1. Neurons derived in vitro from ES cells express homeoproteins characteristic of motoneurons and interneurons
- Author
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Serge Alonso, Patrick Carroll, Pierre Filippi, Yannick Renoncourt, and Vilma Arce
- Subjects
Central Nervous System ,Embryology ,PAX6 Transcription Factor ,Tyrosine 3-Monooxygenase ,Cellular differentiation ,LIM-Homeodomain Proteins ,Peripherins ,Retinoic acid ,Muscle Proteins ,Nerve Tissue Proteins ,Tretinoin ,Biology ,Choline O-Acetyltransferase ,Nestin ,Mice ,chemistry.chemical_compound ,Intermediate Filament Proteins ,Interneurons ,medicine ,Animals ,Paired Box Transcription Factors ,Eye Proteins ,Cells, Cultured ,Homeodomain Proteins ,Motor Neurons ,Membrane Glycoproteins ,Glutamate Decarboxylase ,Reverse Transcriptase Polymerase Chain Reaction ,Stem Cells ,Gene Expression Regulation, Developmental ,PAX7 Transcription Factor ,Neural crest ,Cell Differentiation ,Peripherin ,Embryonic stem cell ,Molecular biology ,DNA-Binding Proteins ,Repressor Proteins ,medicine.anatomical_structure ,nervous system ,chemistry ,embryonic structures ,Neuron ,Stem cell ,Biomarkers ,Transcription Factors ,Developmental Biology - Abstract
We have characterized different neuronal subpopulations derived from in vitro differentiation of embryonic stem (ES) cells using as markers the expression of several homeodomain transcription factors. Following treatment of embryo-like aggregates with retinoic acid (RA), Pax-6, a protein expressed by ventral central nervous system (CNS) progenitors is induced. In contrast, Pax-7 expressed in vivo by dorsal CNS progenitors, and erbB3, a gene expressed by neural crest cells and its derivatives, are almost undetectable. CNS neuronal subpopulations generated expressed combinations of markers characteristic of somatic motoneurons (Islet-1/2, Lim-3, and HB-9), cranial motoneurons (Islet-1/2 and Phox2b) and interneurons (Lim-1/2 or EN1). Molecular characterization of neuron subtypes generated from ES cells should considerably facilitate the identification of new genes expressed by restricted neuronal cell lineages.
- Published
- 1998
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