2 results on '"specific IgA"'
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2. Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice
- Author
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Maria Jimena Abrey-Recalde, Maria Pilar Mejias, Leticia V. Bentancor, Ariela Baschkier, Marta Rivas, María Victoria Ramos, Romina Jimena Fernandez-Brando, Cecilia Analia Panek, Elizabeth Miliwebsky, Gabriel Cabrera, and Marina S. Palermo
- Subjects
Microbiology (medical) ,Male ,Serum ,CIENCIAS MÉDICAS Y DE LA SALUD ,T-Lymphocytes ,Serum Anti-Stx2 Antibodies ,Immunology ,Ciencias de la Salud ,Administration, Oral ,medicine.disease_cause ,Microbiology ,Mucosal Immune Response ,Feces ,Peyer's Patches ,Immune system ,STX2 ,hemic and lymphatic diseases ,medicine ,Ehec ,Immunology and Allergy ,Mesenteric lymph nodes ,Animals ,Intestinal Mucosa ,Escherichia coli ,B cell ,B-Lymphocytes ,Mice, Inbred BALB C ,biology ,Shiga-Toxigenic Escherichia coli ,Escherichia coli Vaccines ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,Acquired immune system ,Antibodies, Bacterial ,Specific Iga ,Immunoglobulin A ,Enfermedades Infecciosas ,Disease Models, Animal ,medicine.anatomical_structure ,Lymphatic system ,Blood ,Immunoglobulin G ,Hemolytic-Uremic Syndrome ,biology.protein ,bacteria ,Female ,Antibody - Abstract
Hemolytic uremic syndrome (HUS) is the major complication of gastrointestinal infections with enterohemorrhagic Escherichia coli (EHEC) and is mediated by the production of Shiga toxins (Stx). Although it has been previously reported that not only HUS patients but healthy children have anti-Stx antibodies, very little is known about how these infections impact on mucosal immune system to generate a specific immune response. This work aimed to evaluate the immune responses elicited after a single oral dose of EHEC in a mouse model of HUS at weaning. We found sequential activation of T and B lymphocytes together with an increased percentage of IgA-bearing B cells in Peyer´s patches and mesenteric lymph nodes. We also found fecal anti-EHEC IgA and serum anti-Stx2 IgG in EHEC-inoculated mice. Besides, these mice were partially protected against an intravenous challenge with Stx2. These data demonstrate that one episode of EHEC infection is enough to induce activation in the gut-associated lymphoid tissue, especially the B cell compartment, and lead to the production of specific IgA in mucosal tissue and the generation of systemic protection against Stx2 in a percentage of intragastrically inoculated mice. These data also support the epidemiologic observation that a second episode of HUS is very rare. Fil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Cabrera, Gabriel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Baschkier, Ariela. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Mejias, María Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Panek, Cecilia Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Miliwebsky, Elizabeth. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Abrey Recalde, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Bentancor, Leticia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Ramos, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Rivas, Marta. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
- Published
- 2013
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