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Culicoides biting midges (Diptera: Ceratopogonidae) are known vectors of multiple arboviruses of veterinary and medical importance, as well as avian haemosporidian parasites. Despite their significant role as vectors, the distribution of Culicoides species in non‐farmland habitats remains largely unknown. We conducted a study to assess the species community composition, abundance, and host feeding patterns of Culicoides biting midges in urban ecosystems in northern Spain. Light‐baited suction traps were operated biweekly for 6 months across six urban landscapes (three cemeteries and three green areas). In total, 181 Culicoides specimens of 18 species were morphologically identified, with the Obsoletus complex being predominant (43.% of the total catches), followed by Culicoides kibunensis (21.5%). Culicoides midges peaked in early June in green areas and in May in cemeteries. Host feeding patterns revealed avian preferences (mainly Turdus spp.) in four Culicoides species. Despite the low overall number of specimens collected, a high diversity of biting midge species was recorded. These findings offer critical insights into Culicoides ecology and their interactions with hosts in urban settings, which are vital for disease surveillance and management strategies. [ABSTRACT FROM AUTHOR]

Culicoides biting midges (Diptera: Ceratopogonidae) are vectors of pathogens that affect wildlife and livestock. Understanding the composition and distribution of vector species is crucial for implementing control strategies and preventing the spread of infectious diseases. This study provides a morphological and molecular characterisation of Culicoides caucoliberensis, which represents the first record for Spain, increasing the number of Culicoides species in the country to 85. A total of 213 specimens were collected using Onderstepoort‐ultraviolet down‐draught light traps on a rocky coastline in the Balearic Islands during two sampling periods in 2022. Phylogenetic analysis showed that C. caucoliberensis forms a monophyletic cluster within the Maritimus group. Host preferences were determined for the first time and showed propensity to feed on the European shag (Phalacrocorax aristotelis). The vector role of C. caucoliberensis for haemosporidian transmission remains unclear since molecular detection of Haemosporidians (Haemoproteus and Plasmodium) was negative for all the pools of parous and engorged females analysed. This study emphasises the importance of conducting entomofauna studies in lesser‐known Mediterranean islet landscapes and highlights the need for research on vectors within the One Health framework. [ABSTRACT FROM AUTHOR]

Mosquitoes (Diptera: Culicidae) are common bloodsucking Diptera frequently found in aquatic environments, which are valuable ecosystems for many animal species, particularly migrating birds. Therefore, interactions between these animal species and mosquitoes may play a critical role in pathogen transmission. During 2018–2019, mosquitoes were collected from two aquatic ecosystems in northern Spain using different methodologies and identified using classical morphology and molecular tools. A total of 1529 males and females of 22 native mosquito species (including eight new records for the region) were trapped using CO2‐baited Centers for Disease Control and Prevention (CDC) traps and sweep netting. Among the blood‐fed female mosquitoes, 11 vertebrate host species—six mammals and five birds—were identified using DNA barcoding. The developmental sites of eight mosquito species were determined across nine microhabitats, and 11 mosquito species were caught landing on humans. The flight period varied among mosquito species, with some peaking in the spring and others in the summer. Our study highlights the advantages of mosquito sampling using various techniques to comprehensively characterise species composition and abundance. Information on the trophic preferences, biting behaviour and influence of climatic variables on the ecology of mosquitoes is also provided. [ABSTRACT FROM AUTHOR]