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The study of intestinal microbiota in vector insects like triatomines is paramount in parasitology because many parasitic species inhabit the vector's gut. Although knowledge on the gut microbiota in various vectors of the parasitic flagellate Trypanosoma cruzi has grown, research efforts have focused on South American triatomines. This study reports the isolation of bacterial microbiota in the anterior and posterior gut of Meccus pallidipennis (a triatomine species endemic to Mexico) by culture, as well as its identification by phenotypic and biochemical tests and its quantification by counting colony‐forming units. The study was performed on fifth‐instar nymph and adult specimens of M. pallidipennis, either laboratory‐bred or collected in the field and either infected or not with T. cruzi. Overall, 17 bacterial species were identified, with the genera Bacillus and Staphylococcus being the most prevalent regardless of the origin of the insects. No differences were observed in the number of bacterial species in the gut of laboratory‐bred and field‐collected insects, neither with respect to life stage or infection status. In general, the Shannon‐Weaver diversity index was higher in non‐infected insects than in infected ones. Further studies using non‐culture methods are required to determine whether bacterial species diversity is modified by laboratory breeding. [ABSTRACT FROM AUTHOR]