with the apoptotic cell count in the course of the septic process., Material and Methods: Experimental sepsis was induced by inoculating Wistar rats intraperitoneally with E. coli cells. The liver and lung tissue was obtained 2, 6, 24, 48 and 120 hours after inoculation, and blood smears to detect the leukocyte volume were prepared at the same time. Macrophage activity was studied by immunohistochemical staining, using the ABC method. Apoptotic cell count was detected with the "In Situ Cell Death Detection Kit"., Results: Decrease in the total count of leukocyte and lymphocyte percentage and a rise in the percentage of neutrophils in the blood were evidences of a strong inflammation process in an organism. The count of activated macrophages in the liver was high, showing the maximum level at the end of the 2(nd) h after inoculation, after which it began to fall. Apoptotic cell count rose after a decrease in macrophage activity. In the lungs both changes - a decrease in activated macrophage level and a rise in the apoptotic cell count took place later on., Conclusion: Our investigations indicate that a decrease in the activated macrophage level is connected with an increased rise in the apoptotic cell count in both organs depending on the stage of the disease and occurs in the liver earlier than in the lungs but the process of cell apoptosis was more intensive in the lungs.